Injectable Collagen Implant Improves Survival, Cardiac Remodeling, and Function in the Early Period After Myocarditis in Rats

Abstract

The present study shows, for the first time, that injectable collagen biomaterial improves survival and attenuates cardiac inflammation, cardiomyocyte hypertrophy, LV remodeling, and dysfunction in the early period after myocarditis in rats. Our findings suggest a new biomaterial-based strategy to ameliorate the devastating effects of myocarditis.

VA‐086 methacrylate gelatine photopolymerizable hydrogels: A parametric study for highly biocompatible 3D cell embedding

“…In this regard, considering the wide range of polymeric precursors and crosslinking methods used to fabricate biomimetic hydrogels, systematic studies aiming at elucidating the influence of all involved parameters on hydrogel properties are still required to fully exploit their potential in tissues replication. To date, several polymerization strategies have been successfully investigated, either for fabricating 3D scaffolds for further cell seeding or directly for 3D cells embedding within matrices . Among these strategies, photopolymerization has been widely investigated and demonstrated to be a promising approach to obtain highly viable 3D cellular matrices .…”

“…Most chemical methods are not compatible with 3D encapsulation of viable cells, usually relying on cytotoxic chemicals or enzymatic agents to trigger the polymerization . Thermal reticulation has been widely investigated as a biocompatible process for creating cell‐laden hydrogels; however, the presence of living cells poses an upper temperature threshold to 37°C, generally resulting in relatively long crosslinking times. For this reason, additional rotating systems may be required to thermally crosslink cell‐laden hydrogels in order to prevent cell tendency to settle down and thus achieve a uniform 3D cell distribution within the matrix .…”

Preparation, characterization and biocompatibility of calcium peroxide-loaded polycaprolactone microparticles

“…评价材料的生物相容性应遵循生物安全性和生物功能性两个原则，既要求生物材料具有很低的毒性，同时要求生物材料在特定的应用中能够恰当地激发机体相应的功能 ［ 12 - 13 ］ 。本文资料显示，CPO/PCL植入组织7 d时，微粒并没有融入组织，微粒周边组织空泡化严重，呈大面积空泡化，而注射14 d时肌肉纤维排列较为整齐，间隙大小未增厚，可见稍许充血，局部炎症细胞浸润，未见明显空泡化现象。可知在CPO/PCL植入组织后前期会对周边组织造成一定损伤，该现象在一定时间后可以得到改善，需要一定的时间才能与组织较好融合。另外，生物材料的植入对组织造成的损伤体现在炎症的慢性变化以及自身免疫的表达，在此过程中起主要作用的是巨噬细胞和T淋巴细胞 ［ 14 - 16 ］ 。CD3和CD68是常用于组织损伤检查的指标。本文资料显示，CPO/PCL微粒植入14 d时，肌肉组织CD3和CD68阳性细胞表达较7 d时明显减少，提示CPO/PCL微粒对周边组织的炎症及免疫反应的影响较小，综合病理观察结果可以认为该生物材料具有较好的生物相容性。另外，CPO/PCL微粒对脂肪间充质干细胞中 Alp 、 Runx2 、 Ocn 和 Opn 基因表达有促进作用，初步认为其可以促进间充质干细胞成骨分化。…”

Improving the development of human engineered cardiac tissue by gold nanorods embedded extracellular matrix for long-term viability

“…16 Moreover, the mechanical properties of the hydrogels can be precisely tuned to influence the biological function of CMs, thus offering additional control over the regulation process. 17 Various naturally-derived biomaterials, including fibrin, 18 collagen, 19,20 gelatin 21 and extracellular matrix (ECM)-based materials, have been widely used to enhance retention, survival and engraftment of tissue constructs. These biomaterials serve as scaffolding to facilitate revascularization.…”