Inhibitory effects of polyphenol-enriched extract from Ziyang tea against human breast cancer MCF-7 cells through reactive oxygen species-dependent mitochondria molecular mechanism

Li, Wenfeng; He, Ningning; Tian, Lingmin; Shi, Xiaolong; Yang, Xingbin

doi:10.1016/j.jfda.2016.01.005

Cited by 30 publications

(29 citation statements)

References 56 publications

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“…In 2016, Li et al demonstrated that green tea preparation influenced internal oxidative stress of MCF-7 cells with an approximately 11-fold increase in ROS accumulation at 50 mg/mL dosage of preparation. Payen et al showed that in SiHa-F3 cells EGCG at concentrations of 100 nM, 1 μM, 10 μM and 100 μM revealed a growing antioxidant effect which was correlated with the EGCG concentration [26].…”

Section: Resultsmentioning

confidence: 99%

The influence of Viburnum opulus polyphenolic compounds on metabolic activity and migration of HeLa and MCF cells

Zakłos‐Szyda

Pawlik

2019

Acta Innovations

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In recent years, research of antitumor activity of natural compounds isolated from plant material has increased. Polyphenols have gained significant attention due to their proapoptotic abilities and their involvement in migration and inhibition of metastasis processes. The anticancer effects of polyphenolic extracts of Viburnum opulus fruit against human breast (MCF-7) and cervical (HeLa) cancer cell lines have been confirmed in this study. It was demonstrated that the tested preparations (methanol – M and acetone – A from pomace, juice – J and juice after extraction to the solid phase SPE – PF) show cytotoxic activity and regulate the migration process of cancer cells. The degree of inhibition of cell migration was measured at two times - 24 h and 48 h after addition of the tested preparations. The highest toxicity towards both cell lines was demonstrated by the polyphenol fraction obtained after juice purification SPE (IC50 values at concentration of 63,541 and 19,380 μg/mL for HeLa and MCF cell lines, respectively). At the same time, the same preparation inhibited cell migration the most (nearly 70% compared to controls at both times at the concertation of 15 and 30 μg/mL). All preparations showed the antioxidant ability, but the Viburnum opulus juice (200 and 350 g/mL) and the preparation after its purification (15 and 30 μg/mL) have larger ability to inhibit the intracellular oxidative stress (30-40%) than preparation obtained from pomace (nearly by 20% at concentration of 20 and 50 μg/mL of M and A). Despite the antioxidative capacity of the preparations, they simultaneously decreased cellular mitochondrial potential. The results obtained indicate the high potential of components of Viburnum opulus polyphenolic compounds can be used in the production of innovative dietary supplements or pharmacological preparations for people with an increased risk or inclination towards developing breast or cervical cancer.

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Section: Resultsmentioning

confidence: 99%

The influence of Viburnum opulus polyphenolic compounds on metabolic activity and migration of HeLa and MCF cells

Zakłos‐Szyda

Pawlik

2019

Acta Innovations

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“…Previous studies suggest that EGCG, EGC, ECG, EC, and C are the major catechins in tea, with their antioxidant activities demonstrated in the following order: EGCG > ECG > EGC > EC > C (Li et al, ; Spadiene et al, ). It is widely recognized that polyphenols of different molecular structures present different antioxidant activities.…”

Section: Resultsmentioning

confidence: 98%

“…Although the dark tea infusion did contain EGCG, its concentration was very low, resulting in quantitative analysis failure in some cases (Tables and ). It is well known that catechin compounds (EGCG, ECG, EGC, EC) are the most abundant flavonoids in tea (Li, He, Tian, Shi, & Yang, ); however, they are degraded by microbial enzymes and autoxidized during the fermentation process of black and dark teas (Jiang et al, ). In particular, EGCG can be metabolized by microorganisms to gallic acid and other compounds (Jiang et al, ; Takagaki, Kato, & Nanjo, ).…”

