Inhibitory effect on TNF-α-induced IL-8 secretion in HT-29 cell line by glyceroglycolipids from the leaves of Ficus microcarpa

Kiệm, Phan Văn; Minh, Châu Văn; Nhiệm, Nguyễn Xuân; Cường, Nguyễn Xuân; Tài, Bùi Hữu; Quang, Trần Hồng; Anh, Hoang Le Tuan; Yến, Phạm Hải; Ban, Ninh Khac; Kim, Seung Hyun; Xin, Mingjie; Yun, Ji; Lee, Young Mi; Kim, Young Ho

doi:10.1007/s12272-012-1210-8

Cited by 18 publications

(11 citation statements)

References 12 publications

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“… 2001 ), ( 2 S )-1- O -linolenoyl-2- O -linolenoyl-3- O -β- d -galactopyranosyl- sn -glycerol ( 16 ) (Kiem et al. 2012 ), ( 2 S )-1- O -linolenoyl-2- O -linoleoyl-3- O -β- d -galactopyranosyl- sn -glycerol ( 17 ) (Ibrahim et al. 2011 ), ( 2 S )-1- O -linoleoyl-2- O -linoleoyl-3- O -β- d -galactopyranosyl- sn -glycerol ( 18 ) (Janwitayanuchit et al.…”

Section: Resultsmentioning

confidence: 99%

Antibacterial activities of chemical constituents from the aerial parts of Hedyotis pilulifera

Nguyen

et al. 2017

Pharmaceutical Biology

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Context:Hedyotis pilulifera (Pit.) T.N. Ninh (Rubiaceae) has been used in Vietnamese ethnomedicine; the methanol extract exhibited antibacterial activity in our preliminary screening.Objectives: In this study, compounds from H. pilulifera were isolated and their antibacterial activity in vitro was evaluated.Materials and methods: The aerial parts of H. pilulifera (1.4 kg) were extracted with MeOH, suspended in water and ethyl acetate extract was chromatographed on a silica gel column. The structures of isolated compounds were elucidated by the combination analyses of spectroscopy including 1D-, 2D-NMR, HRMS and in comparison with the reported NMR data in the literature. All isolated compounds were evaluated for inhibitory effect using the microdilution method toward Staphylococcus aureus, Bacillus subtilis and Mycobacterium smegmatis, and MIC values were determined.Results: Twenty compounds were isolated, including five triterpenoids, two steroids, two aromatic compounds, three fatty acids, one quinone derivative, one lignan glycoside, one ceramide and five glycolipids. Among these, oleanolic acid showed significant antibacterial activity against M. smegmatis with the MIC value of 2.5 μg/mL. Remarkably, rotungenic acid showed strong activity against S. aureus, B. subtilis, M. smegmatis with MIC values of 2.5, 2.5 and 1.25 μg/mL, respectively. Rotundic acid exhibited significant antibacterial activity against B. subtilis with the MIC value of 5 μg/mL. To the best of our knowledge, the antibacterial activity of rotungenic acid, stigmast-4-ene-3,6-dione and (2S,3S,4R,2′R)-2-(2′-hydroxytetracosanoylamino) octadecane-1,3,4-triol was reported for the first time.Conclusions: Oleanolic acid, rotungenic acid, and rotundic acid were considered to be useful for developing new antimicrobial therapeutic agents for human.

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Section: Resultsmentioning

confidence: 99%

Antibacterial activities of chemical constituents from the aerial parts of Hedyotis pilulifera

Nguyen

et al. 2017

Pharmaceutical Biology

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“…Based on the aforementioned data, Compound 3 was identified as a monogalactosyl diacylglyceride, (2 S )-1- O -linolenoyl-2- O -linolenoyl-3- O - β- d- galactopyranosyl- sn -glycerol, and the compound was named panaxcerol C ( Fig. 1 ) [21] .…”

Section: Resultsmentioning

confidence: 99%

“…Based on these results, the chemical structure of Compound 4 was determined to be 2( S )-1- O -linoleoyl-2- O -linoleoyl-3- O - β- d- galactopyranosyl- sn -glycerol, named panaxcerol D ( Fig. 1 ) [21] .…”

Section: Resultsmentioning

confidence: 99%

Glycosyl glycerides from hydroponic Panax ginseng inhibited NO production in lipopolysaccharide-stimulated RAW264.7 cells

