Inhibitory Effect of Alpha-Mangostin to Dengue Virus Replication and Cytokines Expression in Human Peripheral Blood Mononuclear Cells

Sugiyanto, Zaenal; Yohan, Benediktus; Hadisaputro, Soeharyo; Dharmana, Edi; Suharti, Catharina; Winarto, Winarto; Djamiatun, Kis; Rahmi, Fifin Luthfia; Sasmono, R. Tedjo

doi:10.1007/s13659-019-00218-z

Cited by 20 publications

(18 citation statements)

References 28 publications

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“…Several studies reported that it possesses a wide range of biological activities including anti-inflammatory, anti-allergic, antiviral, antibacterial, antifungal, anti-parasitic, antioxidant, and anti-cancer properties [ 27 , 28 ]. Earlier studies have also shown that α-Mangostin can inhibit DENV in peripheral blood mononuclear cells and hepatic cell lines and down-regulate the expression of pro-inflammatory cytokines [ 29 , 30 ]. These studies have shown the effect of α-Mangostin post-infection.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Antiviral Activity of α-Mangostin against Dengue Virus Serotype-2 (DENV-2)

et al. 2021

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Dengue virus (DENV), a member of the family Flaviviridae, is a threat for global health as it infects more than 100 million people yearly. Approved antiviral therapies or vaccines for the treatment or prevention of DENV infections are not available. In the present study, natural compounds were screened for their antiviral activity against DENV by in vitro cell line-based assay. α-Mangostin, a xanthanoid, was observed to exert antiviral activity against DENV-2 under pre-, co- and post-treatment testing conditions. The antiviral activity was determined by foci forming unit (FFU) assay, quantitative RT-PCR and cell-based immunofluorescence assay (IFA). A complete inhibition of DENV-2 was observed at 8 µM under the co-treatment condition. The possible inhibitory mechanism of α-Mangostin was also determined by docking studies. The molecular docking experiments indicate that α-Mangostin can interact with multiple DENV protein targets such as the NS5 methyltransferase, NS2B-NS3 protease and the glycoprotein E. The in vitro and in silico findings suggest that α-Mangostin possesses the ability to suppress DENV-2 production at different stages of its replication cycle and might act as a prophylactic/therapeutic agent against DENV-2.

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Section: Discussionmentioning

confidence: 99%

In Vitro Antiviral Activity of α-Mangostin against Dengue Virus Serotype-2 (DENV-2)

et al. 2021

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“…The ethanolic extract of mangosteen fruit is considered as a potent inhibitor of HIV‐1 protease activity and the antiviral property of this fruit is due to the presence of purified molecules like α‐mangostin and γ‐mangostin (Chen et al., 1996 ). The administration of α‐mangostin (obtained from the pericarps of mangosteen) to dengue patients during the acute phase of illness may decrease infection severity by activating the host’s immune response through mechanism of interfering DENV NS5 protein activity which is essential for DENV replication and reducing transcriptional responses of cytokines (Sugiyanto et al., 2019 ).…”

Section: Introductionmentioning

confidence: 99%

“…The effect of α‐mangostin against the DENV infection and post‐infection in PBMCs, TNF‐α and IFN‐γ cytokines revealed that increasing concentration of α‐mangostin inhibits the viral replication by more than 50% with the IC 50 of 5.47 and 5.77 μM for 24 and 48 hr of post‐treatments, respectively (Sugiyanto et al., 2019 ). The α‐mangostin inhibits DENV production in cultured hepatocellular carcinoma HepG2 and Huh‐7 cells, and cytokine/chemokine expression in HepG2 cells (Tarasuk et al., 2017 ).…”

Section: Introductionmentioning

confidence: 99%

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Therapeutic potential of phytoconstituents of edible fruits in combating emerging viral infections

