Inhibitory effects of a manganese superoxide dismutase isolated from garlic (Allium sativumL.) on in vitro tumoral cell growth

Abstract

Reactive oxygen species are implicated in cancer development and antioxidants in general and superoxide dismutases and superoxide dismutase mimetic in particular, and they inhibit malignant transformation. We examinated the effects of an isolated manganese superoxide dismutase from a medicinal plant Allium sativum. The protein was prepared by a serial of chromatographic techniques: gel filtration and diethylaminoethyl ions exchanger. The enzyme has a specific activity equal to 55 U/mg. Two tumoral cell lines, porcine endothelial cells and mouse melanoma cells were exposed to garlic superoxide dismutase. The exogenous manganese superoxide dismutase is able to modify the intracellular level of reactive oxygen species by eliminating superoxide anion and producing hydrogen peroxide. The cell viability of the two lines was not significantly affected but the cell multiplication was arrested. This effect obtained in the presence of manganese superoxide dismutase correlates with the activation and modulation of phospho-extracellular signal-regulated kinases proteins, implicated in the control of several biological processes including cell proliferation.

Toxicity and repellency of garlic (Allium sativumL.) extract grown in Iran againstTribolium castaneum(Herbst) larvae and adults

“…Garlic, A. sativum L. (Amaryllidaceae), has been playing an important dietary and medicinal role for centuries. Even today, the medicinal use of garlic remains popular all over the world (Namazi 2008;Li et al 2009;Sfaxi et al 2009). Through careful research, detailed information on the chemical compositions of garlic extract is now very clear, it is mainly dominated by sulphides (main compound is methyl allyl trisulphide (19.8%)), followed by diallyl trisulphide (16.3%), 2-vinyl-[4H]-1,3-dithiin (13.9%), 3-vinyl-[4H]-1,2-dithiin (11.1%), dimethyl trisulphide (10.1%), diallyl disulphide (7.2%) and methyl allyl disulphide (4.7%) (Wu et al 2004;Kimbaris et al 2009).…”

Management ofSitotroga cerealellain stored cereal grains: a review

“…Garlic, Allium sativum, belongs to family Amaryllidaceae have dietary, medicinal (Namazi 2008;Sfaxi et al 2009) and insecticidal properties (Kimbaris et al 2009;Yang et al 2009). It is essential oil is composed of methyl allyl disulphide and diallyl trisulphide (Shrotriya et al 2010).…”

In Vitro Cancer Research on Ancient Herbal Remedies: A Changing Trend

“…A manganese superoxide dismutase has been isolated from this herb with inhibitory effects on the growth of porcine endothelial cells and B16 mouse melanoma cells, in vitro (Sfaxi et al, 2009). Ajoene is another active compound isolated from A. sativum that has been tested against MCF-7, KB, Bel 7402 hepatocellular, BGC 823 gastric, HCT colon, HL60 promyeloleukemic, and HeLa cervical cancer cells .…”