Inhibitory effects of methanol extracts of selected plants on the proliferation of two human melanoma cell lines

Fraihat, Alaa; Alatrash, Luma; Abbasi, Reem; Abu‐Irmaileh, Bashaer; Hamed, Saja; Mohammad, Mohammad; Abu-rish, Eman Y.; Bustanji, Yasser

doi:10.4314/tjpr.v17i6.15

Cited by 12 publications

(8 citation statements)

References 25 publications

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“…Tyrosinase inhibitors have been shown to have antiproliferative effects against melanoma cell lines. The inhibitory effects of the plant extracts discovered in the current study against the melanotic SK-Mel-5 cell line can be attributed, at least partially, to the tyrosinase inhibitory activity, which was consistent with a similar study by Fraihat et al [18].…”

Section: Discussionsupporting

confidence: 93%

Antiproliferative Effects of the Methanolic Petiole Extract of Eichhornia crassipes Against Sloan Kettering Melanoma 5 Cell Line: An In Vitro Study

Noufal¹,

Rajesh²,

Nair³

2022

Cureus

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BackgroundEichhornia crassipes (E. crassipes) have several secondary metabolites that have medicinal value. These include sterols, alkaloids, phenolics, flavonoids, tannins, and saponins. In the current study, the methanolic petiole extract of E. crassipes was examined to determine its potential antiproliferative activity against Sloan Kettering Melanoma 5 (SK-Mel-5) cell lines. Materials and methodsEichhornia crassipes were obtained from the water bodies of Ezhikkara, Ernakulam, Kerala. The Soxhlet technique was used to produce the extract. Leaves, petioles, and roots were dried and pulverized before being analyzed phytochemically in a number of solvents. The 3-(4,5-dimethylthiazol-2-yl)-2,5diphenyltetrazolium bromide (MTT) assay was used to measure the extent to which various dosages of the extract inhibited cell proliferation, and the methanolic extract of petioles was chosen since it contained more anticancer components. The half maximal inhibitory concentration (IC 50 ) was determined by utilizing a probit model and the slope-gradient method of the regression equation. The Statistical Package for Social Sciences (SPSS) version 21 (IBM SPSS Statistics, Armonk, NY) was used for the analysis. ResultsWe examined the effects of 12.5, 25, 50, 100, and 200 μg/ml of methanolic petiole extract. The data indicated that the methanol extract significantly reduced SK-Mel-5 cell viability. Cell growth inhibition increased with concentration but was shown to be relatively low at 100 g/ml, exhibiting 38.911% of inhibitory activity. The percentage of cell growth inhibition at 200 g/ml was 52.965%. The methanolic petiole extracts of E. crassipes were found to be cytotoxic with IC 50 values of 172.186 g/ml. Probit analysis was performed to obtain the regression equation. ConclusionThe in vitro study suggests that the methanol extract of the petiole of E. crassipes had modest antiproliferative action against SK-Mel-5 cells, a typical human melanocyte tumor cell line. The study findings shed light on the anticancer activity of E. crassipes, making it an appropriate source of drug-lead chemicals for the development of safer and cost-effective remedies for cutaneous ailments varying from rashes to awful melanoma.

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Section: Discussionsupporting

confidence: 93%

Antiproliferative Effects of the Methanolic Petiole Extract of Eichhornia crassipes Against Sloan Kettering Melanoma 5 Cell Line: An In Vitro Study

Noufal¹,

Rajesh²,

Nair³

2022

Cureus

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“…The IC 50 was 785 g/mL against MDA-MB-468 and 921 g/mL against MCF-7 for aerial parts extracts and 1560 g/mL against MCF-7 for root extracts. The methanolic extract of M. chamomilla has been tested by Fraihat et al [166] on two solid human melanocyte tumor cell lines, A375.S2 and WM1361A. In this study, results showed an inhibition only in the proliferation of the melanotic WM1361A cell line (IC 50 = 25.2 g/mL).…”

Section: Antidiabetic Activitymentioning

confidence: 56%

Chamomile (Matricaria chamomilla L.): A Review of Ethnomedicinal Use, Phytochemistry and Pharmacological Uses

