Hyaluronidase-Inhibiting Polysaccharide Isolated and Purified from Hot Water Extract of Sporophyll of Undaria pinnatifida

Katšube, Takao; Yamasaki, Yukikazu; Iwamoto, M.; Oka, Syuichi

doi:10.3136/fstr.9.25

Cited by 21 publications

(19 citation statements)

References 20 publications

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“…Katsube et al [129] isolated a polysaccharide from hot water extract of sporophyll of Undaria pinnatifida. The isolated polysaccharide was found to contain sulfuric acid and sugars such as L-fucose, D-galactose, and D-glucuronic acids.…”

Section: Polymers/polysaccharides/ Sulfated Oligosaccharidesmentioning

confidence: 99%

Hyaluronidase Inhibitors: A Biological and Therapeutic Perspective

Girish¹,

Kemparaju²,

Nagaraju

et al. 2009

CMC

139

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The hyaluronidases (HAases) are a group of less extensively studied glycosidases distributed throughout the animal kingdom and are popularly known as 'spreading factors'. In recent years, HAases received much attention due to their ability to abruptly alter the hyaluronic acid (HA) homeostasis. HAases preferentially degrade HA, which is a megadalton acidic structural polysaccharide found exclusively in the extracellular matrix (ECM) of animals. The HA-HAase system has been suggested to participate in many pathophysiological conditions. The HA degradation in ECM, crack down the structural integrity with an eventual increased tissue permeability that is attributed for the spreading property. The spreading property has been widely accepted in functions including envenomation, acrosomal reaction/ovum fertilization, cancer progression, microbial pathogenesis such as wound infections, pneumonia, and other sepses like, bacteremia and meningitis. HA fragmentation has dual effects; generation of a wide molecular range bioactive oligosaccharides of angiogenic, pro-inflammatory, and immunostimulatory properties; and impairment in the reservoir capacity of ECM that holds metal ions, growth factors, cytokines and various enzymes for signal transduction. Hence, inhibition of HA degradation appears critical and imperative in HAase mediated pathological conditions. HAase inhibitors are thus potent regulators that maintain HA homeostasis and they might serve as anti-inflammatory, anti-aging, anti-microbial, anticancer and anti-venom/toxin and contraceptive agents. In addition, HAase inhibitors may serve as tools to understand several unexplained and complex functions of HAases in HA metabolism. Therefore, this review is expected to provide an integrated update as of 2008 on the HAase inhibitors and their possible role as therapeutics in the management of a wide range of pathological conditions.

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Section: Polymers/polysaccharides/ Sulfated Oligosaccharidesmentioning

confidence: 99%

Hyaluronidase Inhibitors: A Biological and Therapeutic Perspective

Girish¹,

Kemparaju²,

Nagaraju

et al. 2009

CMC

139

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“…Fractionation of the crude extract yielded fractions differing in uronic acid and sulfate ester content and in anticoagulant and lipoprotein clearance activity. More recently, Katsube et al (2003) reported isolation and purification of a hyaluronidase-inhibiting galactofucan sulfate from the sporophyll of U. pinnatifida, Lee et al (2004) described the characterisation and antiviral activity of a highly purified, low molecular weight galactofucan sulfate, also derived from the sporophyll and Thompson and Drager (2004) described the antiviral activity of an extract from blade plus sporophyll. In this paper we describe extraction and fractionation of galactofucan sulfates from U. pinnatifida (blade plus sporophyll), characterisation of the fractions and determination of their antiviral activities against herpes viruses HSV-1, HSV-2 and HCMV.…”

Section: Introductionmentioning

confidence: 98%

Structure and Antiviral Activity of the Galactofucan Sulfates Extracted from Undaria Pinnatifida (Phaeophyta)

Hemmingson¹,

Falshaw²,

Furneaux³

et al. 2006

J Appl Phycol

151

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The galactofucan sulfate extract (GFS) obtained from the brown seaweed Undaria pinnatifida by extraction with dilute acid is a potent inhibitor of the herpes viruses HSV-1, HSV-2 and HCMV, with IC 50 values determined in vitro of 1.1, 0.2 and 0.5 μgmL −1 , respectively. Fractionation of GFS by anion exchange chromatography gave three fractions which differed in their uronic acid and sulfate contents and in their antiviral activity, as well as in having somewhat reduced molecular weights compared to GFS. The low uronic acid/high sulfate fraction (F2M), obtained in 63% yield, had similar molar proportions of galactopyranosyl and fucopyranosyl residues, little associated protein and was equipotent with GFS (IC 50 values of 1.1, 0.1 and 0.5 μgmL −1 , respectively). The high uronic acid/low sulfate fraction (F1M), obtained in 18% yield, had a much lower proportion of galactopyranosyl residues and was less active (IC 50 values of 4.6, 1.0 and 4.0 μgmL −1 , respectively). The minor low uronic acid/high sulfate fraction (F4M) had a significant amount of associated protein and was also less active (IC 50 = 3.1, 1.0 and 2.0 μgmL −1 , respectively). The structure of the major fraction (F2M) was shown to be complex by glycosyl linkage analysis before and after solvolytic desulfation, with many component sugar residues being identified, although 3-linked fucopyranosyl 2,4-disulfate residues were a prominent feature.

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“…As for marine organisms, the sulfated polysaccharide fucoidan 10 and other polysaccharides 11,12 derived from certain seaweeds also have anti‐allergic effects. There are reports of other seaweed compounds besides polysaccharides that may have anti‐allergic effects.…”

Section: Introductionmentioning

confidence: 99%

Inhibitory effects of seaweeds on histamine release from rat basophile leukemia cells (RBL-2H3)

Sugiura

Takeuchi²,

Kakinuma

et al. 2006

Fisheries Sci

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The effects of 80% methanol extracts from frozen samples of 41 macroalgae and one sea grass collected in the Ise-Shima region of Japan were investigated on histamine release from rat basophile leukemia cells (RBL-2H3) sensitized with antidinitrophenyl (DNP) IgE and stimulated with DNP-BSA. Of the 21 brown algae, five green and 15 red algae, and one sea grass tested, only extracts from seven brown algae suppressed histamine release from RBL cells, as determined by highperformance liquid chromatography (HPLC) analysis. When the cytotoxic effects of the seven brown algal extracts were investigated by Trypan blue staining, only Eisenia arborea and Sargassum thunbergii did not show cytotoxic effects. Therefore, we conclude that E. arborea and S. thunbergii may contain compounds that have antiallergic effects without inducing cell death.KEY WORDS: brown algae, cytotoxic effect, histamine release, RBL-2H3 cells.

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Hyaluronidase-Inhibiting Polysaccharide Isolated and Purified from Hot Water Extract of Sporophyll of Undaria pinnatifida

Cited by 21 publications

References 20 publications

Hyaluronidase Inhibitors: A Biological and Therapeutic Perspective

Hyaluronidase Inhibitors: A Biological and Therapeutic Perspective

Structure and Antiviral Activity of the Galactofucan Sulfates Extracted from Undaria Pinnatifida (Phaeophyta)

Inhibitory effects of seaweeds on histamine release from rat basophile leukemia cells (RBL-2H3)

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