Inhibitory effects of <i>Leucaena leucocephala</i> on the metastasis and invasion of human oral cancer cells

Chung, Hsiao‐Hang; Chen, Mu‐Kuan; Chang, Yu‐Chao; Yang, Shun‐Fa; Lin, Chia-Chieh; Lin, Chiao‐Wen

doi:10.1002/tox.22399

Cited by 23 publications

(25 citation statements)

References 50 publications

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“…Natural substances, including traditional Chinese medications, greatly contribute to the blockage of tumour metastasis . Rheum palmatum L., a traditional Chinese medication, has been used for the treatment of many diseases such as constipation, jaundice, ulcers, and gastrointestinal hemorrhage .…”

Section: Introductionmentioning

confidence: 99%

Antimetastatic effects ofRheum palmatumL. extract on oral cancer cells

Chen

Hsieh

et al. 2017

Environmental Toxicology

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Rheum palmatum L., a traditional Chinese medication, has been used for the treatment of various disorders. However, the detailed impacts and underlying mechanisms of R. palmatum L. extracts (RLEs) on human oral cancer cell metastasis are still unclear. Here, we tested the hypothesis that an RLE has antimetastatic effects on SCC-9 and SAS human oral cancer cells. Gelatin zymography, Western blot, real-time polymerase chain reaction, and luciferase assay were used to explore the underlying mechanisms involved in the antimetastatic effects on oral cancer cells. Our results revealed that the RLE (up to 20 μg/mL, without cytotoxicity) attenuated SCC-9 and SAS cell motility, invasiveness, and migration by reducing matrix metalloproteinase (MMP)-2 enzyme activities. Western blot analysis of the MAPK signaling pathway indicated that the RLE significantly decreased phosphorylated ERK1/2 levels but not p38 and JNK levels. In conclusion, RLEs exhibit antimetastatic activity against oral cancer cells through the transcriptional repression of MMP-2 via the Erk1/2 signaling pathways. Thus, RLEs may be potentially useful as antimetastatic agents for oral cancer chemotherapy.

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Section: Introductionmentioning

confidence: 99%

Antimetastatic effects ofRheum palmatumL. extract on oral cancer cells

Chen

Hsieh

et al. 2017

Environmental Toxicology

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“…Studies have reported the MAPK signaling pathway as being involved in the regulation of MMP-2 expression in oral cancer [1,8]; thus, we examined the expression of three MAPK pathway-related proteins-ERK1/2, JNK1/2, and p38-using Western blot analysis. The results revealed that PSH reduced the phosphorylation level of ERK2 and p38 to 29% and 33%, respectively, in SCC-9 cells at 40 μM (Fig.…”

Section: Effect Of Psh On the Mapk Pathway In Scc-9 And Sas Cell Linesmentioning

confidence: 99%

“…Metastasis of cancer is a complex molecular mechanism that is often associated with cancer deaths [8]. During metastasis, degradation of the extracellular matrix (ECM) by extracellular proteinases contributes to the progression of tumor cell migration and invasion [9,10].…”

Section: Introductionmentioning

confidence: 99%

Pinostilbene Hydrate Suppresses Human Oral Cancer Cell Metastasis by Downregulation of Matrix Metalloproteinase-2 Through the Mitogen-Activated Protein Kinase Signaling Pathway

Hsieh

Chin

Lin

et al. 2018

Cell Physiol Biochem

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Background/Aims: Cancer is the most common cause of death worldwide with approximately one third of people being diagnosed with cancer in their lifetime. Pinostilbene hydrate (PSH) A methylated derivative of resveratrol Has been reported to possess antioxidative Cardioprotective and anticancer properties. However the antimetastatic effect of pinostilbene in oral squamous cell carcinoma (OSCC) remains unknown. Methods: In this study We investigated the effect of PSH on antimetastatic activity and the relevant signaling pathways underlying mechanisms of SCC-9 SAS and HSC-3 oral cancer cell lines by MTT assay Wound healing Transwell assay Zymography and western blot analysis. Results: Our findings indicated that PSH inhibits migration and invasion ability by reducing the protein activity and expression of matrix metalloproteinases-2 (MMP-2) in all three cell lines. Moreover • The phosphorylation of extracellular signal-regulated kinase (ERK) and p38 mitogen-activated protein kinases (p38) had significant inhibitory effects in the presence of PSH in the SCC9 and SAS cell lines. A combination of ERK1/2 and p38 inhibitors with PSH also reduced the migration and activity of MMP-2 in the SCC9 and SAS cell lines. Conclusion: This study demonstrated that PSH suppresses MMP-2 enzymatic activity by downregulating the p38/ERK1/2 pathway and that it might be a promising agent for preventing OSCC cell metastasis.

