Inhibitory Effect of 5-Alpha-Dihydroprogesterone on Nuclear Estrogen Receptor Binding of the Anterior Pituitary and Uterus in the Rat

Fuentes, Miguel; Muldoon, Thomas G.; Mahesh, Virendra B.

doi:10.1159/000125588

Cited by 7 publications

(5 citation statements)

References 28 publications

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“…In that study there appeared to be no changes in -actin mRNA levels at different times after estradiol administration, and the results were similar whether they were corrected using -actin or uncorrected for variations in transfer. Other tissue-specific differences in ER dynamics between the pituitary and the uterus have been reported by us earlier using progesterone [11,46], 5oL-pregnane-3,20-dione [22], and a5o-dihydrotestosterone and dexamethasone [16]. Furthermore, in the present study, ER mRNA levels in the uterus were increased after ovariectomy and decreased after estrogen priming, whereas in the anterior pituitary ER mRNA levels are decreased after ovariectomy and increased after estrogen priming [45].…”

Section: Discussionsupporting

confidence: 85%

“…Previous studies from our laboratory have demonstrated a dose-dependent decrease in occupied nuclear uterine ER in estrogen-primed, ovariectomized rats treated with either progesterone or 5a-dihydroprogesterone [11,22]. Dramatic decreases in uterine ER binding have also been observed after a single injection of estradiol by several investigators who have shown replenishment of the binding activity in 16-24 h [3, 6, 7, 23-25].…”

Section: Discussionmentioning

confidence: 91%

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Changes in Rat Uterine Estrogen Receptor Messenger Ribonucleic Acid Levels during Estrogen- and Progesterone-Induced Estrogen Receptor Depletion and Subsequent Replenishment1

Rosser

Chorich

Howard

et al. 1993

Biology of Reproduction

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The overall objectives of this study were to determine whether the rapid decrease in estrogen receptor (ER) binding in the rat uterus after an injection of estradiol and subsequent recovery of ER levels was accompanied by similar changes in ER mRNA levels. Furthermore, the effect of progesterone administered under conditions known to decrease ER binding, in the rat uterus, on ER mRNA levels was also investigated. Ovariectomy for 14 days brought about a 3-fold increase in rat uterine ER mRNA levels, and these elevated levels were decreased by a 3-day treatment with 2 micrograms of estradiol in ethanol/saline injected i.p. In the ovariectomized rat, 1 and 5 micrograms of estradiol brought about small but significant decreases in ER mRNA levels in 1 h, which did not parallel the rapid decrease in ER binding at that time reported earlier. The 10-micrograms dose of estradiol brought about a bigger decrease in ER mRNA levels. In the ovariectomized rat primed with estradiol (2 micrograms/day in ethanol/saline), the administration of 2 micrograms of estradiol brought about no change in uterine ER mRNA levels at 6 h as compared to the 1-h time point if the values were not corrected for beta-actin, which was significantly increased at 6 h. A dramatic increase in ER mRNA levels 12 h after the estradiol injection preceded the increase in ER binding observed at 18 h. Progesterone (0.8 mg/kg body weight [BW]) in the absence of estrogen priming brought about minimal but significant inhibition of ER mRNA levels 12 and 18 h after administration, with no effects at 1 and 6 h. In the presence of estrogen priming, the 0.8-mg/kg BW dose of progesterone did not cause any changes in ER mRNA levels beyond those brought about by estrogen alone after 1 h, even though it has been shown to significantly decrease ER binding. This was also true when a larger dose of progesterone (2.0 mg/kg BW) was used, a dose that decreases ER binding to a significantly greater extent than the 0.8-mg/kg BW dose. However, the 4.0-mg/kg BW dose of progesterone decreased ER mRNA levels. Thus a single injection of estradiol appears to cause a decrease in ER binding primarily by accelerated receptor processing and degradation with small changes in ER mRNA within the first hour. A significant increase in uterine ER mRNA at 12 h precedes increased ER binding at 18 h. This indicates new synthesis of ER during the recovery period.(ABSTRACT TRUNCATED AT 400 WORDS)

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Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 91%

Changes in Rat Uterine Estrogen Receptor Messenger Ribonucleic Acid Levels during Estrogen- and Progesterone-Induced Estrogen Receptor Depletion and Subsequent Replenishment1

Rosser

Chorich

Howard

et al. 1993

Biology of Reproduction

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“…In view of the uniqueness of these multiphasic actions of progesterone and differences in progesterone effects on E,R binding in the pituitary and the uterus, the studies were repeated using 5a-dihydroprogesterone, a metabolite of progesterone that utilizes the progesterone receptor (3 1). The pattern of effects of progesterone and Sa-dihydroprogesterone on pituitary and uterine E,R binding were similar (31).…”

Section: Discussionmentioning

confidence: 71%

Inhibitory Effect of Progesterone on Occupied Estrogen Receptors of Anterior Pituitary and Uterus in Adult Rats

Fuentes

Muldoon

Mahesh

1990

J Neuroendocrinology

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The acute effects of a single progesterone injection on estradiol receptor (E(2)R) binding in the anterior pituitary and uterus were examined in the mature ovariectomized rat. Adult ovariectomized female rats receiving estradiol were injected with various doses of progesterone followed 1 h later by a final injection of 2 mug of estradiol. All animals were sacrificed 1 h after estradiol and the E(2)R binding in the nuclei and cytosol was determined. In the anterior pituitary, progesterone decreased total nuclear E(2)R in a biphasic inhibitory pattern, with maximal effects at 0.8 and 4 mg/kg body wt doses of progesterone and a smaller decrease with the 2 mg/kg body wt dose. This progesterone-induced decrease of nuclear E(2)R was due to a decrease in the occupied form of nuclear E(2)R. The unoccupied nuclear E(2)R and cytosol E(2)R binding were not altered by progesterone administration. In the uterus, progesterone caused a dose-dependent decrease in total nuclear E(2)R binding. This effect also occurred in the occupied form of E(2)R. The uterine unoccupied nuclear E(2)R and cytosol E(2)R were not altered by progesterone administration. These studies emphasize differences in the tissue specificity of progesterone action on occupied nuclear E(2)R which are presumably responsible for the mediation of estradiol action, and provide a biochemical basis for our previous observations of multiphasic effect of progesterone on gonadotropin secretion.

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“…similar to effects of progesterone (104). The role(s) of 5a-DHP and AR in regulation of ovine endometrial physiology remains unknown.…”

Section: Pregnancy Recognition In Ruminants 225mentioning

confidence: 99%

-Interferon: Pregnancy Recognition Signal in Ruminants

Spencer¹,

Ott²,

Bazer³

1996

Experimental Biology and Medicine

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Inhibitory Effect of 5-Alpha-Dihydroprogesterone on Nuclear Estrogen Receptor Binding of the Anterior Pituitary and Uterus in the Rat

Cited by 7 publications

References 28 publications

Changes in Rat Uterine Estrogen Receptor Messenger Ribonucleic Acid Levels during Estrogen- and Progesterone-Induced Estrogen Receptor Depletion and Subsequent Replenishment1

Changes in Rat Uterine Estrogen Receptor Messenger Ribonucleic Acid Levels during Estrogen- and Progesterone-Induced Estrogen Receptor Depletion and Subsequent Replenishment1

Inhibitory Effect of Progesterone on Occupied Estrogen Receptors of Anterior Pituitary and Uterus in Adult Rats

-Interferon: Pregnancy Recognition Signal in Ruminants

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