Inhibitory Effects ofBifidobacterium longumon EnterohemorrhagicEscherichia coliO157: H7

Abstract

The effects of Bifidobacterium longum BB536 (BB536) on Escherichia coli O157:H7 IPH9 (IPH9) were first examined using germ-free mice mono-associated with BB536 (BB536-MA). Thirty-two days after oral challenge with a lethal dose of IPH9, all the non-associated germ-free mice (GF) died, but all the BB536-MA mice survived and the number of IPH9 in the feces was lower than that in GF mice. Second, co-culture experiments with BB536 and IPH9 were performed. The number of IPH9 in the co-culture was less than 1% of that in mono-culture after 24 hr, and the verotoxin concentration also decreased. Culture of IPH9 under various conditions showed that lactate, acetate and the supernatant of BB536 culture had anti-O157 activities even when the pH was neutral, and the supernatant of BB536 culture had stronger inhibitory effects than lactate or acetate against the production of verotoxin. This efficient inhibition of verotoxin may suggest the presence of verotoxin-inhibitory factors in BB536 metabolites in addition to lactate and acetate.

Prevention of Escherichia coli O157:H7 infection in gnotobiotic mice associated with Bifidobacterium strains

“…In addition, lesions in the kidneys, but not in the intestines, were observed in GF Swiss Webster mice infected with EHEC (Eaton et al 2008). Previously, Aiba et al (2002) and Namba et al (2003) investigated E. coli O157:H7, using GF BALB/c mice. Namba et al (2003) reported that mild lesions were induced in the kidneys.…”

“…Previously, Aiba et al (2002) and Namba et al (2003) investigated E. coli O157:H7, using GF BALB/c mice. Namba et al (2003) reported that mild lesions were induced in the kidneys. They did not describe lesions in the intestines.…”

“…Various animal models including infant rabbits (Pai et al 1986;Ritchie et al 2003), gnotobiotic (GB) piglets (Francis et al 1986(Francis et al , 1989Tzipori et al 1988;Echtenkamp et al 2008), cows (Brown et al 1997;Dean-Nystrom et al 1997), mice (Fujii et al 1994;Lindgren et al 1993;Wadolkowski et al 1990a, b;Aiba et al 2002;Namba et al 2003;Takahashi et al 2004;Calderon Toledo et al 2008), monkeys (Kang et al 2001), and chickens (Berry et al 1995;Schoeni and Doyle 1994;La Ragione et al 2005) have been developed as EHEC infection models. Among these models, mouse models are commonly used.…”

Evaluation of the probiotic potential of Lactobacillus paracasei KW3110 based on in vitro tests and oral administration tests in healthy adults

“…There are also some reports that oral intake of probiotics can improve bowel movement disturbance such as diarrhea (Gismondo et al, 1999) and coprostasis (Fukushima et al, 2004;Yaeshima et al, 1997). In addition, probiotics have been reported to have a prophylactic effect against infectious diseases (Namba et al, 2003) and an ameliorating effect on inflammatory bowel diseases (Fukushima et al, 2004;Gionchetti et al, 2000).…”

Bifidobacterium longum-fermented Broccoli Supernatant Inhibited the Growth of Candida albicans and Some Pathogenic Bacteria in vitro

“…Lactic acid bacteria including bifidobacteria produce acetic acid and/or lactic acid, which tend to lower the intestinal pH and thus inhibit the proliferation of pathogenic microorganisms (Collins and Gibson, 1999;Gomes and Malcata, 1999). Bifidobacterium longum is found mainly in human feces (Mitsuoka, 1982), as well as Lactobacillus acidophilus, and reported to have protective effects against harmful intestinal bacteria such as Escherichia coli (Ibrahim and Bezkorovainy, 1993;Kojima et al, 1995;Namba et al, 2003). On the other hand, some lactic acid bacteria have been used in the preservation of foods because of their inhibiting activity against food poisoning and spoilage bacteria such as Salmonella Typhimurium and Staphylococcus aureus (Raccach et al, 1979).…”