Inhibitory effect of microvesicles collected from stored blood on platelet aggregation

Daveloose, Denis; Wolf, Claude; Jeanguillaume, C.; Leterrier, F; Fabre, Gérard; Béréziat, G.

doi:10.1016/0049-3848(81)90321-2

Cited by 5 publications

(1 citation statement)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“… 20 Different from microvesicles (100 nm to 1 μm) separated from the membrane directly, exosome is mainly formed through the endosomal pathway. 21 – 24 The biogenesis of exosome begins with inward invagination of the cell membrane, causing the incorporation of membrane proteins in the early endosome. As the early endosome matures and develops into the late endosome, it undergoes inward budding resulting in the formation of intraluminal vesicles.…”

Section: Introductionmentioning

confidence: 99%

Advances of exosome in the development of ovarian cancer and its diagnostic and therapeutic prospect

Shen

Zhu

Jing

et al. 2018

OTT

View full text Add to dashboard Cite

Ovarian cancer is the leading cause of female gynecological cancer mortality. Most patients with ovarian cancer are diagnosed with advanced stage because of lack of early symptoms, physical signs, and sensitive tumor biomarkers. The standard treatment includes cytoreductive surgery and platinum-based chemotherapy (usually platinum combined with paclitaxel). Despite that postoperative adjuvant chemotherapy prolongs survival time, most patients go through relapse within 6–12 months after the treatment. Thus, elucidating the molecular mechanism in cancer development is essential to promote early diagnosis and novel treatments. The role of exosome has been highlighted in multiple research fields in recent years. Exosome has been described as nano-sized vesicle secreted by multiple mammalian cell types, carrying cargos like proteins, miRNAs, mRNAs, and lipids. It participates in the formation of tumor microenvironment and the development of tumorigenesis and drug resistance in ovarian cancer. Meanwhile, it may also play a pivotal role in diagnosis, efficacy evaluation, and prognosis. Besides, studies show that exosome and its processed products have promising value in ovarian cancer treatment. The aim of the current review is to describe the characteristics of exosome in ovarian cancer, especially focusing on its role in immune modulation and drug resistance, hoping to provide new information on its implications in cancer diagnosis and treatment.

show abstract

Section: Introductionmentioning

confidence: 99%

Advances of exosome in the development of ovarian cancer and its diagnostic and therapeutic prospect

Shen

Zhu

Jing

et al. 2018

OTT

View full text Add to dashboard Cite

show abstract

Exosomes as a Potential Tool for a Specific Delivery of Functional Molecules

Nazarenko

Rupp

Altevogt

2013

Methods in Molecular Biology

View full text Add to dashboard Cite

Extracellular membrane vesicles derived from the endosomal compartments and released by the fusion of the multivesicular bodies with the cell membrane are referred as exosomes (Exo) [Van Niel et al., J Biochem 140:13-21, 2006]. They function as mediators of intercellular communication and are employed by the organism in the regulation of systemic and local processes. Meantime, Exo are recognized as an indispensable entity of physiological fluids [Caby et al., Int Immunol 17:879-887, 2005; Lasser et al., J Transl Med 9:9, 2011; Lasser et al., Am J Rhinol Allergy 25:89-93, 2011]. Exo and other types of extracellular vesicles, e.g., exosome-like vesicles [van Niel et al., Gastroenterology 121:337-349, 2001] and microvesicles (MV) [Daveloose et al., Thromb Res 22:195-201, 1981], contain multiple functional molecules including lipids [Vidal et al., J Cell Physiol 140:455-462, 1989]; proteins [Simpson et al., Expert Rev Proteomics 6:267-283, 2009]; mRNA [Valadi et al., Nat Cell Biol 9:654-659, 2007]; DNA [Waldenstrom et al., PLoS One 7:e34653, 2012]; noncoding RNA, e.g., miRNA [Simpson et al., Expert Rev Proteomics 6:267-283, 2009]; and retrotransposon elements [Balaj et al., Nat Commun 2:180, 2011]. Assessment of the biological functions of Exo showed that they deliver specifically their cargo from the donor to recipient cells. Albeit the molecular mechanisms of this process are not fully understood, approaches for the application of Exo and MV as a tool for a cell-specific delivery of signalling molecules were successfully tested in in vitro and in vivo models [Maguire et al., Mol Ther 20:960-971, 2012]. Ovarian cancer cells release Exo, which bind stroma cells as well as donor cancer cells [Escrevente et al., BMC Cancer 11:108, 2011]. Here we describe an experimental approach for the assessment of Exo interaction and uptake by target cells. Methods for the isolation and purification of Exo from cell culture supernatants are included. To allow visualization of vesicle uptake, labelling of Exo with different fluorescent dyes, such as CFSE, PKH, DHPE, and DiOC18, is presented. Finally, we explain qualitative and quantitative analysis of Exo uptake by immunofluorescence and flow cytometry, respectively.

show abstract

Protein-stimulated enrichment of human platelet membranes in linoleylphosphatidylcholines. Effect upon adenylate cyclase and fluidity

Chambaz

Pépin

Robert

et al. 1983

Biochimica et Biophysica Acta (BBA) - Biomembranes

View full text Add to dashboard Cite

Inhibitory effect of microvesicles collected from stored blood on platelet aggregation

Cited by 5 publications

References 8 publications

Advances of exosome in the development of ovarian cancer and its diagnostic and therapeutic prospect

Advances of exosome in the development of ovarian cancer and its diagnostic and therapeutic prospect

Exosomes as a Potential Tool for a Specific Delivery of Functional Molecules

Protein-stimulated enrichment of human platelet membranes in linoleylphosphatidylcholines. Effect upon adenylate cyclase and fluidity

Contact Info

Product

Resources

About