Inhibitory Effect of Aqueous Extract of Stem Bark of Cissus populnea on Ferrous Sulphate- and Sodium Nitroprusside-Induced Oxidative Stress in Rat’s Testes In Vitro

Abstract

Cissus populnea are plants associated with a myriad of medicinal uses in different parts of the world and are good sources of carotenoids, triterpenoids, and ascorbic acid. The antioxidant properties and inhibitory effect of water extractible phytochemicals from stem bark of C. populnea on FeSO4 and sodium nitroprusside- (SNP-) induced lipid peroxidation in rat testes were investigated in vitro. The results revealed that the extract was able to scavenge DPPH radical, chelate Fe2+ and also had a high reducing power. Furthermore, the incubation of the testes tissue homogenate in the presence of FeSO4 and SNP, respectively, caused a significant increase in the malondialdehyde (MDA) contents of the testes. However, the aqueous extract of the stem bark of C. populnea caused a significant decrease in the MDA contents of both Fe2+ (EC50 = 0.027 mg/mL) and SNP- (EC50 = 0.22 mg/mL) induced lipid peroxidation in the rat testes homogenates in a dose-dependent manner. The water extractible phytochemicals from C. populnea protect the testes from oxidative stress and this could be attributed to their high antioxidant activity: DPPH-scavenging ability, Fe2+-chelating and -reducing power. Therefore, oxidatively stress in testes could be potentially managed/prevented by this plant.

Effect of Alkaloid Extract from African Jointfir (Gnetum africanum) Leaves on Manganese‐Induced Toxicity in Drosophila melanogaster

“…Plant-based bioactive phytochemicals such as polyphenols and alkaloids have shown promising neuroprotective potentials in both in vitro [15] and in vivo rat [16, 17] and drosophila models [18, 19]. Green leafy vegetables form a major constituent of local diets and are the major sources of plant bioactive phytochemicals.…”

Manniophyton fulvum extract attenuates sodium nitroprusside induced oxidative stress in wistar rats

“…2 [17][18][19][20] The metabolism of the drug has been widely studied both in experimental animals and humans as classical inductor of oxidative stress. 21,22 From the study, the group administered with SNP only showed significant increase in MDA concentration while rhodanese and GSH activity showed significant decrease compared to the control which may be attributed to the decomposition of SNP. The decrease observed in rhodanese activity and GSH may be attributed to participation in cyanide detoxification and antioxidant activity.…”

Cannabis sativa L. (var. indica) Exhibits Hepatoprotective Effects by Modulating Hepatic Lipid Profile and Mitigating Gluconeogenesis and Cholinergic Dysfunction in Oxidative Hepatic Injury

“…Oxidative injury was induced in vitro using earlier established protocols with slight modifications (Akomolafe et al, 2013;Kizil et al, 2010). Briefly, 100 µl of the hepatic tissue supernatant was incubated with 15-240 μg/ml each of hexane, DCM, and ethanol extracts in the presence of 0.1 mM of FeSO 4 at 37 °C for 30 min.…”