et al. 2021

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Plant‐derived bioactive molecules display potential antiviral activity against various viral targets including mode of viral entry and its replication in host cells. Considering the challenges and search for antiviral agents, this review provides substantiated data on chemical constituents of edible fruits with promising antiviral activity. The bioactive constituents like naringenin, mangiferin, α‐mangostin, geraniin, punicalagin, and lectins of edible fruits exhibit antiviral effect by inhibiting viral replication against IFV, DENV, polio, CHIKV, Zika, HIV, HSV, HBV, HCV, and SARS‐CoV. The significance of edible fruit phytochemicals to block the virulence of various deadly viruses through their inhibitory action against the entry and replication of viral genetic makeup and proteins are discussed. In view of the antiviral property of active constituents of edible fruits which can strengthen the immune system and reduce oxidative stress, they are suggested to be diet supplements to combat various viral diseases including COVID‐19. Practical applications Considering the increasing threat of COVID‐19, it is suggested to examine the therapeutic efficacy of existing antiviral molecules of edible fruits which may provide prophylactic and adjuvant therapy with their potential antioxidant, anti‐inflammatory, and immune‐modulatory effects. Several active molecules like geraniin, naringenin, (2R,4R)‐1,2,4‐trihydroxyheptadec‐16‐one, betacyanins, mangiferin, punicalagin, isomangiferin, procyanidin B2, quercetin, marmelide, jacalin lectin, banana lectin, and α‐mangostin isolated from various edible fruits have showed promising antiviral properties against different pathogenic viruses. Especially flavonoid compounds extracted from edible fruits possess potential antiviral activity against a wide array of viruses like HIV‐1, HSV‐1 and 2, HCV, INF, dengue, yellow fever, NSV, and Zika virus infection. Hence taking such fruits or edible fruits and their constituents/compounds as dietary supplements could deliver adequate plasma levels in the body to optimize the cell and tissue levels and could lead to possible benefits for the preventive measures for this pandemic COVID‐19 situation.

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“…The antioxidant and neuroprotective effects of α-mangostin on 6-hydroxydopamine-induced toxicity in SH-SY5Y cells as an in vitro model for Parkinson's disease have been demonstrated. [22] Moreover, α-mangostin has been found to inhibit the production of both dengue virus and cytokines/chemokines, [23] act as antiviral and anti-inflammatory agent in the treatment of dengue fever, [24] have a biological role via the TAK1-NF-κB pathway against inflammation, [25] induce the necrotic death of BT474 breast cancer cells through autophagy and inflammation, [26] and exhibit inhibitory effects on cholangiocarcinoma cells and allografts. [27] The α-mangostin showed apoptotic effect on human breast cancer cells via inhibiting activity of fatty acid synthase (FAS), [28] abrogates anoikis resistance in human hepatocellular carcinoma cells [19] and migration and invasion of A549 lung cancer cells.…”

Section: Introductionmentioning

confidence: 99%

Existence of α‐mangostin conformers and effects of aprotic and protic solvents on their equilibria, UV–Vis spectra, and chemical descriptors: Density functional theory and time‐dependent density functional theory study

Kaewsud

Ruangpornvisuti

2020

J of Physical Organic Chem

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The physicochemical, thermodynamic, spectroscopic, and electronic properties of α‐mangostin dye (1,3,6‐trihydroxy‐7‐methoxy‐2,8‐bis(3‐methyl‐2‐buten‐1‐yl)‐9H‐xanthen‐9‐one) were investigated. Five conformers of the α‐mangostin dye were obtained using three different polarizable continuum model (PCM)/density functional theory (DFT) methods: wB97XD/6‐31+G(d,p), M06‐2X/6‐31+G(d,p), and B3LYP/6‐31+G(d,p). The species distribution of the five conformers in acetonitrile, water, and the gas phase was derived from the Gibbs free energy changes of their interconversion equilibria. Three dominant conformers (Conformers 2, 3, and 4) were found as coexisting species in solvents. Chemical descriptors of the three dominant conformers of the α‐mangostin in acetonitrile and water were obtained. Simulated UV–Vis spectra of the existing conformers and their mixtures in acetonitrile and water derived from the PCM/TD‐DFT calculations were in good agreement with experimental results. Because the three dominant conformers of α‐mangostin absorb light in the UV region, the application of α‐mangostin dye for UV protection is suggested. The performance of the three dominant α‐mangostin conformers in dye sensitized solar cells was also investigated.

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Inhibitory Effect of Alpha-Mangostin to Dengue Virus Replication and Cytokines Expression in Human Peripheral Blood Mononuclear Cells

Cited by 20 publications

References 28 publications

In Vitro Antiviral Activity of α-Mangostin against Dengue Virus Serotype-2 (DENV-2)

In Vitro Antiviral Activity of α-Mangostin against Dengue Virus Serotype-2 (DENV-2)

Therapeutic potential of phytoconstituents of edible fruits in combating emerging viral infections

Existence of α‐mangostin conformers and effects of aprotic and protic solvents on their equilibria, UV–Vis spectra, and chemical descriptors: Density functional theory and time‐dependent density functional theory study

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