Inhibitory Activity of Mangiferin on Helicobacter Pylori-Induced Inflammation in Human Gastric Carcinoma Ags Cells

Abstract

Background: Gastric cancer is a serious health issue caused by H. pylori and claims more lives in developing and undeveloped countries. Hence, the need for a natural drug with several pharmacological activities with no adverse effect are highly recommended. The target of this study was to verify the anti-H. pyloric efficacy of mangiferin (MF) on H. pyloriinfected AGS cells. Materials and methods: AGS cells were co-cultured with H. pylori and incubated with increased concentration of MF (10, 20, 50 and 100 µg/mL) or amoxicillin (AMX) and DMSO (control) group to assess its anti-H. pyloric effect by checking inhibitory zone, bacterial drug sensitivity test (MIC and MBC), adhesion and invasive property and various inflammatory markers. Results: Co-culturing of H. pylori-infected AGS cells with MF (100 µg) considerably increased (p<0.05) the inhibitory zone as well as substantially lowered (p<0.05) in the levels of MBC and MIC with decreased adhesion and invasive property in a dose-dependent manner and thus endorsing its anti H. pyloric activity and are almost equivalent to antibiotic AMX. Meanwhile, inflammatory markers such as NF-κB subunit p65, interleukins-1β, IL-8, and TNF-α were also markedly suppressed (p<0.01) on treatment with MF. In addition, the protein expression of inflammatory enzymes like COX-2 and iNOS were notably downregulated (p<0.05) in AGS cells incubated with MF. Conclusion: We, concluded that MF treatment with H. pylori-infected AGS cells significantly suppressed the adhesion and invasion process as well as deactivated NF-p65 thereby blocking inflammatory response and thus lower the incidence of gastric carcinoma.

A Review on Antioxidant, Anti-Inflammatory and Gastroprotective Abilities of Mango (Magnifera indica) Leaf Extract and Mangiferin

“…Zhang and Yue demonstrated that mangiferin has the ability to eradicate H. pylori and concurrently inhibit multiple inflammatory markers including NF-Кβ subunit p65, IL-1β, IL-8 and TNF-α . In addition, the protein expression of inflammatory enzymes as COX-2 and iNOS were remarkably down-regulated in cultured human gastric carcinoma AGS cells following incubation with mangiferin [45]. The researchers concluded that mangiferin significantly downregulated the adhesion and invasion process as well as deactivated NF-p65, thereby blocking the inflammatory response and thus reducing the incidence of gastric carcinoma [45].…”

“…In addition, the protein expression of inflammatory enzymes as COX-2 and iNOS were remarkably down-regulated in cultured human gastric carcinoma AGS cells following incubation with mangiferin [45]. The researchers concluded that mangiferin significantly downregulated the adhesion and invasion process as well as deactivated NF-p65, thereby blocking the inflammatory response and thus reducing the incidence of gastric carcinoma [45]. Interestingly, in several other studies, mangiferin has demonstrated inhibitory activity against Helicobacter pylori-induced inflammatory responses in the gastrointestinal tissues and gastrointestinal toxicity [42][43][44]46].…”

Mangiferin enhances endochondral ossification‐based bone repair in massive bone defect by inducing autophagy through activating AMP‐activated protein kinase signaling pathway

“…Furthermore, MAG is also effective for preventing the production of hydroxyl radicals in the Fenton-type reaction (20). Previous researchers had proved that MAG has many other physiologic and pharmacological effects, such as anti-inflammatory (21), antidiabetic (22), anti-cancer (23), inhibition of osteoclast differentiation (24), and so on. However, the effects of MAG on autophagy and hypertrophic chondrocyte differentiation have not been studied.…”

Xanthone C-glycosides isomers purified from Dryopteris ramosa (Hope) C. Chr. with bactericidal and cytotoxic prospects

“…3 ). Mangiferin showed MIC 600 µg/mL against Helicobacter pylori as reported by Zhang and Yue (2017) . Khumpook et al (2018) demonstrated the antibacterial potential of three varieties of mango leaves against S. epidermidis, S. aureus, Methicillin-resistant S. aureus, P. acnes and P. aeruginosa .…”