Mihyaoui

Silva

Charfi

et al. 2022

Life

115

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Matricaria chamomilla L. is a famous medicinal plant distributed worldwide. It is widely used in traditional medicine to treat all kinds of diseases, including infections, neuropsychiatric, respiratory, gastrointestinal, and liver disorders. It is also used as a sedative, antispasmodic, antiseptic, and antiemetic. In this review, reports on M. chamomilla taxonomy, botanical and ecology description, ethnomedicinal uses, phytochemistry, biological and pharmacological properties, possible application in different industries, and encapsulation were critically gathered and summarized. Scientific search engines such as Web of Science, PubMed, Wiley Online, SpringerLink, ScienceDirect, Scopus, and Google Scholar were used to gather data on M. chamomilla. The phytochemistry composition of essential oils and extracts of M. chamomilla has been widely analyzed, showing that the plant contains over 120 constituents. Essential oils are generally composed of terpenoids, such as α-bisabolol and its oxides A and B, bisabolone oxide A, chamazulene, and β-farnesene, among other compounds. On the other hand, M. chamomilla extracts were dominated by phenolic compounds, including phenolic acids, flavonoids, and coumarins. In addition, M. chamomilla demonstrated several biological properties such as antioxidant, antibacterial, antifungal, anti-parasitic, insecticidal, anti-diabetic, anti-cancer, and anti-inflammatory effects. These activities allow the application of M. chamomilla in the medicinal and veterinary field, food preservation, phytosanitary control, and as a surfactant and anti-corrosive agent. Finally, the encapsulation of M. chamomilla essential oils or extracts allows the enhancement of its biological activities and improvement of its applications. According to the findings, the pharmacological activities of M. chamomilla confirm its traditional uses. Indeed, M. chamomilla essential oils and extracts showed interesting antioxidant, antibacterial, antifungal, anticancer, antidiabetic, antiparasitic, anti-inflammatory, anti-depressant, anti-pyretic, anti-allergic, and analgesic activities. Moreover, the most important application of M. chamomilla was in the medicinal field on animals and humans.

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“…The antiproliferative effect of the extract was tested on WM 1361A (high tyrosinase expression) using the MTT test. The results showed that M. chamomilla methanol extract inhibited the proliferation of melanotic WM1361A with an IC50 value of 25.2 g/ml (Fraihat et al, 2018).…”

Section: Passiflora Caerulea (Blue Passion Flower)mentioning

confidence: 98%

Phytochemical Profile and Anticancer Activity from Medicinal Plants Against Melanoma Skin Cancer: A Review

Fristiohady¹,

Asasutjarit²,

Purnama³

et al. 2022

Indonesian J. Sci. Technol

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Melanoma skin cancer is a malignant melanocyte tumor considered the most invasive and dangerous skin cancer, with an average five-year survival rate of less than 5% after metastasis. Thus, a new strategy for preventing and treating cancer from the natural product is required. Medicinal plants are the potential as an alternative against cancer. This review article aims to determine natural products from medicinal plants which have the potential as an anticancer in melanoma skin cancer in vitro and in vivo. 40 plants have been selected based on the selection criteria for anticancer compounds. In vitro studies showed that the plant can reduce cell viability through cell cycle inhibition and apoptosis induction and inhibit angiogenesis, invasion, and metastasis in human melanoma skin cancer. Therefore, further research is required to explore more plants, especially medicinal plants, their active compounds, and the mechanism of anticancer action to be used as standard herbal medicines.

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Inhibitory effects of methanol extracts of selected plants on the proliferation of two human melanoma cell lines

Cited by 12 publications

References 25 publications

Antiproliferative Effects of the Methanolic Petiole Extract of Eichhornia crassipes Against Sloan Kettering Melanoma 5 Cell Line: An In Vitro Study

Antiproliferative Effects of the Methanolic Petiole Extract of Eichhornia crassipes Against Sloan Kettering Melanoma 5 Cell Line: An In Vitro Study

Chamomile (Matricaria chamomilla L.): A Review of Ethnomedicinal Use, Phytochemistry and Pharmacological Uses

Phytochemical Profile and Anticancer Activity from Medicinal Plants Against Melanoma Skin Cancer: A Review

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