Inhibitory Effects ofDendrobiumAlkaloids on Memory Impairment Induced by Lipopolysaccharide in Rats

Abstract

Dendrobium alkaloids (DNLA), extracted from Dendrobium nobile Lindl. whose botanical name is Dendrobium moniliforme, Orchidaceae family, were studied for their effect on lipopolysaccharide (LPS)-induced memory impairment in rats. SD rats were pretreated with DNLA (40, 80, 160 mg/kg/d for 7 d), followed by LPS (50 µg) injection into the right lateral ventricle to produce memory impairment. DNLA treatment continued for another 13 days. The spatial behavior was tested by the Morris water maze; the level of tumor necrosis factor receptor 1 (TNFR1) mRNA was detected by real time RT-PCR, and the protein level of TNFR1, nuclear factor kappa-B (NF- κB) and phosphorylated p38 mitogen-activated protein kinases (p-p38 MAPK) by Western blotting. The results showed that DNLA significantly improved the neurobehavioral performance and prevented LPS-induced elevation in TNFR1 mRNA and protein levels. LPS-induced activation of p38 MAPK and NF- κB pathway was also suppressed. In conclusion, DNLA is effective in protecting against LPS-induced brain impairment, and this effect is due, at least in part, to prevent overexpression of TNFR1 via inhibition of p-p38 MAPK and the downstream NF- κB signal pathway.

Dendrobium alkaloids prevent Aβ25–35-induced neuronal and synaptic loss via promoting neurotrophic factors expression in mice

“…(DNLA) showed neuro-protective activity. For example, DNLA can prevent neuronal damages induced by LPS (Li et al, 2011; Zhang et al, 2011), and oxygen-glucose deprivation and reperfusion (Wang et al, 2010), decrease neuronal apoptosis, hyperphosphorylation of tau protein (Yang et al, 2014) and A β deposition in rat brain (Chen et al, 2008). The present study aimed to explore the effects of DNLA in protecting neurons from Aβ 25–35 -induced neurotoxicity in mice, and analyzed the mechanism from the aspects of promoting the secretion of neurotrophic factors.…”

Syringic Acid Extracted fromHerba dendrobiiPrevents Diabetic Cataract Pathogenesis by Inhibiting Aldose Reductase Activity

“…In ancient Chinese medical literature dating back over a thousand years, Dendrobium extracts were reported to improve vision [11]; however, there is still no firm understanding of the chemical components and mechanism of these extracts on vision. Recent research indicates that Dendrobium extracts may also have other bioactive properties, including the induction of apoptosis in human gastric cancer cells [12], the facilitation of neuroprotection against stress-induced apoptosis in PC12 cells [13], the inhibition of lipopolysaccharide- (LPS-) induced memory impairment in rats [14], and the LPS-induced nitric oxide production in macrophages [15]. It has also been suggested that Dendrobium extracts have strong antioxidant properties in vitro [16].…”

Floral morphology and reproductive biology of Dendrobium jiajiangense (Orchidaceae) in Mt. Fotang, southwestern China

“…Currently, most studies on Dendrobium mainly focus on its tissue culture (Pornpienpakdee et al, 2010;Rangsayatorn, 2009), planting methods (Bichsel et al, 2006) and medicinal compounds (Chang et al, 2010;Li et al, 2011). However, the relationships between species of Dendrobium and their pollinators have rarely been investigated.…”