Inhibitory effect onStreptococcus mutansand mechanical properties of the chitosan containing composite resin

Abstract

ObjectivesThis study evaluated the antibacterial effect and mechanical properties of composite resins (LCR, MCR, HCR) incorporating chitosan with three different molecular weights (L, Low; M, Medium; H, High).Materials and MethodsStreptococcus (S). mutans 100 mL and each chitosan powder were inoculated in sterilized 10 mL Brain-Heart Infusion (BHI) solution, and was centrifuged for 12 hr. Absorbance of the supernatent was measured at OD660 to estimate the antibacterial activities of chitosan. After S. mutans was inoculated in the disc shaped chitosan-containing composite resins, the disc was cleansed with BHI and diluted with serial dilution method. S. mutans was spread on Mitis-salivarius bacitracin agar. After then, colony forming unit (CFU) was measured to verify the inhibitory effect on S. mutans biofilm. To ascertain the effect on the mechanical properties of composite resin, 3-point bending and Vickers hardness tests were done after 1 and 3 wk water storage, respectively. Using 2-way analysis of variance (ANOVA) and Scheffe test, statistical analysis was done with 95% significance level.ResultsAll chitosan powder showed inhibition effect against S. mutans. CFU number in chitosan-containing composite resins was smaller than that of control resin without chitosan. The chitosan containing composite resins did not show any significant difference in flexural strength and Vickers hardness in comparison with the control resin. However, the composite resin, MCR showed a slightly decreased flexural strength and the maximum load than those of control and the other composite resins HCR and LCR.ConclusionsLCR and HCR would be recommended as a feasible antibacterial restorative due to its antibacterial nature and mechanical properties.

Antibacterial capacity of cavity disinfectants against <i>Streptococcus mutans</i> and their effects on shear bond strength of a self-etch adhesive

“…Streptococcus mutans was chosen as the target microorganism because it is a well known major pathogen of caries 20) . These bacteria are small enough to move rapidly and penetrate easily into the dentinal tubules 21) , which may lead to pulp damage.…”

Antimicrobial properties of conventional restorative filling materials and advances in antimicrobial properties of composite resins and glass ionomer cements—A literature review

“…These included: [171] or a broth culture test [144], Methyl thiazolytetrazolium (MTT) assays [11,172], spectrophotometery [38,76,103,104,118], determination of colony forming units [2,16,17,86,96,104,129,160], determination of maximum or minimum inhibitory concentration (MIC) [4,12,13,22,71,169,172], minimum bactericidal concentration [172], direct contact test [39,64,65,77,78,97,109,120,122] and SEM [11,17,103,113,139]. Another method is through viability staining [94], Live/Dead BacLight ® bacterial viability stain has also been was used in some studies to provide data on the amounts of viable bacteria [4,96,104,172].…”

“…Other additives to composites and GICs found to be effective against cariogenic bacteria include cetylpyridium chloride, cetrimide [148,150,155,156], benzalkonium chloride [95,146], sodium fusidate [157], 2,2 -bipyridine [158], chitosan [159,160], triclosan [66,149,[161][162][163], propolis [164,165], anthocyanin [166], tetrapod-like zinc oxide whisker (T-ZnOw) [167] and epigallocatechin-2-gallate (EGCG) [11,168,169]. In contrast to most of the other additives, addition of T-ZnOw epigallocatechin-2-gallate resulted in improved mechanical properties [11,167].…”

Antibacterial properties of composite resins incorporating silver and zinc oxide nanoparticles onStreptococcus mutansandLactobacillus

“…It has been demonstrated that more microbial plaque is formed on the surface of composite resin restorations compared to amalgam and glass-ionomers 4. As a result of the absence of antibacterial activity in composite resins, attempts have been made to incorporate antibacterial agents such as chlorhexidine, methacryloyloxydodecylpyridinium bromide (MDPB) monomer, ursolic acid and chitosan into composite resins and bonding agents to provide therapeutic effects and antibacterial activity 1,5,6,7. One of the methods suggested is addition of certain antibacterial agents at nano levels to dental composite resins 8,9,10…”