Inhibitory effect for proliferation of oral bacteria in dogs by tooth brushing and application of toothpaste

Watanabe, Kazuhiro; S, Kijima; Nonaka, Chie; Matsukawa, Yuki; Yamazoe, Kazuaki

doi:10.1292/jvms.15-0277

Cited by 11 publications

(8 citation statements)

References 9 publications

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“…, Watanabe et al . ). As a result, other methods of plaque control such as dental hygiene chews or specifically formulated dental foods have been developed, with published evidence that they can reduce plaque accumulation.…”

Section: Introductionmentioning

confidence: 97%

“…Minimising plaque accumulation is the key means of preventing gingivitis and PD, and various methods of control have been marketed for the pet-owning public (Roudebush et al 2005). While tooth brushing has been long recognised as the gold standard control measure, there are well-recognised challenges associated with this method: notably, owner and patient compliance (Roudebush et al 2005, Harvey et al 2015, Watanabe et al 2016. As a result, other methods of plaque control such as dental hygiene chews or specifically formulated dental foods have been developed, with published evidence that they can reduce plaque accumulation.…”

Section: Introductionmentioning

confidence: 99%

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Prospective randomised blinded clinical trial assessing effectiveness of three dental plaque control methods in dogs

Allan¹,

Adams²,

Johnston

2018

J of Small Animal Practice

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Objectives To assess the effectiveness of three commonly used methods of controlling dental plaque accumulation within a general practice setting. Materials and Methods A blinded study, block randomising dogs at recruitment to receive one of three treatments to start after a routine scale and polish procedure: once daily tooth brushing using a toothbrush and veterinary toothpaste, once daily administration of a single dental hygiene chew or a prescription dental diet. Blinded scoring of plaque accumulation, both coverage and thickness, was performed at the end of the 6‐week study. Results A total of 22 dogs completed the study, which simultaneously assessed the effectiveness of the three means of plaque control. Daily tooth brushing was shown to be more than three times as effective at controlling plaque accumulation compared to using a daily dental chew or dental diet. The dental plaque scores for the diet and dental chews were not significantly different from one another and there was much greater variability in the mouth scores for these two groups of dogs compared to the tooth brushing group. Clinical Significance The study design has been shown to be an effective means of assessing plaque accumulation over a 6‐week period using a clean mouth model. Secondly, this study adds to the evidence which shows that daily tooth brushing is the most effective single method of reducing plaque accumulation and optimising dogs’ oral health.

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Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Prospective randomised blinded clinical trial assessing effectiveness of three dental plaque control methods in dogs

Allan¹,

Adams²,

Johnston

2018

J of Small Animal Practice

View full text Add to dashboard Cite

show abstract

“…Dental brush and rubber or silicone fingerstall were equally efficient in removing plaque, concluding that choice of instrument should be guided by the ease of handling by the dog owners. Watanabe et al, (2016) found that brushing significantly reduced the number of oral bacteria either alone or with use and toothpaste, in relation to the non-brushed group. Dentifrice was effective in the bacteria inhibition, but not exceeding the brushing, being its application advantageous and easy for the dogs' oral care (Watanabe et al, 2016) A good brushing technique may be associated with the use of dentifrice and oral antiseptic agents aiming to expand the oral hygiene benefits.…”

Section: Periodontal Disease and Oral Carementioning

confidence: 86%

“…Watanabe et al, (2016) found that brushing significantly reduced the number of oral bacteria either alone or with use and toothpaste, in relation to the non-brushed group. Dentifrice was effective in the bacteria inhibition, but not exceeding the brushing, being its application advantageous and easy for the dogs' oral care (Watanabe et al, 2016) A good brushing technique may be associated with the use of dentifrice and oral antiseptic agents aiming to expand the oral hygiene benefits. However, the fluoridated toothpaste commonly used by people is not suitable for dogs, because it can cause toxicity, since the animals ingest product during brushing, instead of spitting it (Gorrel et al, 2007).…”

Section: Periodontal Disease and Oral Carementioning

confidence: 86%

Natural plant products: an alternative to oral dog care?

