Inhibitory function of P-selectin-mediated leukocyte adhesion by the polysaccharides fromSanguisorba officinalis

Abstract

Context: P-selectin is a promising target for inflammatory-related diseases. Polysaccharides are the active ingredients of Sanguisorba officinalis L. (Rosaceae) responsible for its antiinflammatory activities; however, the molecular mechanism is not clear yet.Objective: This study evaluates the effects of polysaccharides (SOPs) from Sanguisorba officinalis on their antagonistic function against P-selectin-mediated leukocyte adhesion. Materials and methods:The antagonistic function of SOPs was investigated by flow cytometry and static adhesion assay at the concentrations of 25 and 100 mg/ml. The dynamic interaction between HL-60 cells and CHO-P cell monolayer treated with SOPs (25 and 100 mg/ml) was analyzed in a parallel plate flow chamber, and quantitatively calculated by ImageJ software (NIH, Bethesda, MD). In vitro protein binding assay was carried out to evaluate the blocking effects of SOPs (25 and 100 mg/ml) on the interaction between P-selectin and PSGL-1. Results: SOPs-treatment (100 mg/ml) significantly reduced the percentage of HL-60 cells binding to P-selectin (p50.01) determined by flow cytometry. In addition, SOPs (25 and 100 mg/ml) markedly blocked the adhesion between HL-60 cells and CHO-P cells under static condition, and the inhibitory rates reached 39.9% and 71.2%, respectively. Compared with the positive control group, SOPs-treatment (25 and 100 mg/ml) significantly reduced the percentage of HL-60 cells rolling on CHO-P cell monolayers by 43.5% and 75.2%, respectively. Protein binding assay showed the interaction between P-selectin and PSGL-1 was significantly blocked by SOPs. Discussion and conclusion: SOPs possess a significant antagonistic function against P-selectin-mediated leukocyte adhesion, and SOPs could be considered as a promising candidate for amelioration of inflammation-related diseases.

Naturally occurring cell adhesion inhibitors

“…Effects of crude plant extract, snake venom and other naturally occurring fatty acid derivatives on cell adhesion molecules are also reported. As they are not isolated as pure components, only references are shown [ 36 – 45 ].…”

A Comprehensive Review of Genus Sanguisorba: Traditional Uses, Chemical Constituents and Medical Applications

“…In vitro anti-inflammatory tests conducted on zebrafish indicate that the gallic acid group could be the key bioactive group in the terpene glycosides present in S. officinalis , and might function as regulators of the distribution of zebrafish macrophages ( Guo et al, 2019 ). Polysaccharides from S. officinalis, when used at concentrations of 25 mg/ml and 100 mg/ml, exhibit evident antagonistic effects on P-selectin-mediated leukocyte adhesion, which is a promising target for the treatment of inflammation-related diseases ( Tong et al, 2015 ). Two acidic polysaccharides purified from S. officinalis ( Zhao et al, 2019 ), and ellagic acid ( Seo et al, 2016 ), which is considered a marker component of S. officinalis , have demonstrated significant inhibition of the production of pro-inflammatory cytokines TNF-α and IL-6 in RAW264.7 cells stimulated by LPS in vitro .…”

Antiangiogenic Effects of Ziyuglycoside II, a Major Active Compound of Sanguisorba officinalis L.

“…It possesses a variety of pharmacological properties including antioxidant , antitumor (Choi et al, 2012;Shin et al, 2012), antiangiogenic , and immunomodulatory effects (Cai et al, 2012;Liang et al, 2013). In addition, a variety of chemical constituents have been isolated from S. officinalis, and their biological properties have been evaluated (Tong et al, 2015). Among these constituents, ziyuglycosides, a group of primary terpenoid constituents, have a wide range of biological actions, including hemostasis (Sun et al, 2012) and reducing skin aging (Kim et al, 2008).…”