Cha

Park

Shrestha

et al. 2015

Journal of Ginseng Research

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BackgroundAlthough the aerial parts of hydroponic Panax ginseng are reported to contain higher contents of total ginsenosides than those of roots, the isolation and identification of active metabolites from the aerial parts of hydroponic P. ginseng have not been carried out so far.MethodsThe aerial parts of hydroponic P. ginseng were applied on repeated silica gel and octadecylsilane columns to yield four glycosyl glycerides (Compounds 1–4), which were identified based on nuclear magnetic resonance, infrared, fast atom bombardment mass spectrometry, and gas chromatography/mass spectrometry data. Compounds 1–4 were evaluated for inhibition activity on NO production in lipopolysaccharide (LPS)-stimulated RAW264.7 cells.Results and conclusionThe glycosyl glycerides were identified to be (2S)-1-O-7(Z),10(Z),13(Z)-hexadecatrienoyl-3-O-β-d-galactopyranosyl-sn-glycerol (1), (2S)-1-O-linolenoyl-3-O-β-d-galactopyranosyl-sn-glycerol (2), (2S)-1-O-linolenoyl-2-O-linolenoyl-3-O-β-d-galactopyranosyl-sn-glycerol (3), and 2(S)-1-O-linoleoyl-2-O-linoleoyl-3-O-β-d-galactopyranosyl-sn-glycerol (4). Compounds 1 and 2 showed moderate inhibition activity on NO production in LPS-stimulated RAW264.7 cells [half maximal inhibitory concentration (IC50): 63.8 ± 6.4μM and 59.4 ± 6.8μM, respectively] without cytotoxicity at concentrations < 100μM, whereas Compounds 3 and 4 showed good inhibition effect (IC50: 7.7 ± 0.6μM and 8.0 ± 0.9μM, respectively) without cytotoxicity at concentrations < 20μM. All isolated compounds showed reduced messenger RNA (mRNA) expression of interleukin-1β (IL-1β), IL-6, and tumor necrosis factor-α in LPS-induced macrophage cells with strong inhibition of mRNA activity observed for Compounds 3 and 4.

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“…Tumor necrosis factor (TNF)- α is a pro-inflammatory cytokine produced from monocytes and macrophages after the invasion of various pathogens that attack the host defense mechanisms. TNF- α plays a crucial role in host defense and in inflammatory response [ 4 ]. Specifically, IL-6 is an important molecule because it exhibits pro- and anti-inflammatory effects, and has been implicated in several inflammatory diseases [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

Isolation and Identification of Benzochroman and Acylglycerols from Massa Medicata Fermentata and Their Inhibitory Effects on LPS-Stimulated Cytokine Production in Bone Marrow-Derived Dendritic Cells

et al. 2018

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Massa Medicata Fermentata (MMF), known as Shenqu, is an important traditional Chinese medicine widely used to treat indigestion, vomiting, and diarrhea. In this study, a new benzochroman, 3(S)-3,4-dihydro-5,10-di-β-d-glucopyranoside-2,2-dimethyl-2H-naphtho(2,3-b)pyran-3-ol (1), and five known galactosyl acylglycerols (2–6) were isolated from a methanol extract from MMF. In addition, their chemical structures were determined by chemical and spectroscopic methods, which were compared with the previously reported data. Furthermore, the effects of isolated compounds on lipopolysaccharide (LPS)-stimulated bone marrow-derived dendritic cells were investigated. Compounds 1–3 exhibited significant inhibitory effects on the LPS-induced production of IL-6 and IL-12 p40, with IC50 values ranging from 1.6 to 10.2 μM. Compounds 2 and 3 also exhibited strong inhibitory effects on the LPS-stimulated production of TNF-α with IC50 values of 12.0 and 11.2 μM, respectively. The results might provide a scientific basis for the development of the active components in MMF, as well as for novel anti-inflammatory agents.

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Inhibitory effect on TNF-α-induced IL-8 secretion in HT-29 cell line by glyceroglycolipids from the leaves of Ficus microcarpa

Cited by 18 publications

References 12 publications

Antibacterial activities of chemical constituents from the aerial parts of Hedyotis pilulifera

Antibacterial activities of chemical constituents from the aerial parts of Hedyotis pilulifera

Glycosyl glycerides from hydroponic Panax ginseng inhibited NO production in lipopolysaccharide-stimulated RAW264.7 cells

Isolation and Identification of Benzochroman and Acylglycerols from Massa Medicata Fermentata and Their Inhibitory Effects on LPS-Stimulated Cytokine Production in Bone Marrow-Derived Dendritic Cells

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