Injectable adipose-derived stem cells-embedded alginate-gelatin microspheres prepared by electrospray for cartilage tissue regeneration

Liao, Song-Yan; Meng, Hui; Zhao, Jun; Lin, Wancheng; Liu, Xiuzhi; Tian, Zhigang; Lan, Lan; Yang, Hanyu; Zou, Yulan; Xu, Yingbin; Gao, Xiao; Lu, Shibi; Peng, Jiang

doi:10.1016/j.jot.2022.03.007

Cited by 12 publications

(15 citation statements)

References 41 publications

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“…Compared with traditional alginate microspheres, its support for ADSCs was better and demonstrated a better repair effect. This approach could be useful for cartilage tissue regeneration [ 73 ].…”

Section: Discussionmentioning

confidence: 99%

Intraarticular Injections of Mesenchymal Stem Cells in Knee Osteoarthritis: A Review of Their Current Molecular Mechanisms of Action and Their Efficacy

Rodríguez-Merchán

2022

IJMS

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More than 10% of the world’s population suffers from osteoarthritis (OA) of the knee, with a lifetime risk of 45%. Current treatments for knee OA pain are as follows: weight control; oral pharmacological treatment (non-steroidal anti-inflammatory drugs, paracetamol, opioids); mechanical aids (crutches, walkers, braces, orthotics); therapeutic physical exercise; and intraarticular injections of corticosteroids, hyaluronic acid, and platelet-rich plasma (PRP). The problem is that such treatments usually relieve joint pain for only a short period of time. With respect to intraarticular injections, corticosteroids relieve pain for several weeks, while hyaluronic acid and PRP relieve pain for several months. When the above treatments fail to control knee pain, total knee arthroplasty (TKA) is usually indicated; however, although a very effective surgical technique, it can be associated with medical and postoperative (surgery-related) complications. Therefore, it seems essential to look for safe and effective alternative treatments to TKA. Recently, there has been much research on intraarticular injections of mesenchymal stem cells (MSCs) for the management of OA of the knee joint. This article reviews the latest information on the molecular mechanisms of action of MSCs and their potential therapeutic benefit in clinical practice in patients with painful knee OA. Although most recent publications claim that intraarticular injections of MSCs relieve joint pain in the short term, their efficacy remains controversial given that the existing scientific information on MSCs is indecisive. Before recommending intraarticular MSCs injections routinely in patients with painful knee OA, more studies comparing MSCs with placebo are needed. Furthermore, a standard protocol for intraarticular injections of MSCs in knee OA is needed.

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Section: Discussionmentioning

confidence: 99%

Intraarticular Injections of Mesenchymal Stem Cells in Knee Osteoarthritis: A Review of Their Current Molecular Mechanisms of Action and Their Efficacy

Rodríguez-Merchán

2022

IJMS

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“…In this context, various hydrophilic polymers, including PLGA, PHA, and PCL, among others, have been employed to generate these solidified microarchitectures ( Hafeman et al, 2008 ; Wei et al, 2018 ; Liao et al, 2022 ). These solidified microparticles offer advantages of compatibility, excellent textural properties, and stability (thermal, colloidal, and suspension), which are of particular interest for minimally-invasive cell delivery towards TE applications ( Hafeman et al, 2008 ; Jiang et al, 2016 ).…”

Section: Classification Of Cell Carriersmentioning

confidence: 99%

“…These constructs showed promotion of osteo/odontogenic differentiation along with vascularization in the microcapsules, resulting in the deposited ECM. Similarly, Peng and colleagues utilized the electrospray technique to prepare alginate-gelatin microspheres embedded with adipose-derived stem cells for cartilage tissue regeneration ( Liao et al, 2022 ).…”

