Injectable Mussel‐Inspired Immobilization of Platelet‐Rich Plasma on Microspheres Bridging Adipose Micro‐Tissues to Improve Autologous Fat Transplantation by Controlling Release of PDGF and VEGF, Angiogenesis, Stem Cell Migration

Zhou, Shaolong; Chang, Qiang; Lu, Feng; Xing, Malcolm

doi:10.1002/adhm.201700131

Cited by 37 publications

(29 citation statements)

References 73 publications

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“…Hence, PRP has been used for fat and skin repair and as an adhesive for wound closure. It has, for example, been shown that PRP (mean enrichment 6.4-fold) immobilized on gelatin microspheres improved the engraftment and particularly the vascularization of transplanted fat upon subcutaneous implantation [53]. Promising results were also reported for the application of a mixture of PRP with heparin-conjugated fibrin for the treatment of full-thickness wounds in mice.…”

Section: Prp In Tissue-engineered Constructsmentioning

confidence: 71%

Platelet-Rich Plasma in Tissue Engineering: Hype and Hope

Lang

Loibl²,

Herrmann³

2018

Eur Surg Res

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Background: Platelet-rich plasma (PRP) refers to an enriched platelet suspension in plasma. In addition to the clinical application of PRP in the context of various orthopedic diseases and beyond, PRP and platelet lysate (PL) have been in focus in the field of tissue engineering. In this review, we discuss the application of PRP as a cell culture supplement and as part of tissue engineering strategies, particularly emphasizing current hurdles and ambiguities regarding the efficacy of PRP in these approaches. Summary: As a putative autologous replacement for animal-derived supplements such as fetal calf serum (FCS), PRP has been applied as cell culture supplement for the expansion of stem and progenitor cells for tissue engineering applications and cell therapies. Attributed to the high content of growth factors in platelets, PRP has been shown to promote cell growth, which was mostly superior to standard cultures supplemented with FCS, while the differentiation capacity of progenitor cells seems not to be affected. However, it was also suggested that cultivation of cells with PRP significantly alters the protein expression profile in cells in comparison to FCS, indicating that the influence of PRP on cell behavior should be thoroughly investigated. Moreover, different PRP preparation methods and donor variations have to be considered for the use of PRP under good manufacturing practice conditions. PRP has been used for various tissue engineering applications in the context of bone, cartilage, skin, and soft tissue repair, where most studies were conducted in the field of bone tissue engineering. These approaches take either advantage of the release of chemoattractive, angiogenic, proliferative, and putatively pro-regenerative growth factors from PRP, and/or the hydrogel properties of activated PRP, making it suitable as a cell delivery vehicle. In many of these studies, PRP is combined with biomaterials, cells, and in some cases recombinant growth factors. Although the experimental design often does not allow conclusions on the pro-regenerative effect of PRP itself, most publications report beneficial effects if PRP is added to the tissue-engineered construct. Furthermore, it was demonstrated that the release of growth factors from PRP may be tailored and controlled when PRP is combined with materials able to capture growth factors. Key Messages: Platelet-derived preparations such as PRP and PL represent a promising source of autologous growth factors, which may be applied as cell culture supplement or to promote regeneration in tissue-engineered constructs. Furthermore, activated PRP is a promising candidate as an autologous cell carrier. However, the studies investigating PRP in these contexts often show conflicting results, which most likely can be attributed to the lack of standardized preparation methods, particularly with regard to the platelet content and donor variation of PRP. Ultimately, the use of PRP has to be tailored for the individual application.

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Section: Prp In Tissue-engineered Constructsmentioning

confidence: 71%

Platelet-Rich Plasma in Tissue Engineering: Hype and Hope

Lang

Loibl²,

Herrmann³

2018

Eur Surg Res

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“…Lots of basic science studies on cell cultures and animal models as well as human clinical trials supported the application of PRP. [26][27][28] Numbers studies have focused on its effect on multiple stem cells proliferation, [29][30][31][32] EnMSCs could be conveniently segregated from menstrual blood and expanded to create almost unlimited supplies. There are several advantages of EnMSCs, including embryoniclike properties, noninvasive isolation procedures, and improved function.…”

Section: Discussionmentioning

confidence: 99%

Investigation of platelet‐rich plasma in increasing proliferation and migration of endometrial mesenchymal stem cells and improving pregnancy outcome of patients with thin endometrium

Wang

Liu

Mou

et al. 2018

J of Cellular Biochemistry

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Background Platelet‐rich plasma (PRP) contains abundant growth factors and is gradually used in the field of reproduction. A thin endometrium is recognized as a critical factor in embryo implantation failure. Endometrial mesenchymal stem cells (EnMSCs), which were isolated from human menstrual blood, are highly proliferative and show multiple differentiation capacity. The current study was to investigate the effect of PRP on the proliferation and migration of EnMSCs, and the effectiveness of PRP in the treatment of patients with thin endometrium. Materials and Methods EnMSCs were treated with PRP in vitro, followed by measuring cell proliferation, migration, and adhesion by using CCK8, scratch, and adhesion test, respectively. Twenty patients undergoing in vitro fertilization (IVF) with refractory thin endometrium history were given PRP by infusion into the uterine cavity after the treatment of hormone replacement therapy (HRT). Results All components of PRP significantly stimulated the growth, migration, and adhesion of EnMSCs when compared with the negative control. Cell proliferation and migration were induced by PRP in a dose‐dependent manner with maximum proliferation at a 2% PRP dose. The clinical data showed that successful endometrial expansion and pregnancy were discovered in 12 patients after PRP infusion, and the pregnancy rate increased to 60%. Conclusion Intrauterine PRP infusion represents a new way for female patients with thin endometrium with poor response. This study lays the foundations for the potential treatment of thin endometrium with PRP in vivo.

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“…Previous studies have veried that various biomolecules can easily conjugate onto the surface of the PDA-coated materials, depending on the reaction of catechols with thiols and amines, such as BMP-2, VEGF and bFGF. 26,27,34 Zhou et al successfully immobilized P24 peptide on polydopamine coated nHA/RHLC/PLA scaffolds and found that PDA-assisted coating could effectively immobilize P24 peptide on the surface of nHA/RHLC/PLA scaffold and allowed for its controlled release. 35 Another study found that immobilized bFGF on PDA coated PLGA electrospun brous scaffolds showed higher bFGF immobilization efficiency than PLGA electrospun brous scaffolds.…”

Section: Adsorption and Release Of Igf-1 And Bmp-2mentioning

confidence: 99%