Inhibitory Activities ofCudrania tricuspidataLeaves on Pancreatic LipaseIn Vitroand LipolysisIn Vivo

Kim, Young Sook; Lee, Youngseop; Kim, Jung-Hyun; Sohn, Eunjin; Kim, Chan Sik; Lee, Yun Mi; Jo, Kyuhyung; Shin, Sodam; Song, Yoojin; Kim, Joo Hwan; Kim, Jin Sook

doi:10.1155/2012/878365

Cited by 31 publications

(28 citation statements)

References 24 publications

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“…Orlistat, which reduces dietary fat absorption by inhibiting pancreatic lipase, is currently on the global market [ 4 ]. Pancreatic lipase is a major enzyme involved in triglyceride absorption in the intestine, and inhibiting fat absorption from the diet is a target for treating obesity [ 5 ]. However, these drugs have many limitations due to adverse effects, such as stomach pain, steatorrhea, and headaches [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

Dohaekseunggi-tang extract inhibits obesity, hyperlipidemia, and hypertension in high-fat diet-induced obese mice

Sung

Kim

Choi

et al. 2014

BMC Complement Altern Med

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BackgroundDohaekseunggi-tang (DHSGT) is a traditional plant-based medicine prescribed to promote blood circulation and to treat obesity and hypertension. The present study aimed to identify potential anti-obesity activities of DHSGT extract.MethodsAnti-obesity, anti-hyperlipidemic, and anti-hypertensive effects of orally-administered DHSGT extract were evaluated in high-fat diet- (HFD)-induced obese mice. Serum biochemistry profiles and expression of diverse metabolic regulatory gene mRNAs in mouse visceral fat were assessed by RT-PCR. The effects of DHSGT on angiotensin-1 converting enzyme (ACE) and pancreatic lipase activities were determined using in vitro inhibition assays.ResultsOral DHSGT treatment reduced obese HFD C57BL/6 J mouse body weight, liver and adipose tissue mass, adipocyte size, and blood pressure versus untreated HFD mice. DHSGT also decreased serum total cholesterol, LDL-cholesterol, triglyceride, glucose, and leptin concentrations, and increased HDL-cholesterol and adiponectin levels in HFD mice. Furthermore, DHSGT markedly increased mRNA expression of peroxisome proliferator activated receptor-gamma, uncoupling protein-2, and adiponectin in visceral adipose tissue of HFD mice. In vitro tests revealed that DHSGT effectively inhibited porcine pancreatic lipase and ACE activities, with IC50 values of 7.58 mg/ml and 0.56 mg/ml, respectively.ConclusionsThese results validate traditional knowledge and suggest that DHSGT may be potentially useful for managing hyperlipidemia, hyperglycemia, hypertension, and obesity.

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Section: Introductionmentioning

confidence: 99%

Dohaekseunggi-tang extract inhibits obesity, hyperlipidemia, and hypertension in high-fat diet-induced obese mice

Sung

Kim

Choi

et al. 2014

BMC Complement Altern Med

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“…One such plant is Cudrania tricuspidata (CT), which is used in Korean folk medicine ( 25 – 27 ). One study reported that CT leaves inhibited lipase activity, reduced plasma triacylglycerol levels, and delayed lipid absorption in vivo ( 28 ). In addition, recent studies found that CT has anti-inflammation and antihyperglycemia effects ( 29 – 31 ).…”

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confidence: 99%

Cudrania tricuspidatawater extract improved obesity-induced hepatic insulin resistance indb/dbmice by suppressing ER stress and inflammation

Kim

Nam

Jun

et al. 2015

Food & Nutrition Research

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BackgroundObesity can play a role in the development of hepatic insulin resistance. Although the molecular mechanism of the association between obesity and hepatic insulin resistance is unclear, it has been reported that obesity leads to hepatic endoplasmic reticulum (ER) stress and inflammation, which can induce the development of insulin resistance in several tissues.ObjectiveIn this study, we investigated the associations between hepatic insulin resistance, ER, and inflammation in obesity and the effect of water extract from Cudrania tricuspidata leaves (CTL) on hepatic insulin resistance induced by ER stress and inflammation in db/db mice.DesignThe mice were randomly divided into four groups: a normal control group (C57BL/6J), a control group (C57BL/6J-db/db), a CTL 100 group (C57BL/6J-db/db mice fed a dietary supplement of 100 mg/kg of CTL), and a CTL 300 group (C57BL/6J-db/db mice fed a dietary supplement of 300 mg/kg of CTL). After 8 weeks, we performed an oral glucose tolerance test and the mice were sacrificed.ResultsThe C57BL/6J-db/db mice developed obesity and hyperglycemia, and the ER stress response and inflammation were activated in their livers. Interestingly, there was a marked decrease in the activation of the ER stress response and insulin resistance in the livers of the C57BL/6J-db/db mice treated with CTL due to decreases in the phosphorylation of eIF2α, IRE1α, and IRS-1 serine and decreases in the mRNA expression of ATF4, c-Jun N-terminal kinase, C/EBPα, and C/EBP homologous protein. Dietary supplementation with CTL also induced a statistically significant decrease in the expression of pro-inflammatory cytokines, C-reactive protein (CRP), and NF-κB phosphorylation.ConclusionsOverall, these results suggest that CTL can improve hepatic insulin resistance and hyperglycemia by controlling obesity-induced ER stress and inflammation in the liver and that CTL may be a useful agent in treating hyperglycemia.

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“…Previous human clinical trials have shown that lipase inhibitors from plants can effectively reduce fat absorption to suppress weight gain in obese patients [12][13][14][15] , the findings in this work are not only very helpful to well understand the molecular interactions between pancreatic lipase and polyphenols extracts from peanut shells, but also greatly contribute to the development of the natural effective inhibitors for the prevention of human obesity in future. Therefore, polyphenols extracts from peanut shells may be useful in treatment of obesity.…”

Section: Resultsmentioning

confidence: 75%

Inhibitory Effect of Polyphenols Extracts from Peanut Shells on the Activity of Pancreatic Lipase in vitro

Yu¹,

Sun²,

Gao³

2014

Asian J. Chem.

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Obesity is a major health problem worldwide and is associated with several pathological disorders, such as diabetes, cardiovascular disease and cancer. Lipase inhibition is one of the main therapies for obesity. Recent studies have shown the role of polyphenols in the prevention of obesity and obesity-related chronic diseases. Here, we evaluated the ability of polyphenol-rich extracts from peanut shell to inhibit pancreatic lipase activity in vitro. The results showed that the inhibition ratio of polyphenols extracts from peanut shells (PPS) on lipase can reached 41.5 % at 0.29 mg/mL. And the inhibition type of polyphenols extracts from peanut shells against pancreatic lipase is competitive inhibition. The inhibition constant Ki was 0.16 mg/mL. Therefore, polyphenols extracts from peanut shells may be useful in treatment of obesity.

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Inhibitory Activities ofCudrania tricuspidataLeaves on Pancreatic LipaseIn Vitroand LipolysisIn Vivo

Cited by 31 publications

References 24 publications

Dohaekseunggi-tang extract inhibits obesity, hyperlipidemia, and hypertension in high-fat diet-induced obese mice

Dohaekseunggi-tang extract inhibits obesity, hyperlipidemia, and hypertension in high-fat diet-induced obese mice

Cudrania tricuspidatawater extract improved obesity-induced hepatic insulin resistance indb/dbmice by suppressing ER stress and inflammation

Inhibitory Effect of Polyphenols Extracts from Peanut Shells on the Activity of Pancreatic Lipase in vitro

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