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“…Herbal compositions from natural compounds have been utilized in preclinical or clinical trials with chemopreventive or therapeutic drugs for cancer management . Although chemotherapy and targeted therapy are effective methods for cancer patients, these therapeutic processes have drawbacks of systemic toxicity and drug resistance .…”

Section: Discussionmentioning

confidence: 99%

“…Herbal compositions from natural compounds have been utilized in preclinical or clinical trials with chemopreventive or therapeutic drugs for cancer management. 20,[23][24][25][26][27] Although chemotherapy and targeted therapy are effective methods for cancer patients, these therapeutic processes have drawbacks of systemic toxicity and drug resistance. 28,29 To overcome these problems, studies on combined therapy have focused on identifying phytotherapeutic agents with known action mechanisms that can augment the therapeutic index of chemotherapeutic agents, such as Lentinan combined with oxaliplatin in hepatocellular carcinoma, 30 baicalein combined with docetaxel in thyroid cancer cells, 31 curcumin combined with platinum in ovarian cancer cells, 32 and Astragalus polysaccharides combined with cisplatin in nasopharyngeal carcinoma cells.…”

Section: Discussionmentioning

confidence: 99%

Licochalcone A induces apoptotic cell death via JNK/p38 activation in human nasopharyngeal carcinoma cells

Chuang

Tang

et al. 2019

Environmental Toxicology

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Licochalcone A is widely studied in different fields and possesses antiasthmatic, antibacterial, anti‐inflammatory, antioxidative, and anticancer properties. Its antimalignancy activity on renal, liver, lung, and oral cancer has been explored. However, limited studies have been conducted on the inhibitory effects of licochalcone A in human nasopharyngeal carcinoma cells. We determined cell viability using MTT assay. Cell cycle distribution and apoptotic cell death were measured via flow cytometry. Caspase activation and mitogen‐activated protein kinase‐related proteins in nasopharyngeal cancer cells in response to licochalcone A were identified by Western blot analysis. Results indicated that licochalcone A reduces cell viability and induces apoptosis, as evidenced by the upregulation of caspase‐8 and caspase‐9, caspase‐3 activation, and cleaved‐poly ADP‐ribose polymerase expression. Treatment with licochalcone A significantly increases ERK1/2, p38, and JNK1/2 activation. Co‐administration of a JNK inhibitor (JNK‐IN‐8) or p38 inhibitor (SB203580) abolishes the activation of caspase‐9, caspase‐8, and caspase‐3 protein expression during licochalcone A treatment. These findings indicate that licochalcone A exerts a cytostatic effect through apoptosis by targeting the JNK/p38 pathway in human nasopharyngeal carcinoma cells. Therefore, licochalcone A is a promising therapeutic agent for the treatment of human nasopharyngeal cancer cells.

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Inhibitory effects of Leucaena leucocephala on the metastasis and invasion of human oral cancer cells

Cited by 23 publications

References 50 publications

Antimetastatic effects ofRheum palmatumL. extract on oral cancer cells

Antimetastatic effects ofRheum palmatumL. extract on oral cancer cells

Pinostilbene Hydrate Suppresses Human Oral Cancer Cell Metastasis by Downregulation of Matrix Metalloproteinase-2 Through the Mitogen-Activated Protein Kinase Signaling Pathway

Licochalcone A induces apoptotic cell death via JNK/p38 activation in human nasopharyngeal carcinoma cells

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