Inhibitory Properties of Ficus deltoidea on α-Glucosidase Activity

Adam, Zainah; Khamis, Shafii; Ismail, Amin; Hamid, Muhajir

doi:10.3923/rjmp.2010.61.75

Cited by 23 publications

(15 citation statements)

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“…Furthermore, increased concentration of substrate enhance this type of inhibition, This could be a reason of the increased percentage inhibition observed for α-glucosidase activity as the concentration of extract increase, delaying glucose production. This study is in agreement with previous reports which have shown that plant products exhibit strong inhibition against -glucosidase [18][19][20].…”

Section: Resultssupporting

confidence: 94%

Inhibitory Properties of Combined Ethanol Seed Extract of P. guineense and T. conophorum on -Glucosidase Enzyme

Dokubo¹,

Em²,

Jao³

2019

aabps

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The study was aimed to evaluate the α-glucosidase inhibitory properties of combined ethanol seed extract of Piper guineense and Tetracarpidium conophorum (CEPGTC). In vitro inhibitory assay was conducted in accordance with standard methods. p-nitrophenyl-D-glucopyranoside (pNPG) was used as a substrate. Fixed amount of the enzyme was incubated with increasing concentrations of CEPGTC seed extract (20-100 μg/ml) at 37°C for 15 min. The continuous production of p-nitrophenol was determined by measuring the absorbances at 410 nm. Acarbose at varying concentration (20-100g/ml) was used as the positive control. The median Inhibitory Concentration (IC50) values were graphically determined as the half-maximal inhibitory from plots of percent inhibition against log inhibitor concentration and by non-linear regression analysis. The enzyme kinetics (pattern of Inhibition) was determined using the Michaelis Menten's equation and Lineweaver-Burk plot. The result showed that there was inhibition in all concentrations evaluated. The IC 50 values estimated were 2.9 μg/ml and 2.5 μg/ml for CEPGTC and acarbose respectively. However, The IC 50 value for acarbose was significantly (p<0.05) different from CEPGTC which is an indication of acarbose as effective-glucosidase inhibitor thus, used as positive control. An enzyme kinetics studies from the Lineweaver-Burke plot showed that CEPGTC seed extract demonstrated an uncompetitive inhibition on α-glucosidase while acarbose exhibited non competive (mixed inhibition). The inhibitory response and mechanism of inhibition observed in this study showed that combined ethanol seed extract of Piper guineense and Tetracarpidium conophorum has the potential to reduce glucose turnover and improve Hyperglycemia.

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Section: Resultssupporting

confidence: 94%

Inhibitory Properties of Combined Ethanol Seed Extract of P. guineense and T. conophorum on -Glucosidase Enzyme

Dokubo¹,

Em²,

Jao³

2019

aabps

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“…Commercially, the dried leaves are packed in 1-2 g per sachet and recommended to infuse in 1 L of hot water. Earlier work has shown the crude extracts exhibited blood glucose lowering effect and a-glucosidase inhibiton (Adam et al, 2010;Ilyanie et al, 2011). Other species from the ficus genus, namely, F. religiosa Linn, F. racemosa Linn, F. bengalensis Linn, F. carica Linn, and F. exasperata Vahl.…”

Section: Introductionmentioning

confidence: 96%

Vitexin and isovitexin from the Leaves of Ficus deltoidea with in-vivo α-glucosidase inhibition

Choo

Sulong

Man

et al. 2012

Journal of Ethnopharmacology

204

117

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“…The leaves of F . deltoidea have been shown to exhibit blood glucose-lowering effects [19,20,22,24-26], antinociceptive [27], ulcer healing [28], antioxidant [19,21,29,30], anti-inflammatory [31] and antimelanogenic [32] properties. However, to date, there is no available report on the pharmacological activities of the fruits of F .…”

Section: Introductionmentioning

confidence: 99%

Antidiabetic and antioxidant properties of Ficus deltoidea fruit extracts and fractions

Misbah

Aziz

Aminudin

2013

BMC Complement Altern Med

103

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BackgroundDiabetes is a serious metabolic disorder affecting the metabolism of carbohydrate, protein and fat. A number of studies have shown that diabetes mellitus is associated with oxidative stress, leading to an increased production of reactive oxygen species. Ficus deltoidea is traditionally used in Malaysia for regulating blood sugar, blood pressure and cholesterol levels. The use of F. deltoidea as an alternative medicinal herb is increasingly gaining popularity with the sale of F. deltoidea tea bags and capsules in the local market. The present study was undertaken to investigate the antidiabetic and antioxidant activities of the fruits from different varieties of F. deltoidea, employing in vitro methods.MethodTwo fruit varieties of F. deltoidea (var. angustifolia (SF) and var. kunstleri (BF)) were extracted separately using double-distilled water. The resulting aqueous extracts were partitioned using ethyl acetate to obtain the ethyl acetate and water fractions. The crude aqueous extracts and the corresponding fractions were evaluated for their phenolic, flavonoid, sugar and protein contents. Protein profiling of the extracts and fractions were also carried out by means of SDS-PAGE and SELDI-TOF MS. Antidiabetic activities were assessed based on the ability of the samples to inhibit yeast and mammalian α-glucosidase as well as α-amylase. Antioxidant capacities were examined by measuring the ability of the samples to reduce ferric ions and to scavenge DPPH, superoxide anion, ABTS and nitric oxide radicals.ResultsThe crude extracts and fractions of SF and BF inhibited both yeast and rat intestinal α-glucosidases in a dose-dependent manner, but did not inhibit porcine pancreatic α-amylase. The water fraction of BF showed the highest percentage of α-glucosidase inhibition while having the highest amount of protein (73.33 ± 4.99 μg/mg fraction). All the extracts and fractions exhibited antioxidant activities, with SF crude extract showing the highest antioxidant activity and phenolic content (121.62 ± 4.86 mg/g extract). Fractionation of the crude extracts resulted in loss of antioxidant activities. There was no positive correlation between phenolic and flavonoid content with α-glucosidase inhibitory activities. However, phenolic content correlated well with antioxidant activities of the crude extracts but not with the fractions.ConclusionsThe antioxidant activities of the fruits of F. deltoidea might be asserted by the phenolic content but other polar plant components were possibly involved in the antidiabetic properties. The study of these compounds having both antihyperglycemic and antioxidant activities may provide a new approach in the treatment of diabetes mellitus.

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Inhibitory Properties of Ficus deltoidea on α-Glucosidase Activity

Cited by 23 publications

References 0 publications

Inhibitory Properties of Combined Ethanol Seed Extract of P. guineense and T. conophorum on -Glucosidase Enzyme

Inhibitory Properties of Combined Ethanol Seed Extract of P. guineense and T. conophorum on -Glucosidase Enzyme

Vitexin and isovitexin from the Leaves of Ficus deltoidea with in-vivo α-glucosidase inhibition

Antidiabetic and antioxidant properties of Ficus deltoidea fruit extracts and fractions

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