Inhibitory effects of Angelicae Gigantis Radix on osteoclast formation

Abstract

Angelicae Gigantis Radix (AGR) is one of the most widely used herbal medications. AGR is the dried root of Angelica gigas Nakai (Umbelliferae), which is known as Korean angelica. This study investigated the effects of AGR on osteoclast formation using primary bone marrow cells. TNF-alpha treatment increased tartrate-resistant acid phosphatase (Trap) positive cells and Trap activity in bone marrow cells. However, AGR significantly decreased both TNF-alpha-induced Trap positive cells and Trap activity. RT-PCR analyses revealed that AGR decreased mRNA levels of Trap and matrix metalloproteinase-9 in TNF-alpha-treated bone marrow cells. In addition, AGR decreased TNF-alpha-induced activation of NF-kappaB. These results suggest that AGR has an inhibitory effect on the formation of osteoclasts and its effect is partially related to the NF-kappaB pathway.

Inhibitory effect of Ssanghwa-tang on bone loss in ovariectomized rats

“…They contain stimulative ingredients on osteoblast differentiation increasing bone formation and inhibitory ingredients on osteoclast differentiation reducing bone loss, which contribute together to the antiosteoporotic effect of herbal medicine (Qin et al 2008). Extracts of medicinal herbs (Rehmannia glutinosa Libosch and Angelicae gigantis radix) of which SHT is composed stimulate osteoblast proliferation and activity while inhibiting osteoclast differentiation or bone loss in OVX rats (Oh et al 2003;Kil et al 2008). The anabolic effect of SHT on osteoblast differentiation (Lee and Lim 2003) and the inhibitory effect of SHT on osteoclast differentiation (Figure 1) have been studied.…”

Anti-inflammatory Effect of Angelicae Gigantis Radix Water Extract on LPS-stimulated Mouse Macrophages

“…참당귀의 함유성분으로는 coumarin계의 decursin, decursinol angelate 와 nodakentin, umbelliferon, β -sitosterol 등이 알려져 있다 17) . 약리작용으로는 최근에 파 골세포 형성 저해효과 18) , 베타아밀로이드에 의한 기억력 장애 에 대한 보호효과 19) , 뇌경색보호효과 이와 같이 AG가 LPS에 의해 유발된 마우스 대식세포의 다양한 사이토카인(cytokine) 생성증가를 유의하게 억제함은 AG가 대식세포의 염증매개인자과잉생성으로 인한 각종의 염 증성질환악화, 예를 들면 류마티스성관절염 악화 37) , 폐렴 및 급성 호흡기질환의 악화 38) , 자궁내막증식증(endometriosis) 유발촉진 39 …”

Decursin inhibits osteoclastogenesis by downregulating NFATc1 and blocking fusion of pre-osteoclasts

“…Angelica gigas NAKAI (AGN) and its substances have been reported to exhibit several biological activities including anti-tumor, anti-nociceptive, anti-bacterial, and anti-inflammatory activity [19][20][21][22][23]. Especially, AGN inhibited osteoclast formation and exhibited anti-osteoporotic activity in ovariectomized rats [24,25], and decursin (Fig. 1A) as the major active compound of AGN root, exhibited anti-inflammatory activity in macrophages [26,27].…”