Inhibitory Effect of Alginic Acids on Hyaluronidase and on Histamine Release from Mast Cells

Asada, Masahiro; Sugie, Makiko; Inoue, Mami; Nakagomi, Kazuya; Hongō, Seiji; Murata, Katsumi; Irie, Shinji; Takeuchi, Toshio; Tomizuka, Noboru; Oka, Syuichi

doi:10.1271/bbb.61.1030

Cited by 76 publications

(44 citation statements)

References 7 publications

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“…Previous reports show that the action of activated hyaluronidase can be involved in the allergic inflammation in line with the histamine release from mast cells (Asada et al, 1997;Sawabe et al, 1992). Hyaluronidase inhibitory activity has been, therefore, considered a model for screening anti-inflammatory materials and/or compounds from natural sources (Asada et al, 1997;Kakegawa et al, 1988;Sawabe et al, 1992).…”

Section: Introductionmentioning

confidence: 99%

Inhibitory effects of the ethyl acetate extract from bulbs ofScilla scilloideson lipoxygenase and hyaluronidase activities

Nishida

Sugahara

Wada

et al. 2014

Pharmaceutical Biology

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Context: Scilla scilloides Druce (Liliaceae) is a folk medicine to treat dermal inflammation; however, the medicinal properties of this plant have not been completely established. Objective: The current study investigates the potent anti-inflammatory effects of S. scilloides bulbs for its traditional usage using lipoxygenase and hyaluronidase as the inflammation model. To gain insight into the active constituents, nine homoisoflavones (1-9) were subsequently tested. Materials and methods: Lipoxygenase and hyaluronidase inhibition of ethyl acetate extract from the bulbs of this plant within 2000 mg/mL or homoisoflavones within 1000 mM were determined by colorimetric methods. RAW264.7 cells were incubated with 10 or 50 mM homoisoflavones plus lipopolysaccharide (LPS) for 24 h. The culture media were collected and analyzed for determination of the nitric oxide (NO) level by the colorimetric Griess method to measure the extent of inflammation. Results: The extract exhibited inhibitory effects on lipoxygenase and hyaluronidase activities with IC 50 values 31.5 and 169 mg/mL, respectively. Among the nine homoisoflavones tested, four (1 and 3-5) resulted in 79.3-97.9% higher lipoxygenase inhibition than 6.7-32.7% of the others at 500 mM. Calculated IC 50 values indicated 5 as the compound responsible for strong lipoxygenase inhibition with 15.8 mM as the IC 50 value. In the hyaluronidase assay, all homoisoflavones tested at 1000 mM demonstrated 16.2-58.0% inhibition. Incubating the cells in the presence of all nine homoisoflavones tested at 50 mM significantly suppressed the NO production, downward to 1.5-66.0%, in the LPS-activated macrophage cells as a model. Discussion and conclusion: These results may indicate a potential role of S. scilloides for anti-inflammatory purposes.

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Section: Introductionmentioning

confidence: 99%

Inhibitory effects of the ethyl acetate extract from bulbs ofScilla scilloideson lipoxygenase and hyaluronidase activities

Nishida

Sugahara

Wada

et al. 2014

Pharmaceutical Biology

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“…Asada et al (1997) reported the amount of uronic acid in the polysaccharide is important for its inhibitory activity on hyaluronidase, and the inhibition of hyaluronidase by alginic acid was the result of substrate competition. The purified preparation from sporophyll of U. pinnatifida had glucuronic acid as an uronic acid, but the amount was small (2.0%).…”

Section: Discussionmentioning

confidence: 99%

“…Asada et al (1999) reported that the glycoprotein obtained from the hot water extract of perilla leaves showed hyaluronidase-inhibitory activity. Alginic acid which exists in the extracellular matrix and the cell membrane of brown algae was also reported to possess this activity (Asada et al, 1997). In view of the important role of hyaluronidase in inflammation and malignant invasion, it is very useful to find hyaluronidase-inhibitory activities in a natural resource, seaweed.…”

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confidence: 99%

Hyaluronidase-Inhibiting Polysaccharide Isolated and Purified from Hot Water Extract of Sporophyll of Undaria pinnatifida

Katšube

Yamasaki

Iwamoto

et al. 2003

FSTR

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Polysaccharide showing inhibitory activity against hyaluronidase, which is known to be related to inflammation and tumor metastasis, was purified from the sporophyll of Undaria pinnatifida by fractionated extraction and column chromatography. On the basis of chemical analyses, the purified compound was found to be a kind of sulfated polysaccharide. The molar ratio of sugars and sulfuric acid in the purified compound was estimated to be L-fucose : D-galactose : D-glucuronic acids : sulfuric acid‫0.1؍‬ : 1.0 : 0.04 : 5.2. The number-average molecular weight of the sulfated polysaccharide was estimated to be 63,000 by high-performance liquid chromatography. This polysaccharide inhibited hyaluronidase activity (IC 50 ‫0.31؍‬ g/ml) in a dose-dependent manner.

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“…9) The HD inhibitory activity was measured by the method of Asada et al 10) FBS, buckwheat green juice, and filter-sterilized FBS were each powdered by freeze-drying and prepared at 100 mg/mL to be tested.…”

Section: Functional Compounds In Fermented Buckwheat Sproutsmentioning

confidence: 99%

Functional Compounds in Fermented Buckwheat Sprouts

Maejima

Nakatsugawa

Ichida

et al. 2011

Bioscience, Biotechnology, and Biochemistry

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Inhibitory Effect of Alginic Acids on Hyaluronidase and on Histamine Release from Mast Cells

Cited by 76 publications

References 7 publications

Inhibitory effects of the ethyl acetate extract from bulbs ofScilla scilloideson lipoxygenase and hyaluronidase activities

Inhibitory effects of the ethyl acetate extract from bulbs ofScilla scilloideson lipoxygenase and hyaluronidase activities

Hyaluronidase-Inhibiting Polysaccharide Isolated and Purified from Hot Water Extract of Sporophyll of Undaria pinnatifida

Functional Compounds in Fermented Buckwheat Sprouts

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