Inhibitory effect and mechanism of Tagetes erecta L. fungicide on Fusarium oxysporum f. sp. niveum

Abstract

Botanical fungicides comprise attractive alternatives to chemical fungicides because of their environmental compatibility. Flavonoids extracted from Tagetes erecta L. have an inhibitory effect on fusarium wilt in watermelons caused by Fusarium oxysporum f. sp. niveum (FON). In this study, we synthesized one of these flavonoids, 2,5-dicyclopentylidene cyclopentanone (Tagetes erecta L. fungicide (TEF)) and assessed its activity against FON. In vitro, TEF inhibited FON growth and killed FON cells directly. TEF also affected FON cell physiology and mycelial structure. In watermelon plants with fusarium wilt, TEF protected the leaf cell structure and improved the germination rate of infected seeds while increasing overall plant resistance. A TEF-resistant mutant (FONM) was created by chemical mutagenesis. FON and FONM were analysed using iTRAQ and RNA-Seq, which identified 422 differentially expressed proteins and 7817 differentially expressed mRNAs in the proteome and transcriptome, respectively. The FONM mutations caused changes in the cell membrane and cell wall, which may constitute the site of action of TEF. Together, these results demonstrate that TEF could effectively control the watermelon fusarium wilt caused by FON, possibly through the inhibition of sterol biosynthesis. The data presented here suggest that TEF represents a new potential botanical anti-fungal drug.

The susceptibility of sea-island cotton recombinant inbred lines to Fusarium oxysporum f. sp. vasinfectum infection is characterized by altered expression of long noncoding RNAs

“…Fusarium oxysporum f. sp. vasinfectum ( Fov ) is a soil-borne plant fungal pathogen that causes vascular wilt disease through root infection in a wide range of plants, including economically important crops such as cabbage, banana, cotton, flax, watermelon, chickpea, and tomato 23–29 . These pathogens can exist in the form of mycelia, chlamydospores, or microsclerotia in soil and crop debris and can persist in the soil for 5–10 years before causing recurrent infection 27 .…”

Effects of Different Forms of Tagetes erecta Biofumigation on the Growth of Apple Seedlings and Replanted Soil Microbial Environment

“…Tagetes erecta L. is an annual herb of the genus Asteraceae, with antifungal, bacteriostatic, insecticidal and other biological activities [11,12]. Moreover, we noted the fungicidal effect of T. erecta on F. oxysporum [13], the main causative fungi of ARD, but the effect of its biofumigation on plant replanting disease has not been studied.…”

Extraction and characterization of cyclic lipopeptides with antifungal and antioxidant activities from Bacillus amyloliquefaciens

“…Scientists have developed many methods to control Fusarium wilt, including chemical pesticides, soil disinfection, rational crop rotation, cultivating disease‐resistant varieties (Han et al, 2019; Luo et al, 2019; Sun et al, 2015). Among them, chemical pesticides are the most effective and economical means to control Fusarium wilt (Chen et al, 2014; Gudmestad et al, 2013; Luo et al, 2019), but applying chemical pesticides has adverse effects on human health and the environment (Du et al, 2017; Jaber et al, 2020; Wang, Xu, et al, 2020). Compared to chemical fungicides, biological fungicides are sensitive to environmental conditions, mainly ultraviolet radiation and high temperature (do Nascimento et al, 2022; Glare et al, 2012).…”