Inhibitory effects of seaweeds on histamine release from rat basophile leukemia cells (RBL-2H3)

Abstract

The effects of 80% methanol extracts from frozen samples of 41 macroalgae and one sea grass collected in the Ise-Shima region of Japan were investigated on histamine release from rat basophile leukemia cells (RBL-2H3) sensitized with antidinitrophenyl (DNP) IgE and stimulated with DNP-BSA. Of the 21 brown algae, five green and 15 red algae, and one sea grass tested, only extracts from seven brown algae suppressed histamine release from RBL cells, as determined by highperformance liquid chromatography (HPLC) analysis. When the cytotoxic effects of the seven brown algal extracts were investigated by Trypan blue staining, only Eisenia arborea and Sargassum thunbergii did not show cytotoxic effects. Therefore, we conclude that E. arborea and S. thunbergii may contain compounds that have antiallergic effects without inducing cell death.KEY WORDS: brown algae, cytotoxic effect, histamine release, RBL-2H3 cells.

Investigation of the Antiasthmatic Properties of Ethanol Extract of <i>Callophyllis japonica</i> in Mice

“…Our study has shown that the total phenolic content was high in the EtOH extract of C. japonica. This finding of high phenolic and low carbohydrate content in red algae is consistent with previously published results for brown algae [27].…”

The inhibitory effects of components from a brown alga, Eisenia arborea, on degranulation of mast cells and eicosanoid synthesis

“…These papers reported the anti-allergic and anti-inflammatory effects of polysaccharides (Asada et al, 1997;Maruyama et al, 2005), fatty acids (Ishihara et al, 1998) and phlorotannins (Li et al, 2008;Shibata et al, 2003;Shin et al, 2006). During our studies on the biological activities of algae, we found that an 80% methanol extract of Eisenia arborea (Phaeophyta) has an anti-allergic effect (Sugiura et al, 2006b) and that a dry powder of the alga shows a strong anti-allergic effect on Brown Norway rats, a model strain (Sugiura et al, 2008a(Sugiura et al, , 2008b. The active compounds were identified as six phlorotannins (Fig.…”

“…RBL-2H3 cells (Bursumian et al, 1981) provided by the Japanese Collection of Research Bioresources (JCRB0023, Ibaraki, Osaka, Japan) were cultured in Eagle's Modified Essential Medium (EMEM, MP Biomedicals Inc., Illkirch, France) as described in a previous report (Sugiura et al, 2006b). The cells were transferred every 4 or 5 days by trypsinization with a medium change between the transfers until they attained confluence.…”

Arousal Inhibitory Effect of Phlorotannins on Caffeine in Pentobarbital-Induced Mice

“…Their sensory characteristics would be suitable as an additive to coffee. In addition, phlorotannins have various biological activities, such as antioxidative (Zou et al, 2008), anti-inflammatory , antibacterial , and antiallergic (Sugiura et al, 2006) effects. Therefore, HP-PRT may be a good additive for the preparation of coffee products without an arousal effect.…”