Initiation and characterization of five embryonic cell lines from the cotton boll weevil anthonomus grandis in a commercial serum- free medium

Evidence for the induction of cuticle proteins by 20‐hydroxyecdysone in two established insect cell lines

“…5h,i,k,l). In HE treated cells cytoplasmic inclusion bodies exhibited variable staining with the anti-CP antibodies (not shown) much as they did when stained in an earlier study [13] with wheat germ agglutinin and chitinase.…”

“…The two embryo-derived continuous insect cell lines, UM-BGE-4 from the German cockroach and the BRL-AG-2 line from the cotton boll weevil, both grow as cell aggregates or vesicles, both synthesize chitin and ecdysteroids, and are believed to be of epidermal origin [9,10,[13][14][15]263 We have generated mouse polyclonal antibodies which recognize the proteins extracted from the abdominal cuticle of various developmental stages of the German cockroach and the cotton boll weevil [27]. When Western blots of cell lysates were tested, these antibodies were observed to bind to three noninducible proteins from both of the cockroach cell lines and one noninducible protein from the boll weevil lines.…”

“…The cotton boll weevil lines BRL-AG-1, BRL-AG-3A, BRL-AG-3C, and BRL-AG-4, which grow as single attached cells, and line BRL-AG-2, which grows as cell aggregates and single attached cells, were maintained on Excel1 401 medium at 28°C [13].…”

“…Chitin synthesis may be induced or enhanced by ecdysteroids added to the cultures or by endogenous ecdysteroids produced by the cultured cells [10,11, and inhibited by several antibiotics and growth regulators [7,8,15]. Ecdysteroids have also been reported to cause other effects on cultured cells such as changes in morphology and growth patterns and the induction of protein and glycoprotein synthesis [17-251. We were interested in determining whether either of two continuous insect cell lines of presumed epidermal origin, the vesicle forming cockroach cell line UM-BGE-4 [26] and the cotton boll weevil aggregate forming line BRL-AG-2 [13], was capable of producing CP in addition to the chitin which both have been reported to synthesize [10,11,. And if so, was the CP synthesis regulated by the molting hormone, HE.…”

Cell-Based Screening Systems for Insecticides

“…This condition is not always satis fi ed, as illustrated by the Se4 (derived from the lepidopteran Spodoptera exigua ; Goodman et al 2001 ) and the coleopteran BCIRL-Lepd-SL1 (derived from the Colorado potato beetle Leptinotarsa decemlineata ; Long et al 2002 ) and BRL-AG-3A (derived from the cotton boll weevil Anthonomus grandis ; Stiles et al 1992 ) cell lines, which do not express reporter genes following transfection Soin et al 2009 ) . To explore alternative means of gene transduction in these cell lines, recombinant baculoviruses were generated that have incorporated the EcRE/b.act/GFP cassette .…”