Inhibitory effects of Indonesian temu kunci (<i>Boesenbergia rotunda</i>) rhizome extract on nitric oxide synthase production and on the kidneys of Wistar rats

Rosdianto, Aziiz Mardanarian; Puspitasari, Irma Melyani; Lesmana, Ronny; Sumiwi, Sri Adi; Megantara, Sandra; Jiranusornkul, Supat; Levita, Jutti

doi:10.3892/wasj.2022.173

Cited by 4 publications

(1 citation statement)

References 23 publications

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“…This compound blocked the activation of ERK1/2 and caspase 3 in cisplatin-treated mice (23). Furthermore, EEBR (doses of 1000 mg/kg and 2000 mg/kg) did not damage the kidney of Wistar rats as proven by histopathological examination (24).…”

Section: Discussionmentioning

confidence: 80%

The protective effect of Boesenbergia rotunda extract on cisplatin-exposed human embryonic kidney-293 cells by inhibiting the expression of kidney injury molecule-1, neutrophil gelatinase associated-lipocalin, NF-κB, and caspases

Sujana

Saptarini

Sumiwi

et al. 2022

J Herbmed Pharmacol

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Introduction: Acute kidney injury (AKI) is a major problem in platinum-based chemotherapy patients. Boesenbergia rotunda can induce the generation of osteoblast cells and significantly increase pancreatic antioxidant enzyme activities; therefore, this study aimed to investigate the cytotoxicity of cisplatin on human embryonic kidney-293 (HEK-293) cells and the protective impact of the ethanol extract of B. rotunda (EEBR) against such conditions. Methods: Cytotoxicity was assessed using the CCK-8/WST-8 reagent, while the protective activity was assayed on 1 µg/mL cisplatin-exposed HEK-293 cells by quantifying the expression of nephrotoxicity biomarkers, e.g., kidney injury molecule-1 (Kim-1) and neutrophil gelatinase associated-lipocalin (NGAL), nuclear factor-kappaB (NF-κB), apoptotic caspase-3, and caspase-7 genes, in cisplatin-exposed HEK-293 cells. Results: Cisplatin was confirmed as highly toxic against the HEK-293 cells (IC50 = 2.5145 μg/ mL), whereas quercetin was of moderate toxicity (IC50 = 185.6225 μg/mL). EEBR revealed an IC50 = 40.0655 μg/mL. Moreover, EEBR concentrations of 5, 10, and 20 µg/mL confirmed its remarkable protective activity against cisplatin-exposed HEK-293 cells (P=0.031, 0.014, 0.046, respectively) compared to the cisplatin-treated cell lines without treatment. The quantitative real-time polymerase chain reaction (PCR) revealed that a higher concentration of EEBR significantly suppressed the expression of Kim-1, while lower concentrations of EEBR significantly inhibited NGAL and NF-κB genes. Higher concentrations of EEBR reduced the expression of caspase-3. All concentrations of EEBR stimulated the expression of caspase-7. Conclusion: The significant protective activity observed in this study indicated that EEBR might be beneficial in protecting kidney cells against cisplatin.

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Section: Discussionmentioning

confidence: 80%

The protective effect of Boesenbergia rotunda extract on cisplatin-exposed human embryonic kidney-293 cells by inhibiting the expression of kidney injury molecule-1, neutrophil gelatinase associated-lipocalin, NF-κB, and caspases

Sujana

Saptarini

Sumiwi

et al. 2022

J Herbmed Pharmacol

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Nephroprotective Activity of Angelica keiskei (Miq). Koidz. on Cisplatin-Induced Rats: Reducing Serum Creatinine, Urea Nitrogen, KIM-1, and Suppressing NF-kappaB p65 and COX-2

