Inhibitory Effect of Dauricine on Inflammatory Process Following Focal Cerebral Ischemia/Reperfusion in Rats

Abstract

Our previous experimental studies showed that dauricine could protect the brain from ischemic damage, but the underlying mechanisms were unknown. In this study, we investigated the effect of dauricine on the changes of the inflammation process induced by ischemia/reperfusion (I/R). After I/R, the enzyme activity of MPO, the expression of ICAM-1 and the transcription of IL-1beta and TNF-alpha mRNA were all significantly increased (p < 0.01). And after treatment with dauricine, they were all significantly reduced compared to the vehicle-treated I/R group (p < 0.05 or p < 0.01). These results suggest that dauricin attenuates the inflammation process induced by I/R. The neuroprotective effect of dauricine may partly due to the inhibition acute inflammation induced by I/R.

Dauricine inhibits insulin-like growth factor-I-induced hypoxia inducible factor 1α protein accumulation and vascular endothelial growth factor expression in human breast cancer cells

“…Dauricine (Dau; Figure 1), a bisbenzylisoquinoline alkaloid isolated from the rhizome of Menispermum dauricum DC, has been found to have antiarrhythmic and antiinflammatory effects [1] . Recently, several studies have demonstrated that Dau has potent anticancer activities, including inhibition of growth [2] , reduction of drug resistance, and promotion of apoptosis in various tumor cells.…”

Protective effects of doxycycline in ischemia/reperfusion injury on kidney

“…Although we find little literature about the effects of Dc on ischemia/reperfused rat kidneys, YEH et al had reported that Dc has protective effects against apoptosis in mouse testes (30). It is well known that I/R injury increases the interleukin 1-beta (IL-1β) levels in several tissues (13,29). It has been reported that Dc can suppress the expression of IL-1β on cultured conjunctival epithelial cells (28) and that Dc inhibits the matrix metalloproteinases (MMPs) suggesting that it has inhibitory effects on various MMPs (8).…”

Neuroprotective Effect of Dauricine after Transient Middle Cerebral Artery Occlusion in Rats: Involvement of Bcl-2 Family Proteins

“…Transient focal ischemia was made by intraluminal middle cerebral artery (MCA) blockade with a 3-0 nylon suture, as described previously (Yang et al, 2007). The animals were kept on an isothermal pad.…”

“…Previous studies in our laboratory and others have observed that it reduces energy depletion and oxidative stress (Li and Gong, 2004;He et al, 1997). Recently, we demonstrated that multiple pre-ischemic doses of dauricine reduced infract size Yang et al, 2005) by inhibiting the release of cytochrome c and the activation of caspase 9 and caspase 3 (Li et al, 2007a). Furthermore, dauricine suppressed the decrease of mitochondrial membrane potential ( ψ m ) induced by hypoxia and hypoglycaemia (Li et al, 2007b).…”