Inhibitory effects ofIndigofera aspalathoideson 20-methylcholanthrene-induced chemical carcinogenesis in rats

Abstract

Background:The anticancer and antioxidant effects of the aqueous extract of Indigofera aspalathoides on 20-methylcholanthrene (20-MCA) induced fibrosarcoma were investigated in male albino rats.Materials and Methods:The rats were divided into four different groups, each group consisting of six animals. Group I animals were served as normal control, Group II animals were fibrosarcoma-bearing animals after the incubation period, Group III animals were fibrosarcoma-bearing animals, treated with aqueous extract of I. aspalathoides intraperitoneally at a dose of 250 mg/kg b.w. for 30 days and Group IV animals were administered with the aqueous extract of I. aspalathoides alone, at a dose of 250 mg/kg b.w. for 30 days, served as drug control animals. After the experimental period, all the rats were weighed and killed by cervical decapitation. The serum was separated from the blood for analysis. The weights of the liver and the kidneys were noted. The fibrosarcoma was proved by pathological examinations. The liver and kidney tissues were excised and then homogenized in an ice-cold buffer. These tissues were used for biochemical analysis.Results:The activities of antioxidant enzymes, e.g. catalase (CAT), glutathione peroxidase (GPx) and superoxide dismutase (SOD), in blood serum, liver, and kidney of control and experimental animals, respectively, have been reported.Conclusion: The present observations suggested that the aqueous extract of I. aspalathoides treatment enhanced the recovery from 20-MCA-induced fibrosarcoma due to its antioxidants and antineoplastic properties.

Fra-1 is a key driver of colon cancer metastasis and a Fra-1 classifier predicts disease-free survival

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Greater amygdala activity and dorsomedial prefrontal–amygdala coupling are associated with enhanced inflammatory responses to stress

“… 1 Two TNF-α samples (one at the BL1 time point, one at the 60 min time point) were below the detectable limit of the assay, and were thus set to half of the lower limit of detection (.25), a commonly-used approach for dealing with such values (LaFleur et al, 2011). Furthermore, we were unable to obtain a blood sample for one participant at the 60-min time point due to difficulty with the indwelling catheter.…”

Screening and computational analysis of colorectal associated non-synonymous polymorphism in CTNNB1 gene in Pakistani population

“…Auxiliary investigation of packing and stereochemistry was performed out and owing to several inconsistency; refinement was prepared to the preliminary model to clasp a superior model for further analysis. Energy minimization and structure refinements were made using GROMMACS available in Chimera 1.5.6 [36] and VEGA ZZ (http://www.ddl.unimi.it) .…”

“…Based on RMSD value of receptor ligand complex conformations cluetrs were generated. The best docked complex for CTNNB1 with GSK3 and TrcP1 were selected on the basis binding free energy value using ligplot [38], Discovery Studio visualizer ( http://accelrys.com/products/collaborative-science/biovia-discovery-studio ) and UCSF chimera [36] molecular interactions were mapped.…”