Injectable ECM hydrogel for delivery of BMSCs enabled full-thickness meniscus repair in an orthotopic rat model

Zhong, Gang; Yao, Jun; Huang, Xing; Luo, Yixuan; Wang, Meng; Han, Jianming; Chen, Fei; Yu, Yancun

doi:10.1016/j.bioactmat.2020.06.008

Cited by 63 publications

(58 citation statements)

References 30 publications

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“…Similar results have been reported before in the literature with a range of tissue grafts, cell populations and preclinical models (e.g. hADSC delivered by a porcine small intestinal submucosa graft to a rat ventral model [49], hADSC delivered by a decellularised porcine nucleus pulposus device to a rabbit intervertebral disc degeneration model [50], rat bone marrow stem cells delivered by a porcine decellularised meniscus materials to a full-thickness rat meniscus defect model [51]). The gradual loss in fluorescent signal observed in all conditions could be also attributed to the loss of scar tissue after 2 weeks, as has been suggested before in the same model [52,53].…”

Section: Discussionsupporting

confidence: 86%

Extracellular matrix-based biomaterials as adipose-derived stem cell delivery vehicles in wound healing: a comparative study between a collagen scaffold and two xenografts

et al. 2020

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Background Stem cell therapies represent a promising tool in regenerative medicine. Considering the drawbacks of direct stem cell injections (e.g. poor cell localisation), extracellular matrix-based biomaterials (e.g. scaffolds and tissue grafts), due to their compositional biofunctionality and cytocompatibility, are under investigation as potential stem cell carriers. Methods The present study assessed the potential of three commercially available extracellular matrix-based biomaterials [a collagen/glycosaminoglycan scaffold (Integra™ Matrix Wound Dressing), a decellularised porcine peritoneum (XenoMEM™) and a porcine urinary bladder (MatriStem™)] as human adipose-derived stem cell delivery vehicles. Results Both tissue grafts induced significantly (p < 0.01) higher human adipose-derived stem cell proliferation in vitro over the collagen scaffold, especially when the cells were seeded on the basement membrane side. Human adipose-derived stem cell phenotype and trilineage differentiation potential was preserved in all biomaterials. In a splinted wound healing nude mouse model, in comparison to sham, biomaterials alone and cells alone groups, all biomaterials seeded with human adipose-derived stem cells showed a moderate improvement of wound closure, a significantly (p < 0.05) lower wound gap and scar index and a significantly (p < 0.05) higher proportion of mature collagen deposition and angiogenesis (the highest, p < 0.01, was observed for the cell loaded at the basement membrane XenoMEM™ group). All cell-loaded biomaterial groups retained more cells at the implantation side than the direct injection group, even though they were loaded with half of the cells than the cell injection group. Conclusions This study further advocates the use of extracellular matrix-based biomaterials (in particular porcine peritoneum) as human adipose-derived stem cell delivery vehicles. Graphical abstract Comparative analysis of a collagen scaffold (Integra™ Matrix Wound Dressing) and two tissue grafts [decellularised porcine peritoneum (XenoMEM™) and porcine urinary bladder (MatriStem™)] as human adipose-derived stem cells carriers

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Section: Discussionsupporting

confidence: 86%

Extracellular matrix-based biomaterials as adipose-derived stem cell delivery vehicles in wound healing: a comparative study between a collagen scaffold and two xenografts

et al. 2020

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“…In recent years, injectable hydrogels have been developed with excellent biocompatibility and controlled drug delivery behaviors in tissue engineering [ [7] , [8] , [9] ]. For example, Xin et al .…”

Section: Introductionmentioning

confidence: 99%

Biomimetic injectable hydrogel microspheres with enhanced lubrication and controllable drug release for the treatment of osteoarthritis

Han

Yang

Zhao

et al. 2021

Bioactive Materials

163

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The occurrence of osteoarthritis (OA) is highly associated with the reduced lubrication property of the joint, where a progressive and irreversible damage of the articular cartilage and consecutive inflammatory response dominate the mechanism. In this study, bioinspired by the super-lubrication property of cartilage and catecholamine chemistry of mussel, we successfully developed injectable hydrogel microspheres with enhanced lubrication and controllable drug release for OA treatment. Particularly, the lubricating microspheres (GelMA@DMA-MPC) were fabricated by dip coating a self-adhesive polymer (DMA-MPC, synthesized by free radical copolymerization) on superficial surface of photo-crosslinked methacrylate gelatin hydrogel microspheres (GelMA, prepared via microfluidic technology), and encapsulated with an anti-inflammatory drug of diclofenac sodium (DS) to achieve the dual-functional performance. The tribological test and drug release test showed the enhanced lubrication and sustained drug release of the GelMA@DMA-MPC microspheres. In addition, the functionalized microspheres were intra-articularly injected into the rat knee joint with an OA model, and the biological tests including qRT-PCR, immunofluorescence staining assay, X-ray radiography and histological staining assay all revealed that the biocompatible microspheres provided significant therapeutic effect against the development of OA. In summary, the injectable hydrogel microspheres developed herein greatly improved lubrication and achieved sustained local drug release, therefore representing a facile and promising technique for the treatment of OA.

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“…Hydrogels with a high percentage of water in their three-dimensional network have been shown to mimic the extracellular matrix (ECM) better [ [1] , [2] , [3] ]. They play an essential role in the development of tissue engineering scaffolds because of their ability to provide microenvironments for cell adhesion, proliferation and migration, and to promote the exchange of biomolecules, while not affecting their biological activity [ 4 , 5 ]. The mechanical parameters of hydrogel scaffolds will be sensed by cells and affect the cell behavior [ [6] , [7] , [8] ].…”

Section: Introductionmentioning

confidence: 99%

Mussel-inspired agarose hydrogel scaffolds for skin tissue engineering

Zhang

Zeng

et al. 2021

Bioactive Materials

165

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Polysaccharide hydrogels are widely used in tissue engineering because of their superior biocompatibility and low immunogenicity. However, many of these hydrogels are unrealistic for practical applications as the cost of raw materials is high, and the fabrication steps are tedious. This study focuses on the facile fabrication and optimization of agarose-polydopamine hydrogel (APG) scaffolds for skin wound healing. The first study objective was to evaluate the effects of polydopamine (PDA) on the mechanical properties, water holding capacity and cell adhesiveness of APG. We observed that APG showed decreased rigidity and increased water content with the addition of PDA. Most importantly, decreased rigidity translated into significant increase in cell adhesiveness. Next, the slow biodegradability and high biocompatibility of APG with the highest PDA content (APG3) was confirmed. In addition, APG3 promoted full-thickness skin defect healing by accelerating collagen deposition and promoting angiogenesis. Altogether, we have developed a straightforward and efficient strategy to construct functional APG scaffold for skin tissue engineering, which has translation potentials in clinical practice.

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Injectable ECM hydrogel for delivery of BMSCs enabled full-thickness meniscus repair in an orthotopic rat model

Cited by 63 publications

References 30 publications

Extracellular matrix-based biomaterials as adipose-derived stem cell delivery vehicles in wound healing: a comparative study between a collagen scaffold and two xenografts

Extracellular matrix-based biomaterials as adipose-derived stem cell delivery vehicles in wound healing: a comparative study between a collagen scaffold and two xenografts

Biomimetic injectable hydrogel microspheres with enhanced lubrication and controllable drug release for the treatment of osteoarthritis

Mussel-inspired agarose hydrogel scaffolds for skin tissue engineering

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