Section: Resultsmentioning

confidence: 99%

Citric acid‐enhanced dissolution of polyphenols during soaking of different teas

Zhang

Tan

et al. 2019

J Food Biochem

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This study examined the dissolution kinetics and antioxidant activity of tea polyphenols during the soaking of white, yellow, green, oolong, black, and dark teas. All these teas were, respectively, soaked with either freshly boiled distilled water (DW) or 10 mmol/L citric acid–water solution. The residue obtained from one extraction was used for the next extraction and this process was performed consecutively 10 times, soaking for 30 s each time. UHPLC‐QqQ‐MS measurement identified epigallocatechin gallate as the major polyphenol in white, yellow, green, oolong, and black tea infusions. As soaking times increased, the polyphenol concentrations rose initially and then dropped. Antioxidant activity was noted to decrease as soaking times increased in all tea infusions except for the DW‐soaked oolong and dark teas. These findings suggested that citric acid could increase the polyphenol content in tea infusions. Specifically, the cumulative contents of epigallocatechin gallate was noted to increase 2.1–5.1 times. Practical applications After water, tea is one of the most consumed beverages in the world. Drinking tea has been linked to the reduced risk of various diseases, including cancer, cardiovascular system disease, obesity, and neurodegenerative diseases, largely due to its rich polyphenol content. Moreover, the addition of citrus to a tea infusion provides an interesting and feasible method for increasing the dissolution of tea polyphenols, a finding that offers extensive potential for applications in the development of compound tea drinks.

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“…A diverse array of compounds with numerous biological properties account for these potential benefits. Phytochemicals, including flavonoids found in tea, have proven effective against chronic diseases, including colon cancer [33].…”

Section: Discussionmentioning

confidence: 99%

Chemopreventive Potential of Select Herbal Teas and Spices on Azoxymethane-Induced Aberrant Crypt Foci in Fisher 344 Male Rats

Willis¹,

Sunkara²,

Willis³

et al. 2017

FNS

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Colon cancer is the third leading cause of death in the US. Selected herbal teas and spices may reduce incidence of chronic diseases, including cancer. The objective of this study was to identify the effect of strawberry leaf, raspberry leaf, hibiscus teas and cinnamon on azoxymethane (AOM)-induced aberrant crypt foci (ACF) in Fisher 344 male rats. After acclimatization period (1 wk), 49 male weanling rats were divided into 16 groups. Control (CON) group fed AIN-93G diet; 15 treatment groups were administered control diet + strawberry leaf tea (STW), raspberry leaf tea (RAS), hibiscus tea (HIB), cinnamon (CIN), strawberry leaf tea + cinnamon (STW + CIN), raspberry leaf tea + cinnamon, (RAS + CIN), hibiscus tea + cinnamon (HIB + CIN), and strawberry leaf tea + raspberry leaf tea + hibiscus tea + cinnamon in combination (COM) at 2 levels each (teas added at 1% and 2%; CIN added at 2.5% and 5%). Rats received 24 mg/kg body weight AOM in saline s/c at 7 and 8 weeks of age. Animals received experimental diets until sacrificed by CO 2 asphyxiation (17 weeks of age). ACF were enumerated in colons. Hepatic antioxidant enzymes were determined; superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), and glutathione (GH). Treatment groups had reduction in ACF compared to CON (154). Lowest ACF observed in HIB 2% + CIN 5% (13.16) with 91.45% reduction compared to CON. ACF observed in treatment groups administered teas and cinnamon combinations were lower than those administered teas singly. SOD and CAT activities in rats administered treatment diets were higher than CON (13.63 U/mL, 0.95 umol·min). Rats administered COM (20.65 U/mL) had highest SOD activity. CAT activity was 51.27% higher in rats administered HIB 2% (1.96 umol·min −l ·ml −1 ). GPX activity ranged from 7.26 (STW 1% + CIN 2.5%) to 9.59 (STW 2%) umol·min −l ·ml −1. Results suggest that herbal teas and spices may reduce the risk of colon cancer and improve antioxidant status; regular consumption How to cite this paper:

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Inhibitory effects of polyphenol-enriched extract from Ziyang tea against human breast cancer MCF-7 cells through reactive oxygen species-dependent mitochondria molecular mechanism

Cited by 30 publications

References 56 publications

The influence of Viburnum opulus polyphenolic compounds on metabolic activity and migration of HeLa and MCF cells

The influence of Viburnum opulus polyphenolic compounds on metabolic activity and migration of HeLa and MCF cells

Citric acid‐enhanced dissolution of polyphenols during soaking of different teas

Chemopreventive Potential of Select Herbal Teas and Spices on Azoxymethane-Induced Aberrant Crypt Foci in Fisher 344 Male Rats

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