Injectable and Biocompatible Mussel‐Inspired Adhesive for Enhanced Reendothelialization of Injured Artery

Xu, Chengyuan; Yang, Czau‐Siung; Si, Yi; Yan, Yunfeng; Kayitmazer, A. Basak; Ding, Yong; Qian, Weiwei; Zheng, Zhiyuan; Cao, Song; Xu, Yisheng

doi:10.1002/admi.202001955

Cited by 8 publications

(4 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The SMCs have relevance, as successful adhesion would be an initial indication of a potential use wherein the polymer could be used to patch arteries without the need of stiches. 52 The EG7 immortalized line of T lymphocyte cells are often used as a model system for studying major histocompatibility complex class I restricted responses of cytotoxic T lymphocytes. For that purpose, these cells have to be immobilized, which is currently done using harmful reagents such as aldehydes.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Mussel Adhesive-Inspired Proteomimetic Polymer

et al. 2022

View full text Add to dashboard Cite

Herein, a synthetic polymer proteomimetic is described that reconstitutes the key structural elements and function of mussel adhesive protein. The proteomimetic was prepared via graft-through ring-opening metathesis polymerization of a norbornenyl-peptide monomer. The peptide was derived from the natural underwater glue produced by marine mussels that is composed of a highly repetitive 10 amino acid tandem repeat sequence. The hypothesis was that recapitulation of the repeating unit in this manner would provide a facile route to a nature-inspired adhesive. To this end, the material, in which the arrangement of peptide units was as side chains on a brush polymer rather than in a linear fashion as in the natural protein, was examined and compared to the native protein. Mechanical measurements of adhesion forces between solid surfaces revealed improved adhesion properties over the natural protein, making this strategy attractive for diverse applications. One such application is demonstrated, using the polymers as a surface adhesive for the immobilization of live cells.

show abstract

Section: ■ Results and Discussionmentioning

confidence: 99%

Mussel Adhesive-Inspired Proteomimetic Polymer

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Commercial adhesives such as Loctite superglue and Braun Histoacryl with cyanoacrylates were employed as comparisons. [ 36 ] The conditioned media from both Loctite superglue and Braun Histoacryl were found to be very cytotoxic to the cells, with an average viability of 21% and 14%, respectively, Figure A. The cytotoxicity of the Loctite superglue was concentration dependent as a 4× dilution showed little effect on cell viability and a 20× dilution had no impact.…”

Section: Biocompatibilitymentioning

confidence: 99%

Efficient Wet Adhesion through Mussel‐Inspired Proto‐Coacervates

Huynh

Chang

Bach-Gansmo

et al. 2022

Adv Materials Inter

View full text Add to dashboard Cite

threads was generated by secreting liquid mussel foot proteins (Mfps) from mussel foot. These Mfps are assembled and manufactured by glands by injection molding reaction. [3] The foot of a mussel presses against the surface to create a vacuum chamber, which propels the delivery of fluidic Mfps. It is believed that Mfps confined to plaques, such as Mfp-2, Mfp-3, Mfp-4, and Mfp-5, create coacervates when exposed to saltwater. All mfps include the post-translational amino acid DOPA, and mfp-5 contains the largest concentration of DOPA residues (30 mol%) and leads to strong adhesion. [4] It was reported that the coacervation of mfps, which occurs in a variety of ways, such as complex coacervation driven by electrostatic interaction, as revealed in polyions of Mfp-131 and Mfp-151, [5] and self-coacervation driven by electrostatic and/or hydrophobic forces as revealed in Mfp-3S. [6] An essential component in mussel adhesion is the special amino acid L-DOPA with its catechol group. [7] The catechols act as chemical jack-of-all-trades or Janus-like chemicals and allow attachment to almost all types of material surfaces using either covalent or noncovalent bonds. [8] Several research groups have suggested mussel-inspired materials to be used as bio-inspired adhesives, [9] with efforts being made by including features of the mussel foot proteins in synthetic polymers. [8a,b,10] Hence, catechols and analogues thereof are highly relevant in materials design. [8e,g] Great strides have been made in harnessing mussel-inspired chemistry in materials, however, many of previous efforts lack a simple and efficient way to deliver the materials to a surface underwater for efficient adhesion. [11] The blue mussel is thought to deliver its adhesive in the form of complex fluids that spread spontaneously and exhibit strong reversible interfacial bonding and tunable cross-linking. [4] The complex fluids are coacervates [12] -mainly composing mixtures of polyelectrolytes that form a separate phase from the aqueous medium. Thus, the mussel maintains its complex fluid glue that upon meeting the surface, spreads and presents DOPA residues to the target surface for attachment and subsequent curing (Figure 1A,B). Indeed, coacervates are used widely in biology (Figure 1A), including in sandcastle worm adhesives [13] and to infiltrate squid beak scaffold materials with proteins. [14] In the blue mussel, the whole gluing process takes only a few minutes and occurs within the spatial confines of the byssus Adhesion underwater is a major challenge. Mussel-inspired complex coacervates functionalized with L-3,4-dihydroxyphenylalanine (L-DOPA) are proposed for underwater adhesives through versatile chemistry of DOPA, however, simple, efficient, controllable, and nontoxic procedures to harness them are still under investigation. In this study, inspired from the mussel byssus formation process, coacervate adhesives are formed underwater by simple injection of an acidic proto-coacervate of DOPA functionalized polyelectrolytes on underwater surface...

