Inhomogeneities in sodium decylsulfate doped 1,2-dipalmitoylphosphatidylcholine bilayer

Ortona, Ornella; Vitagliano, Vincenzo; Fessas, Dimitrios; Vecchio, Pompea Del; D’Errico, Gerardino

doi:10.1016/j.jcis.2009.11.054

Cited by 5 publications

(4 citation statements)

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“…following the insulin secretory granules’ membrane paradigm . Indeed, the thermotropic behaviors of phospholipid vesicles and membranes have been shown to be severely influenced by the lipid composition as well as by the interaction of the bilayer with external agents. − …”

Section: Introductionmentioning

confidence: 99%

Influence of Free Fatty Acids on Lipid Membrane–Nisin Interaction

et al. 2020

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The influence of free fatty acids (FFAs) on the nisin–membrane interaction was investigated through micro-DSC and fluorescence spectroscopy. A simple but informative model membrane was prepared (5.7 DMPC:3.8 DPPS:0.5 DOPC molar ratio) by considering the presence of different phospholipid headgroups in charge and size and different phospholipid tails in length and unsaturation level, allowing the discrimination of the combined interaction of nisin and FFAs with the single phospholipid constituents. The effects of six FFAs on membrane stability were evaluated, namely two saturated FFAs (palmitic acid and stearic acid), two monounsaturated FFAs ( cis -unsaturated oleic acid and trans -unsaturated elaidic acid) and two cis -polyunsaturated FFAs (ω-6 linoleic acid and ω-3 docosahexaenoic acid). The results permitted assessment of a thermodynamic picture of such interactions which indicates that the peptide–membrane interaction does not overlook the presence of FFAs within the lipid bilayer since both FFAs and nisin are able to selectively promote thermodynamic phase separations as well as a general lipid reorganization within the host membrane. Furthermore, the magnitude of the effects may be different depending on the FFA chemical structure as well as the membrane lipid composition.

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Section: Introductionmentioning

confidence: 99%

Influence of Free Fatty Acids on Lipid Membrane–Nisin Interaction

et al. 2020

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“…Protein/peptide interactions with nucleic acids Lipid/membrane interactions Polysaccharide interactions …”

Section: Introductionmentioning

confidence: 99%

“…(vii) Lipid/membrane interactions. [325][326][327][328][329][330][331][332][333][334][335][336][337][338][339][340][341] (viii) Polysaccharide interactions. (ix) Protein/peptide interactions with polymers and nanoparticles.…”

Section: Introductionmentioning

confidence: 99%

Applications of isothermal titration calorimetry in pure and applied research—survey of the literature from 2010

Ghai

Falconer

Collins

2011

J of Molecular Recognition

167

108

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Isothermal titration calorimetry (ITC) is a biophysical technique for measuring the formation and dissociation of molecular complexes and has become an invaluable tool in many branches of science from cell biology to food chemistry. By measuring the heat absorbed or released during bond formation, ITC provides accurate, rapid, and label-free measurement of the thermodynamics of molecular interactions. In this review, we survey the recent literature reporting the use of ITC and have highlighted a number of interesting studies that provide a flavour of the diverse systems to which ITC can be applied. These include measurements of protein-protein and protein-membrane interactions required for macromolecular assembly, analysis of enzyme kinetics, experimental validation of molecular dynamics simulations, and even in manufacturing applications such as food science. Some highlights include studies of the biological complex formed by Staphylococcus aureus enterotoxin C3 and the murine T-cell receptor, the mechanism of membrane association of the Parkinson's disease-associated protein α-synuclein, and the role of non-specific tannin-protein interactions in the quality of different beverages. Recent developments in automation are overcoming limitations on throughput imposed by previous manual procedures and promise to greatly extend usefulness of ITC in the future. We also attempt to impart some practical advice for getting the most out of ITC data for those researchers less familiar with the method.

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“…In this process, surfactant molecules adsorb onto the liposome membrane, and are taken up into the membrane, and mixed micelles are subsequently emitted. These processes have been studied using methods including microscopy, 5 UV/Vis spectroscopy, [6][7][8] dynamic light scattering, 9,10 and cryo-TEM. [11][12][13] Many studies have been made on the dependence of solubilization on the surfactant and lipid concentrations; it is well understood that this occurs in an equilibrium state.…”

Section: Introductionmentioning

confidence: 99%