Inhibitory phlorotannins from the edible brown algaecklonia stolonifera on total reactive oxygen species (ROS) generation

Abstract

Reactive oxygen species (ROS) play an important role in the pathogenesis of many human degenerative diseases such as cancer, aging, arteriosclerosis, and rheumatism. Much attention has been focused on the development of safe and effective antioxidants. To discover sources of antioxidative activity in marine algae, extracts from 17 kinds of seaweed were screened for their inhibitory effect on total ROS generation in kidney homogenate using 2',7'-dichlorofluorescein diacetate (DCFH-DA). ROS inhibition was seen in three species: Ulva pertusa, Symphyocladia latiuscula, and Ecklonia stolonifera. At a final concentration of 25 microg/mL, U. pertusa inhibited 85.65+/-20.28% of total ROS generation, S. latiscula caused 50.63+/-0.09% inhibitory, and the Ecklonia species was 44.30+/-7.33% inhibition. E. stolonifera Okamura (Laminariaceae), which belongs to the brown algae, has been further investigated because it is commonly used as a foodstuff in Korea. Five compounds, phloroglucinol (1), eckstolonol (2), eckol (3), phlorofucofuroeckol A (4), and dieckol (5), isolated from the ethyl acetate soluble fraction of the methanolic extract of E. stolonifera inhibited total ROS generation.

Pattern of dietary fiber intake among the Japanese general population

“…As for the relationship of seaweed consumption to carcinogenesis, several reports have shown preventive effects of seaweeds against colorectal carcinogenesis in epidemiological studies (Tokudome et al, 1991;Hoshiyama et al, 1993), or in experimental studies in rats (Yamamoto and Maruyama, 1985;Teas et al, 1998); proposed mechanisms for such effects include inhibition of reactive oxygen species (Maruyama et al, 2003;Suzuki et al, 2004), or the modulation of natural killer cell activity (Teas, 1983). Furthermore, there are several reports regarding the preventive effect of seaweed consumption against breast cancer (Teas, 1983;Kang et al, 2004). However, it is important to recognize that to increase seaweed intake beyond current levels in Japan does not necessarily mean enhanced health benefits; indeed some researchers have shown disadvantages, for example, stimulatory effects upon cancers (Ashi et al, 1978;Watanabe et al, 1978;Reddy et al, 1985;Funahashi et al, 2001).…”

Cosmeceuticals Derived from Bioactive Substances Found in Marine Algae

“…Novel marine terpenoids show great promise as a source for new antioxidant agents in cosmetic preparations [8,9], due to their good penetration-enhancing abilities, low systemic toxicity and low irritation. Fucosterol (Figure 1) is a steroidal terpenoid extracted from Phaeophyta marine algae (Ecklonia stolonifera, Pelvetia siliquosa, Sargassum carpophyllum) [10][11][12].…”

Algae as potential repository of anti cancerous natural compounds

“…Also it may over express the level of reactive oxygen species [44]. Mono galactosyl diacylglycerols ( Figure. [47]. Ecklonia cava has an important compound name as fucoidan ( Figure. 3f) which is a mixture of sulfated Rhamnogalactofucan and galactofucan.…”