Section: Microfabrication Strategiesmentioning

confidence: 99%

“…To a considerable extent, the selection of polymer mainly plays a vital role in fabricating microcarriers for cell delivery. Based on the source of origination, different kinds of polymers have been employed to fabricate these 3D microcarriers (non-porous and porous) for cell delivery applications, such as natural (gelatin, dextran, cellulose, chitin and its derivatives, and alginic acid) ( Bidarra et al, 2014 ; Ho et al, 2020 ; Lavanya et al, 2020 ; Rahman et al, 2021 ; Liang et al, 2022 ; Liao et al, 2022 ), and synthetic (PLGA, PLA, silk fibroin, SF, polyurethane, polyacrylamide, and PHEMA) ( Hong et al, 2005 ; Hafeman et al, 2008 ; Braccini et al, 2020 ). Considering the pros and cons, polymers from natural sources possess high biocompatibility due to biomolecules, recyclability, and mechanical properties ( Rahman et al, 2021 ).…”

Section: Factors Influencing Cell Deliverymentioning

confidence: 99%

“…In most of the instances, several polymers with compatibility have been demonstrated, for instance, natural [chitosan ( Huang et al, 2018 ), HA ( Bae et al, 2006 ), and alginate ( Chen et al, 2015 )], and synthetic [PLGA ( Kankala et al, 2019 ), PLA ( Liu et al, 2011 ), and PCL ( Kim et al, 2016 )], as well as their mixture ( Hong et al, 2005 ). In this framework, several cell lines from humans and mice have been encapsulated to demonstrate the potential of the microcarriers, such as osteoblasts ( Chen et al, 2015 ), skeletal myoblasts ( Kankala et al, 2019 ), chondrocytes ( Hong et al, 2005 ; Liu et al, 2011 ), and MSCs ( Liu et al, 2011 ; Barati et al, 2017 ; Luetchford et al, 2020 ; Liao et al, 2022 ), indicating their biocompatibility due to viability and proliferation abilities ( Ho et al, 2020 ). Although these carriers showed compatibility with different cell lines, comprehensive toxicity evaluations must be systematically evaluated, including the genotoxicity and other toxicity evaluations.…”

Section: Scope For Preclinical/clinical Applicabilitymentioning

confidence: 99%

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Polymeric microcarriers for minimally-invasive cell delivery

Duan

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et al. 2023

Front. Bioeng. Biotechnol.

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Tissue engineering (TE) aims at restoring tissue defects by applying the three-dimensional (3D) biomimetic pre-formed scaffolds to restore, maintain, and enhance tissue growth. Broadly speaking, this approach has created a potential impact in anticipating organ-building, which could reduce the need for organ replacement therapy. However, the implantation of such cell-laden biomimetic constructs based on substantial open surgeries often results in severe inflammatory reactions at the incision site, leading to the generation of a harsh adverse environment where cell survival is low. To overcome such limitations, micro-sized injectable modularized units based on various biofabrication approaches as ideal delivery vehicles for cells and various growth factors have garnered compelling interest owing to their minimally-invasive nature, ease of packing cells, and improved cell retention efficacy. Several advancements have been made in fabricating various 3D biomimetic microscale carriers for cell delivery applications. In this review, we explicitly discuss the progress of the microscale cell carriers that potentially pushed the borders of TE, highlighting their design, ability to deliver cells and substantial tissue growth in situ and in vivo from different viewpoints of materials chemistry and biology. Finally, we summarize the perspectives highlighting current challenges and expanding opportunities of these innovative carriers.

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Vanillin-based functionalization strategy to construct multifunctional microspheres for treating inflammation and regenerating intervertebral disc

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et al. 2023

Bioactive Materials

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Injectable adipose-derived stem cells-embedded alginate-gelatin microspheres prepared by electrospray for cartilage tissue regeneration

Cited by 12 publications

References 41 publications

Intraarticular Injections of Mesenchymal Stem Cells in Knee Osteoarthritis: A Review of Their Current Molecular Mechanisms of Action and Their Efficacy

Intraarticular Injections of Mesenchymal Stem Cells in Knee Osteoarthritis: A Review of Their Current Molecular Mechanisms of Action and Their Efficacy

Polymeric microcarriers for minimally-invasive cell delivery

Vanillin-based functionalization strategy to construct multifunctional microspheres for treating inflammation and regenerating intervertebral disc

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