Inhibitory effect of morin on aldolase 2 from Eimeria tenella

Characterization ofArabidopsisaldolases AtFBA4 and AtFBA5; inhibition by morin and interaction with calmodulin

“…Similar to a recent report on the inhibition of E. tenella FBAs by the plant flavonoid morin (17), we found morin to be a potent inhibitor of AtFBA4 and AtFBA5, raising questions about a potential regulatory role for flavonoids in plant glycolysis. Flavonoids participate in diverse physiological processes, including defense against UV radiation, pathogen attacks, low nutrient, and oxidative stress (48, 36).…”

“…Thus, we investigated its effects on AtFBA4 and AtFBA5 activity. Similar to its action on EtFBA2 (17), morin exerted non-competitive inhibition of AtFBA4 and AtFBA5, with Ki values of 80 ±10 and 63 ±4 µM for AtFBA4 and AtFBA5, respectively (Fig. 2E, F).…”

“…The flavonol morin is a specialized plant metabolite and potent inhibitor of protozoan FBAs, and there is considerable interest in using morin as a potential therapeutic agent (17, 18). Thus, we investigated its effects on AtFBA4 and AtFBA5 activity.…”

“…As FBAs play a central role in glycolysis, there is interest in them as potential drug targets in parasites. A recent study identified the plant flavonoid morin (3,5,7,2′,4′-pentahydroxyflavone) as a putative class-I FBA inhibitor of EtFBA2 from the protozoan parasite Eimeria tenella (17). Morin is derived from the common plant flavonoid quercetin, is primarily found in mulberry plants, and possesses various therapeutic properties in animals, including anti-inflammatory, anti-bacterial, and anti-tumor effects (18).…”

“…The latter objective was based on a recent report indicating that orthologs of AtCML11 and AtFBA4 in M. sativa , MsCML10 and MsFBA6, respectively, physically interact and impact MsFBA6 activity and the accumulation of compatible solutes during stress (8). Our third objective was to assess whether morin inhibits plant FBA in vitro activity as has been reported for a protozoan FBA (17).…”