show abstract

“…[29] This intriguing biological material prompts to pursue advanced multi-functional adhesive materials. [9,[30][31][32][33][34][35][36][37][38][39] Due to the high availability, biocompatibility, and good mechanical properties, [40,41] cellulose and cellulose derivatives have been widely used in various fields such as hydrogels, paper, aerogel, membranes, medicines, sorption agents, and coatings. [42][43][44][45][46][47][48][49][50] The hydroxyl groups on anhydroglucose units make cellulose suitable for a variety of physical or chemical modifications.…”

Section: Introductionmentioning

confidence: 99%

“…[ 29 ] This intriguing biological material prompts to pursue advanced multi‐functional adhesive materials. [ 9,30–39 ]…”

Section: Introductionmentioning

confidence: 99%

Biocompatible Catechol‐Functionalized Cellulose‐Based Adhesives with Strong Water Resistance

Tang

Bian

Lin

et al. 2021

Macro Materials & Eng

View full text Add to dashboard Cite

Numerous traditional adhesives have good adhesion in dry environments. However, non-environmental-friendliness and poor water resistance largely limit their practical applications. To prepare biocompatible adhesives with strong water resistance and adhesion strength, in this paper, catechol-functionalized cellulose-based adhesive polymers are synthesized by grafting N-(3,4-dihydroxyphenethyl)methacrylamide and methyl methacrylate onto cellulose chain through atom transfer radical polymerization (ATRP). The successful synthesis of the catechol-functionalized cellulose-based adhesive polymers is confirmed by FTIR and 1 H NMR. The different characteristics of the adhesive polymers, such as thermal stability, swelling ratio, biocompatibility, and adhesion strength are investigated. Strong water resistance on various substrates is realized in underwater environment for the catechol-functionalized cellulose-based adhesive with addition of Fe 3+ . The adhesion strength and thermal stability are enhanced when the catechol content is increased. The adhesive with catechol content of 25.4% shows the adhesion strength of 0.45 MPa for iron substrate in underwater environment. In addition, the adhesive with addition of Fe 3+ exhibits excellent adhesion in dry environment, with maximum adhesion strength of 3.50 MPa for iron substrate. The cell culture test shows that the adhesive polymers have excellent biocompatibility. The biocompatible adhesives with strong water resistance have potential application in electronic, wood, and building fields.

show abstract

Injectable and Biocompatible Mussel‐Inspired Adhesive for Enhanced Reendothelialization of Injured Artery

Cited by 8 publications

References 56 publications

Mussel Adhesive-Inspired Proteomimetic Polymer

Mussel Adhesive-Inspired Proteomimetic Polymer

Efficient Wet Adhesion through Mussel‐Inspired Proto‐Coacervates

Biocompatible Catechol‐Functionalized Cellulose‐Based Adhesives with Strong Water Resistance

Contact Info

Product

Resources

About