Inhibitory effect of microwaved thinned nectarine extracts on polyphenol oxidase activity

Redondo, Diego; Venturini, Marı́a Eugenia; Oria, Rosa; Arias, Esther

doi:10.1016/j.foodchem.2015.11.009

Cited by 30 publications

(44 citation statements)

References 37 publications

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“…The activity obtained with the FRAP assay in passion fruit peel (4.4 mg TE g −1 DW) was much lower than that in our thinned samples. Therefore, if all the above authors conclude that the by‐products studied represent a source of antioxidant compounds, it seems clear that thinned fruits must be an important source of interesting compounds that may be used in the food, chemical and pharmaceutical industries as antioxidants or anti‐browning agents …”

Section: Resultsmentioning

confidence: 99%

Thinned stone fruits are a source of polyphenols and antioxidant compounds

et al. 2016

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Section: Resultsmentioning

confidence: 99%

Thinned stone fruits are a source of polyphenols and antioxidant compounds

et al. 2016

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“…Microwaved extracts obtained from by-products of the agro-industry, such as thinned fruits, represent another tool for the prevention of enzymatic browning [95]. Indeed, the microwave treatment induces the formation of Maillard reaction products (MRPs), associated with antioxidant activity.…”

Section: Polyphenol Oxidases (Ppo) and Browningmentioning

confidence: 99%

“…Indeed, the microwave treatment induces the formation of Maillard reaction products (MRPs), associated with antioxidant activity. A solution of 2% extract was reported to inhibit PPOs in minimally processed peaches for eight days of storage [95]. Finally, modified atmosphere packaging with low permeability films is another effective way for extending shelf-life of processed fruit and vegetables, as oxygen is needed to trigger browning.…”

Section: Polyphenol Oxidases (Ppo) and Browningmentioning

confidence: 99%

“…PPO activity was also associated with the chromosome arm 2BL, even though the phenotypic effect in this case resulted to be lower than its homoeologous region on chromosome 2AL [69,70,71,72,73,74,75,76,77,78,79,80,81,82,83,84,85,86,87,88,89,90,91,92,93,94,95,96,97,98,99,100,101,102,103,104,105,106,107,108,109,110,111,112,113,114,115,116,117,118,119,120,121,122]. Finally, a major QTL was detected on chromosome arm 2DL, which explained 23% of phenotypic PPO variation [69,70,71,72,73,74,75,76,77,78,79,80,81,82,83,…”

Section: Genomic and Genetic Aspectsmentioning

confidence: 99%

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Polyphenol Oxidases in Crops: Biochemical, Physiological and Genetic Aspects

Taranto

Pasqualone

Mangini

et al. 2017

IJMS

325

187

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Enzymatic browning is a colour reaction occurring in plants, including cereals, fruit and horticultural crops, due to oxidation during postharvest processing and storage. This has a negative impact on the colour, flavour, nutritional properties and shelf life of food products. Browning is usually caused by polyphenol oxidases (PPOs), following cell damage caused by senescence, wounding and the attack of pests and pathogens. Several studies indicated that PPOs play a role in plant immunity, and emerging evidence suggested that PPOs might also be involved in other physiological processes. Genomic investigations ultimately led to the isolation of PPO homologs in several crops, which will be possibly characterized at the functional level in the near future. Here, focusing on the botanic families of Poaceae and Solanaceae, we provide an overview on available scientific literature on PPOs, resulting in useful information on biochemical, physiological and genetic aspects.

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“…Such browning often accompanies senescence, mechanical damage, and attack by pathogens or herbivores [7,8,9]. The negative effect of PPOs on the appearance and nutritional quality of products has prompted numerous ecological and molecular studies, as has the role of PPOs in plant defense against herbivores and pathogens [10,11,12,13,14,15]. For instance, an inverse correlation has been found between the performance of cotton bollworm ( Helicoverpa armigera (Hübner)), beet armyworm ( Spodoptera exigua (Hübner)), and PPO levels [12,16], and the antisense suppression of potato ( Solanum tuberosum L.) StPPO has been shown to increase susceptibility, and the overexpression of PPO cDNAs has been demonstrated in tomato ( Lycopersicon esculentum L.) to increase resistance to Pseudomonas syringae pv.…”

Section: Introductionmentioning

confidence: 99%

Two New Polyphenol Oxidase Genes of Tea Plant (Camellia sinensis) Respond Differentially to the Regurgitant of Tea Geometrid, Ectropis obliqua

Huang

Jin

Zhang

et al. 2018

IJMS

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Polyphenol oxidases (PPOs) have been reported to play an important role in protecting plants from attacks by herbivores. Though PPO genes in other plants have been extensively studied, research on PPO genes in the tea plant (Camellia sinensis) is lacking. In particular, which members of the PPO gene family elicit the defense response of the tea plant are as yet unknown. Here, two new PPO genes, CsPPO1 and CsPPO2, both of which had high identity with PPOs from other plants, were obtained from tea leaves. The full length of CsPPO1 contained an open reading frame (ORF) of 1740 bp that encoded a protein of 579 amino acids, while CsPPO2 contained an ORF of 1788 bp that encoded a protein of 595 amino acids. The deduced CsPPO1 and CsPPO2 proteins had calculated molecular masses of 64.6 and 65.9 kDa; the isoelectric points were 6.94 and 6.48, respectively. The expression products of recombinant CsPPO1 and CsPPO2 in Escherichia coli were about 91 and 92 kDa, respectively, but the recombinant proteins existed in the form of an inclusion body. Whereas CsPPO1 is highly expressed in stems, CsPPO2 is highly expressed in roots. Further results showed that the expression of CsPPO1 and CsPPO2 was wound- and Ectropis obliqua-induced, and that regurgitant, unlike treatment with wounding plus deionized water, significantly upregulated the transcriptional expression of CsPPO2 but not of CsPPO1. The difference between regurgitant and wounding indicates that CsPPO2 may play a more meaningful defensive role against E. obliqua than CsPPO1. Meanwhile, we found the active component(s) of the regurgitant elicited the expression of CsPPO may contain small molecules (under 3-kDa molecular weight). These conclusions advance the understanding of the biological function of two new PPO genes and show that one of these, CsPPO2, may be a promising gene for engineering tea plants that are resistant to E. obliqua.

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Inhibitory effect of microwaved thinned nectarine extracts on polyphenol oxidase activity

Cited by 30 publications

References 37 publications

Thinned stone fruits are a source of polyphenols and antioxidant compounds

Thinned stone fruits are a source of polyphenols and antioxidant compounds

Polyphenol Oxidases in Crops: Biochemical, Physiological and Genetic Aspects

Two New Polyphenol Oxidase Genes of Tea Plant (Camellia sinensis) Respond Differentially to the Regurgitant of Tea Geometrid, Ectropis obliqua

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