Barbosa

Caetano

Lopes

et al. 2020

RSD

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Periodontal disease is one of the most common and prevalent oral diseases in dogs. With the increasing closeness between man and the animals of company, the care with the health and well-being of those animals also increase. Being the bacterial plaque the main cause of periodontal disease, oral care performed by dog owners are essential for the prevention and control of this disease. There are a lot of alternatives, developed exclusively for animal use, which assist in oral hygiene. There are data on the use of active natural products in the prevention of halitosis, dental plaque and calculus, in the form of antiseptic substances, but the volume of information is still scarce. Natural substances, used since immemorial times for treatment of diseases, have the potential to integrate oral care products, such as main or adjuvant active agent in the formulation of the most varied compounds. This is the result of desirable actions in oral and dental therapies, as anti-inflammatory, healing and antimicrobial. This article reviews the information available on the subject and seeks to indicate what is possible to do overcome the gap between research and application of these products on a day-to-day basis.

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“…Brushing has previously been determined to be an effective means of reducing plaque biofilm in vivo. 59,60 Sixty seconds was spent using the microbrush to disrupt the biofilm on the hydroxyapatite coated surface and the resulting solution was assessed using serial dilutions to determine relative CFU/mL between trials. The dilutions were spiral plated on Brucella agar and duplicate plates were incubated at 5% CO 2 for counts of Actinomyces canis and Neisseria canis and anaerobically for counts of Porphyromonas gulae (Tables 2 and 3).…”

Section: Methodsmentioning

confidence: 99%

Effect of TrisEDTA and Chlorhexidine 0.12% on an In Vitro-Defined Biofilm Representing the Subgingival Plaque Biofilm of the Dog

et al. 2021

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This study was designed to investigate the effects of chlorhexidine 0.12%, TrisEDTA (tromethamine ethylenediamintetraacetic acid), and a combination of chlorhexidine 0.12% and TrisEDTA on an in vitro plaque biofilm model comprised of three bacterial species commonly found in canine subgingival plaque. Porphyromonas gulae, Actinomyces canis, and Neisseria canis were grown in a biofilm on polished hydroxyapatite coated titanium alloy pucks for 72 h prior to exposure to one of four test solutions: TrisEDTA, chlorhexidine 0.12%, a combination of TrisEDTA and chlorhexidine 0.12%, or sterile deionized water as a control. Following exposure to the test solution, a sample was collected of the biofilm either immediately or following 24 h of additional incubation in a broth medium. Lower numbers of CFU/mL of Porphyromonas gulae resulted when the biofilm was treated with a solution of chlorhexidine 0.12% and TrisEDTA compared to with chlorhexidine 0.12% alone, TrisEDTA alone, or the control and so this solution can be said to be synergistic against Porphyromonas gulae in this controlled in vitro model. Greater reductions in the numbers of CFU/mL of Actinomyces canis and Neisseria canis resulted from treatment with chlorhexidine 0.12% alone than if treated with the combination of TrisEDTA and chlorhexidine 0.12%. When treated biofilm samples were allowed 24 h of additional growth in fresh media, greater variance resulted and this variance highlights the complex dynamics involved in bacterial growth within a biofilm.

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Inhibitory effect for proliferation of oral bacteria in dogs by tooth brushing and application of toothpaste

Cited by 11 publications

References 9 publications

Prospective randomised blinded clinical trial assessing effectiveness of three dental plaque control methods in dogs

Prospective randomised blinded clinical trial assessing effectiveness of three dental plaque control methods in dogs

Natural plant products: an alternative to oral dog care?

Effect of TrisEDTA and Chlorhexidine 0.12% on an In Vitro-Defined Biofilm Representing the Subgingival Plaque Biofilm of the Dog

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