Inhibitory activities of three Malaysian edible seaweeds on lipase and α-amylase

Alpha-amylase, antioxidant, and anti-inflammatory activities of Eucheuma denticulatum (N.L. Burman) F.S. Collins and Hervey

“…To date, the huge potential usage of this species as an important resource for health benefits and functional food is still untapped. In our previous study (Balasubramaniam et al 2013), E. denticulatum has been shown to inhibit α-amylase enzyme activity. The current study furthered our previous research on the impact of E. denticulatum seaweed fractions on α-amylase activity by fractioning the crude extracts using solvents of varying polarities (n-hexane, ethyl acetate, and acetone).…”

“…Seaweeds such as Rhodophyta have previously been noted for their abundance of metabolites and diversity compared to other seaweeds (Munro and Blunt 2005), including a diverse type of chemical constituents, namely from polyphenols, polysaccharides, and pigments to vitamins and minerals (Mohamed et al 2005;Sokolova et al 2011;Balasubramaniam et al 2013) which may contribute to the important biological effects such as antidiabetic, antioxidant, and anti-inflammatory. In our previous finding (Balasubramaniam et al 2013), E. denticulatum (red seaweed) have shown to have a good inhibitory potential on carbohydrate metabolizing enzymes, α-amylase.…”

“…The fresh seaweed samples were cleaned and processed as previously described (Balasubramaniam et al 2013). All cleaned samples were freeze-dried and ground to a fine powder and stored in air-tight containers at 80°C prior to extraction.…”

“…The α-amylase inhibition was determined based on a colorimetric assay using 96-well microplate as previously described (Balasubramaniam et al 2013). Briefly, 30 μL of test samples (10 mg mL −1 ) in 30 mM Sorensen's phosphate buffer (pH 7) was added to 30 μL of α-amylase solution (0.5 mg mL −1 ) in the same buffer and was pre-incubated at 37°C for 10 min.…”

Characterising a novel regulator of dietary fat digestion as a potential obesity treatment; from microplate to synthetic gut system

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Newcastle upon Tyne, Tyne and Wear NE2 4HH, UK Sodium alginate derived from seaweed has demonstrated pancreatic lipase inhibition in vitro (1) , and other seaweed extracts containing bioactive compounds have also shown a reduction in digestive enzyme activity (2) . Four extracts, containing biopolymers of Fucus vesiculosus were analysed for their potential to inhibit pancreatic lipase using modified turbidimetric methodology (3) and an olive oil substrate.

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