Inhibitory effects of flavonoids on biofilm formation by Staphylococcus aureus that overexpresses efflux protein genes

Lopes, Laênia Angélica Andrade; Rodrigues, Jéssica Bezerra dos Santos; Magnani, Marciane; Souza, Evandro Leite de; Siqueira-Júnior, José P.

doi:10.1016/j.micpath.2017.03.033

Cited by 108 publications

(57 citation statements)

References 28 publications

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“…This study suggested that phloretin also acts as an inhibitor of biofilm formation as well as an anti-inflammatory agent in inflammatory diseases [37]. Besides, Phloretin exhibited anti-biofilm formation of S. aureus RN4220 and SA1199B at low concentration with inhibitory efficiency up to 70% [38], which might function through targeting efflux proteins.…”

Section: Phloretinmentioning

confidence: 77%

Developing natural products as potential anti-biofilm agents

et al. 2019

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Biofilm is a natural form of bacterial growth ubiquitously in environmental niches. The biofilm formation results in increased resistance to negative environmental influences including resistance to antibiotics and antimicrobial agents. Quorum sensing (QS) is cell-to-cell communication mechanism, which plays an important role in biofilm development and balances the environment when the bacteria density becomes high. Due to the prominent points of biofilms implicated in infectious disease and the spread of multi-drug resistance, it is urgent to discover new antibacterial agents that can regulate biofilm formation and development. Accumulated evidences demonstrated that natural products from plants had antimicrobial and chemo-preventive properties in modulation of biofilm formation in the last two decades. This review will summarize recent studies on the discovery of natural anti-biofilm agents from plants with clear-cut mechanisms or identified molecular addresses, as well as some herbs with unknown mechanisms or unidentified bioactive ingredients. We also focus on the progression of techniques on the extraction and identification of natural anti-biofilm substances. Besides, anti-biofilm therapeutics undergoing clinical trials are discussed. These newly discovered natural anti-biofilm agents are promising candidates which could provide novel strategies for biofilm-associated infections.

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Section: Phloretinmentioning

confidence: 77%

Developing natural products as potential anti-biofilm agents

et al. 2019

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“…In another study, myricetin ( 46 ) inhibited biofilm formation of S . aureus by MBIC 50 values of 1 μg/ml (Lopes, dos Santos Rodrigues, Magnani, de Souza, & de Siqueira‐Júnior, ).…”

Section: Antibacterial Activity Of Flavonoidsmentioning

confidence: 99%

Antibacterial activity of flavonoids and their structure–activity relationship: An update review

Farhadi

Khameneh

Iranshahi

et al. 2018

Phytotherapy Research

487

305

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Based on World Health Organization reports, resistance of bacteria to well-known antibiotics is a major global health challenge now and in the future. Different strategies have been proposed to tackle this problem including inhibition of multidrug resistance pumps and biofilm formation in bacteria and development of new antibiotics with novel mechanism of action. Flavonoids are a large class of natural compounds, have been extensively studied for their antibacterial activity, and more than 150 articles have been published on this topic since 2005. Over the past decade, some promising results were obtained with the antibacterial activity of flavonoids. In some cases, flavonoids (especially chalcones) showed up to sixfold stronger antibacterial activities than standard drugs in the market. Some synthetic derivatives of flavonoids also exhibited remarkable antibacterial activities with 20-to 80-fold more potent activity than the standard drug against multidrug-resistant Gram-negative and Gram-positive bacteria (including Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus). This review summarizes the ever changing information on antibacterial activity of flavonoids since 2005, with a special focus on the structure-activity relationship and mechanisms of actions of this broad class of natural compounds.

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“…All limonoids (25)(26)(27) showed a characteristic peak at m/z 189 derived from the common cleavage of ring C, followed by rearrangements, and the subsequent loss of H 2 O, which confirmed the presence of an epoxylactone system and an intact furan ring ( Figure 2, Table 2). In addition, the RDA cleavage of ring C provided a weak but diagnostically useful peak at m/z 295, suggesting a limonin skeleton ( Figure 2).…”

Section: Antibacterial Activitymentioning

confidence: 78%

“…In addition, the RDA cleavage of ring C provided a weak but diagnostically useful peak at m/z 295, suggesting a limonin skeleton ( Figure 2). The limonoids limonyl acetate (25) (27) was previously isolated from seeds of C. sinensis, 19 and it was found in all the root samples analyzed (Table 1).…”

Section: Antibacterial Activitymentioning

confidence: 99%

Rapid differentiation of graft Citrus sinensis with and without Xylella fastidiosa infection by mass spectrometry

Soares

Silva

Amaral

et al. 2020

Rapid Comm Mass Spectrometry

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Rationale Xylella fastidiosa causes citrus variegated chlorosis (CVC) in sweet orange trees. A diagnostic method for detecting CVC before the symptoms appear, which would inform citrus producers in advance about when the plant should be removed from the orchard, is essential for reducing pesticide application costs. Methods Chemometrics was applied to high‐performance liquid chromatography diode array detector (HPLC‐DAD) data to evaluate the similarities and differences between the chromatographic profiles. A liquid chromatography/atmospheric pressure chemical ionization mass spectrometry selected reaction monitoring (LC/APCI‐MS‐SRM) method was developed to identify the major compounds and to determine their amounts in all samples. Results We evaluated the effect of this bacterium on the variation in the chemical profile in citrus plants. The organs of C. sinensis grafted on C. limonia were analyzed. Chemometrics was applied to the obtained data, and two major groups were differentiated. Flavonoids were observed in one group (leaves) and coumarins in the second (roots), both at higher concentrations in the plants with CVC symptoms than in those without the symptoms and those in the negative control. The rootstocks also interfered in the metabolism of the scion. Conclusions The developed LC/APCI‐MS‐SRM method for detecting CVC before the symptoms appear is simple and accurate. It is inexpensive, and many samples can be screened per hour using 1 mg of leaves. Knowledge of the influence of the rootstock on the chemical profile of the graft is limited. This study demonstrates the effect of the rootstock in synthesizing flavonoids and increasing its content in all parts of the graft.

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Inhibitory effects of flavonoids on biofilm formation by Staphylococcus aureus that overexpresses efflux protein genes

Cited by 108 publications

References 28 publications

Developing natural products as potential anti-biofilm agents

Developing natural products as potential anti-biofilm agents

Antibacterial activity of flavonoids and their structure–activity relationship: An update review

Rapid differentiation of graft Citrus sinensis with and without Xylella fastidiosa infection by mass spectrometry

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