Wahyuni,

Aulifa,

Rosdianto

et al. 2024

DDDT

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Background The sap of Angelica keiskei (Miq). Koidz. has been reported for its abundance of chalcone contents. Chalcones have been known for their effective nephroprotective activity toward cisplatin-induced renal cells and mice. Purpose To investigate the effect of A. keiskei sap extract (ASEE) on kidney function parameters (serum creatinine, urea nitrogen, and kidney injury molecule-1) and the expression of NF-kappaB p65 and COX-2 in cisplatin-induced Wistar rats. Methods In vivo nephroprotective activity of ASEE at 1000 and 1500 mg/kg BW/day doses for 10 days on cisplatin (5 mg/kg BW) induced nephrotoxicity was evaluated on Wistar rats. Quercetin 20 mg/kg BW/day was used as the control drug. Cisplatin inducement was given on day 7. The BW was measured every day. On day 11, the rats were euthanized, and their blood was taken intracardially for creatinine and urea nitrogen analysis. Histopathological analysis was carried out on the right kidney, and KIM-1 levels in the left kidney were measured. The Western blot technique evaluated the NF-kappaB p65 and COX-2 expression in the kidney. All data obtained were compared to the cisplatin group (negative control). The total flavonoids and chalcones in ASEE were also determined. Results Pretreatment with ASEE reduces the BW of Wistar rats, and significantly reduces creatinine and KIM-1 levels, but does not significantly reduce the levels of urea nitrogen, the expression of NF-kappaB p65, and COX-2 in the kidney of cisplatin-induced Wistar rats. The total flavonoid content in ASEE is 8.755 g QE/100 g extract and the total chalcone content is 5.532 g IBCE/100 g extract. Conclusion The sap of Angelica keiskei (Miq). Koidz. reveal the potential to protect the kidneys against cisplatin-induced toxicity. The nephroprotective activity may be attributed to the antioxidant and anti-inflammatory properties of the flavonoids and the chalcones contained in the sap of Angelica keiskei (Miq). Koidz.

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Boesenbergia rotunda (L.) Mansf.: A Review of Phytochemistry, Pharmacology, and Pharmacokinetics

Hop,

The Son

2023

COC

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Boesenbergia rotunda (the family Zingiberaceae) is a well-known medicinal plant from Southeast Asia and China. Its parts, especially the roots, were used as an alternative in cuisine, and flavoring. Many attempts tend to explore its phytochemistry and pharmacological values. The ultimate goal of this review is to critically offer the pharmacological values and mechanisms of action of B. rotunda crude extracts and their isolated compounds. Phytochemical investigation and pharmacokinetics were also discussed.On the basis of the most effective keyword “Boesenbergia rotunda”, the relevant data have been gathered from international electronic sources, including Sci-Finder, Google Scholar, PubMed, and reputation publishers. B. rotunda species was reported to contain two main chemical classes of phenolics and terpenoids. Its constituents have great biological values and spread out to drug development, including anticancer, anti-inflammatory, antimicrobacterial, antioxidative, ani-viral, anti-insect, antidiabetic, anti-allergic, anti-obesity, hepatoprotective, neuroprotective, rhenoprotective, gastroprotective, skin protective, vasorelaxant, and aphrodisiac activities. Various pharmacological actions were successfully proven by the underlying mechanisms. (–)-Panduratin A seems to be the most active compound with an oral bioavailability of 7-9%. B. rotunda plant extracts are safe in use with LD50 > 4.0 g/kg. Pharmacokinetic studies indicated that the metabolism of B. rotunda constituents may be related to the oxidation and glucuronidation of its phenolic compounds. Further phytochemical and pharmacological studies are expected, as well as clinical applications are urgently needed.

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Inhibitory effects of Indonesian temu kunci (Boesenbergia rotunda) rhizome extract on nitric oxide synthase production and on the kidneys of Wistar rats

Cited by 4 publications

References 23 publications

The protective effect of Boesenbergia rotunda extract on cisplatin-exposed human embryonic kidney-293 cells by inhibiting the expression of kidney injury molecule-1, neutrophil gelatinase associated-lipocalin, NF-κB, and caspases

The protective effect of Boesenbergia rotunda extract on cisplatin-exposed human embryonic kidney-293 cells by inhibiting the expression of kidney injury molecule-1, neutrophil gelatinase associated-lipocalin, NF-κB, and caspases

Nephroprotective Activity of Angelica keiskei (Miq). Koidz. on Cisplatin-Induced Rats: Reducing Serum Creatinine, Urea Nitrogen, KIM-1, and Suppressing NF-kappaB p65 and COX-2

Boesenbergia rotunda (L.) Mansf.: A Review of Phytochemistry, Pharmacology, and Pharmacokinetics

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