Inhibitory effect of roxithromycin on the local levels of bone-resorbing cytokines in an experimental model of murine osteomyelitis

Yoshii, Takashi; Magara, Shinya; Miyai, Daisuke; Kuroki, Eiji; Nishimura, Hidetaka; Furudoi, Shungo; Komori, Takahide

doi:10.1093/jac/dkf124

Cited by 12 publications

(6 citation statements)

References 6 publications

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“…Bone wax was used to seal the access burr hole and sequential study of the infectious process and establishment of osteomyelitis was observed. Both Sottnik et al (Sottnik et al , 2010) and Yoshii et al (Yoshii et al , 2002a; Yoshii et al , 2002b) utilize a transcortical hole in the proximal part of the tibia with implanted S. aureus seeded suture material as the source of the osteomyelitis. Varoga et al (Varoga et al , 2008; Varoga et al , 2009) modified a rat model initially described by Lucke et al (Lucke et al , 2003b), in which an access hole in the proximal part of the tibia allowed access to the medullary canal for the introduction of an inoculum.…”

Section: Literature Review Of Animal Modelsmentioning

confidence: 99%

A systematic review of animal models for Staphylococcus aureus osteomyelitis

Reizner¹,

Hunter²,

O’Malley³

et al. 2014

eCM

112

106

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Staphylococcus aureus (S. aureus) osteomyelitis is a significant complication for orthopaedic patients undergoing surgery, particularly with fracture fixation and arthroplasty. Given the difficulty in studying S. aureus infections in human subjects, animal models serve an integral role in exploring the pathogenesis of osteomyelitis, and aid in determining the efficacy of prophylactic and therapeutic treatments. Animal models should mimic the clinical scenarios seen in patients as closely as possible to permit the experimental results to be translated to the corresponding clinical care. To help understand existing animal models of S. aureus, we conducted a systematic search of PubMed and Ovid MEDLINE to identify in vivo animal experiments that have investigated the management of S. aureus osteomyelitis in the context of fractures and metallic implants. In this review, experimental studies are categorised by animal species and are further classified by the setting of the infection. Study methods are summarised and the relevant advantages and disadvantages of each species and model are discussed. While no ideal animal model exists, the understanding of a model's strengths and limitations should assist clinicians and researchers to appropriately select an animal model to translate the conclusions to the clinical setting.

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Section: Literature Review Of Animal Modelsmentioning

confidence: 99%

A systematic review of animal models for Staphylococcus aureus osteomyelitis

Reizner¹,

Hunter²,

O’Malley³

et al. 2014

eCM

112

106

View full text Add to dashboard Cite

show abstract

“…The concentration of TNF-␣ in the bone microenvironment in vivo has been estimated to be ∼100-200 ng/g and increase up to 600-1500 ng/g on stimulation with pathogenic agents. (51) In the bone microenvironment, cell sources that are likely candidates for TNF-␣ production include the osteoblastic cells, (40) adipocytes, (52) endothelial cells, (53) T lymphocytes, and the monocytes/macrophages, (54) which have been shown to affect bone resorption in vivo, whereas studies have also indicated the capacity of TNF-␣ to regulate osteoclastic activity in an autocrine fashion in vitro. (55) Importantly, addition of a TNF-␣-neutralizing antibody both in vitro and in vivo significantly blocked the OAB-related osteoclastogenic and the bone resorptive response.…”

Section: Figmentioning

confidence: 99%

Apoptotic Bodies Convey Activity Capable of Initiating Osteoclastogenesis and Localized Bone Destruction

Kogianni

Mann

Noble

2008

Journal of Bone and Mineral Research

190

117

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ABSTRACT:Introduction: Osteocyte apoptosis co-localizes with sites of osteoclastic bone resorption in vivo, but to date, no causal molecular or signaling link has been identified between these two processes. Materials and Methods: Osteocyte apoptotic bodies (OABs) derived from the MLO-Y4 osteocyte-like cell line and primary murine osteocytes and apoptotic bodies (ABs) derived from primary murine osteoblasts were introduced onto the right parietal bone of murine calvariae, and osteoclastic bone resorption was examined 5 days after treatment. In addition, the ability of primary murine and cell line-derived OABs to support osteoclastogenesis was examined in vitro in co-culture with murine bone marrow hematopoietic progenitors in the absence of RANKL or macrophage-colony stimulating factor. Results: For the first time, we show that OABs are capable of initiating de novo osteoclastic bone resorption on quiescent bone surfaces in vivo. Furthermore, the addition of OABs to mononuclear osteoclast precursors (OPs) in vitro resulted in the maintenance of OP cell numbers and an increase in the proportion and activity of TRACP + cells. In contrast, application of ABs from osteoblasts showed no osteoclastogenic activity either in vivo or in vitro. The osteoclastogenic capacity of OABs was shown to be independent of the known osteoclastogenic factor RANKL but dependent on the induction of TNF-␣ production by OP. Conclusions: These data point to a mechanism by which dying osteocytes might target bone destruction through the distribution of OAB-associated signals and give further physiological meaning to the apoptotic process in bone.

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“…The injury can also be contaminated from the insertion of a surgical material into the wound such as a pre-inoculated silk suture (Yoshii, 2002a;Yoshii et al, 2002b), pre-inoculated agarose beads (Marriott et al, 2004;Marriott et al, 2005) or a pre-inoculated collagen sheet (Inzana et al, 2015b).…”

Section: Inoculation Methods and Dosementioning

confidence: 99%

Current osteomyelitis mouse models, a systematic review

Guarch-Pérez¹,

Riool²,

Zaat

2021

eCM

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Osteomyelitis is an inflammatory bone disease caused by an infecting microorganism leading to a gradual bone loss. Due to the difficulty in studying osteomyelitis directly in patients, animal models allow researchers to investigate the pathogenesis of the infection and the development of novel prophylactic, anti-inflammatory and antimicrobial treatment strategies. This review is specifically focused on the in vivo mouse osteomyelitis studies available in literature. Thus, a systematic search on Web of Science and PubMed was conducted using the query “(infection) AND (mice OR mouse OR murine) AND (model OR models) AND (arthroplasty OR fracture OR (internal fixator) OR (internal fixation OR prosthesis OR implant OR osteomyelitis)”. After critical assessment of the studies according to the inclusion and exclusion criteria, 135 studies were included in the detailed analysis. Based on the model characteristics, the studies were classified into five subject groups: haematogenous osteomyelitis, post-traumatic osteomyelitis, bone-implant-related infection, peri-prosthetic joint infection, fracture-related infection. In addition, the characteristics of the mice used, such as inbred strain, age or gender, the characteristics of the pathogens used, the inoculation methods, the type of anaesthesia and analgesia used during surgery and the procedures for evaluating the pathogenicity of the infecting micro-organism were described. Overall, the mouse is an excellent first step in vivo model to study the pathogenesis, inflammation and healing process of osteomyelitis and to evaluate novel prophylaxis and treatment strategies.

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Inhibitory effect of roxithromycin on the local levels of bone-resorbing cytokines in an experimental model of murine osteomyelitis

Abstract: These results indicate that roxithromycin suppresses the local expression of IL-1beta and TNF-alpha, and may exhibit an anti-inflammatory effect in this osteomyelitis model.

Cited by 12 publications

References 6 publications

A systematic review of animal models for Staphylococcus aureus osteomyelitis

A systematic review of animal models for Staphylococcus aureus osteomyelitis

Apoptotic Bodies Convey Activity Capable of Initiating Osteoclastogenesis and Localized Bone Destruction

Current osteomyelitis mouse models, a systematic review

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