Initial Arthritic Lesions Induced by Immunization with Type II Collagen in Lewis Rats.

Takeshita, Masakazu; Hinoue, Hiromi; Furukawa, Osamu; Takata, Isao

doi:10.1538/expanim.44.63

Exp. Anim.

1995

DOI: 10.1538/expanim.44.63

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Initial Arthritic Lesions Induced by Immunization with Type II Collagen in Lewis Rats.

Masakazu Takeshita

Hiromi Hinoue

Osamu Furukawa

et al.

Abstract: Lewis rats were immunized with an intradermal injection of type II collagen to study the time course of arthritic lesions. Serum type II collagen antibody was detected 9 days after immunization. Increased paw volume in the hind limbs was noted on day 11. Histopathologically, proliferation of synovial lining cells was observed on day 11 and typical lesions similar to those of human rheumatoid arthritis were noted on day 18.

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Cited by 4 publications

(1 citation statement)

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“…Collagen-induced arthritis (CIA) is a model of experimental arthritis that is induced by the injection of type II collagen (CII) (2,3). The similarities between the joint pathology in CIA and RA (4) suggest that CIA is a relevant animal model that is useful in the search for new antiarthritis drugs (5)(6)(7)(8).…”

mentioning

confidence: 99%

Amelioration of joint disease in a rat model of collagen‐induced arthritis by M40403, a superoxide dismutase mimetic

Salvemini¹,

Mazzon

Dugo

et al. 2001

Arthritis & Rheumatism

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mentioning

confidence: 99%

Amelioration of joint disease in a rat model of collagen‐induced arthritis by M40403, a superoxide dismutase mimetic

Salvemini¹,

Mazzon

Dugo

et al. 2001

Arthritis & Rheumatism

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Liposome encapsulated aurothiomalate reduces collagen-induced arthritis in DBA/1J mice

Konigsberg

Debrick

Pawlowski

et al. 1999

Biochimica et Biophysica Acta (BBA) - Biomembranes

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Collagen-induced arthritis (CIA) generated in rats or mice has long been a model system for the study of rheumatoid arthritis in humans. In particular, this system has been used to study the mechanisms and effects of anti-arthritic drugs in the treatment of the disease. Sodium aurothiomalate (ATM) is an agent often used to treat rheumatoid arthritis in humans; however, it possesses inherent toxicities which limits its usefulness. Liposome-encapsulated drugs are currently being developed to minimize the toxicities associated with a variety of potentially beneficial drugs. We have chosen to encapsulate ATM into small unilamellar vesicles (SUVs) to determine whether greater efficacy would be achieved in treating CIA with SUV ATM as compared to using the free drug. SUVs were prepared from hydrogenated egg phosphatidylcholine and cholesterol. These SUVs were very stable. Vesicles stored at 4 degrees C lost only 0.09% of encapsulated ATM (SUV ATM) after 14 days and were able to reduce collagen-induced arthritis in these mice. Animals treated by i.m. injections of SUV ATM exhibited a 50% reduction in symptoms. More importantly, histological examination of knee joints of the affected animals verified that SUV ATM treatment prevented cellular infiltration of lymphocytes into the synovia of the collagen-sensitized mice. Conditioned media from spleen cell cultures was assayed for the presence of inflammatory lymphokines that might be affected by SUV ATM to account for the success in suppressing collagen-induced arthritis.

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Artemether: A New Therapeutic Strategy in Experimental Rheumatoid Arthritis

Cuzzocrea

Saadat

Paola

et al. 2005

Immunopharmacology and Immunotoxicology

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The current research was designed to determine the effect of artemether in treatment of experimental rheumatoid arthritis. Collagen-induced arthritis was induced in Lewis rats. The intramusculary administration of artemether (ART) and intraperitoneally injection of methotrexate (MTX) were started on day 25 postimmunization and continued until final assessment on day 35. During this period, clinical examination was taken intermittently. The anticollagen type II antibody (CII Ab) and nitric oxide synthesis were measured. The paws and kness were then removed for histopathology and radiography assay. The biocompatibility of ART and MTX were assessed using fibrosarcoma cell line. Data showed that i.m. injection of ART to arthritic rats induced a significant reduction in paw edema. This beneficial effect was associated with a significant decrease in anti-CII antibody response compared with untreated rats. Histopathological assessment showed a reduced inflammatory cell infiltrate in joints of treated rats; tissue edema, and bone erosion in the paws were markedly reduced following ART therapy. Furthermore, our radiography results paralleled our histological findings. Cytotoxicity analysis of ART showed greater tolerability compared with MTX. Treatment with ART significantly diminished NO formation in treated rats compared with nontreated controls. Our data shed light on the therapeutic efficacy of artemether in experimental rheumatoid arthritis compared with a choice drug (methotrexate), and it may be offered as a second-line drug in treatment of rheumatoid arthritis.

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Initial Arthritic Lesions Induced by Immunization with Type II Collagen in Lewis Rats.

Cited by 4 publications

References 5 publications

Amelioration of joint disease in a rat model of collagen‐induced arthritis by M40403, a superoxide dismutase mimetic

Amelioration of joint disease in a rat model of collagen‐induced arthritis by M40403, a superoxide dismutase mimetic

Liposome encapsulated aurothiomalate reduces collagen-induced arthritis in DBA/1J mice

Artemether: A New Therapeutic Strategy in Experimental Rheumatoid Arthritis

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