Inhibitory Effect of Black Radish (Raphanus sativus L. var. niger) Extracts on Lipopolysaccharide-Induced Inflammatory Response in the Mouse Monocyte/Macrophage-Like Cell Line RAW 264.7

Jeon, Hyungsik; Yang, Dan; Lee, Nam Ho; Ahn, Meejung; Kim, Gi-Ok

doi:10.3746/pnf.2020.25.4.408

Cited by 10 publications

(1 citation statement)

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“…In the case of both radish leaves and roots, stronger inhibition of So far, radish has been subjected to many studies on the effects of cytokines involved in inflammatory processes in many cell lines. Jeon et al showed that black radish extract significantly inhibited the increased production of NO, TNF-α, IL-6, and IL-1β by RAW 264.7 cells [49]. Moreover, the chloroform fraction of R. sativus L. leaves inhibited the expression of iNOS and COX-2 in RAW264.7 macrophages, thereby reducing inflammation [50].…”

Section: Assessment Of Antibacterial Activitymentioning

confidence: 99%

Comparison of Anti-Inflammatory and Antibacterial Properties of Raphanus sativus L. Leaf and Root Kombucha-Fermented Extracts

Ziemlewska,

Zagórska-Dziok,

Mokrzyńska

et al. 2024

IJMS

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In the cosmetics industry, the extract from Raphanus sativus L. is fermented using specific starter cultures. These cosmetic ingredients act as preservatives and skin conditioners. Kombucha is traditionally made by fermenting sweetened tea using symbiotic cultures of bacteria and yeast and is used in cosmetic products. The aim of this study was to evaluate the cosmetic properties of radish leaf and root extract fermented with the SCOBY. Both unfermented water extracts and extracts after 7, 14, and 21 days of fermentation were evaluated. The analysis of secondary plant metabolites by UPLC-MS showed higher values for ferments than for extracts. A similar relationship was noted when examining the antioxidant properties using DPPH and ABTS radicals and the protective effect against H2O2-induced oxidative stress in fibroblasts and keratinocytes using the fluorogenic dye H2DCFDA. The results also showed no cytotoxicity to skin cells using Alamar Blue and Neutral Red tests. The ability of the samples to inhibit IL-1β and COX-2 activity in LPS-treated fibroblasts was also demonstrated using ELISA assays. The influence of extracts and ferments on bacterial strains involved in inflammatory processes of skin diseases was also assessed. Additionally, application tests were carried out, which showed a positive effect of extracts and ferments on TEWL and skin hydration using a TEWAmeter and corneometer probe. The results obtained depended on the concentration used and the fermentation time.

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Section: Assessment Of Antibacterial Activitymentioning

confidence: 99%