Injectable silk fibroin/polyurethane composite hydrogel for nucleus pulposus replacement

Hu, Jingen; Chen, Bin; Guo, Fei; Du, Jinlin; Gu, Pengcheng; Lin, Xianfu; Yang, Weiping; Zhang, Hailong; Lu, Min; Huang, Yiping; Ge-wen, XU

doi:10.1007/s10856-011-4533-y

Cited by 60 publications

(45 citation statements)

References 39 publications

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“…for bone repair (Hardy et al, 2015;Li et al, 2006;Park et al, 2010;Sofia et al, 2001;Zhang et al, 2014), for artificial skin (Sheikh et al, 2015), vascular grafts (Catto et al, 2015;Wang et al, 2015;Zhang et al, 2008), substrate for growing neuronal cells (Sun et al, 2015), IVD repair (Buser et al, 2011;Chang et al, 2010;Chen et al, 2015;Hu et al, 2012;Park et al, 2011;Zeng et al, 2014;Web Ref.1) (Table 2) and cartilage repair Hofmann et al, 2006a;Wang et al, 2005). Additionally, silk can also be used as a drug delivery platform (Mwangi et al, 2015;Qu et al, 2014) for antibiotics (Pritchard et al, 2013) and proteins or small molecule drugs (Meinel and Kaplan, 2012).…”

Section: Silk For Tissue Engineering Backgroundmentioning

confidence: 99%

A review of the application of reinforced hydrogels and silk as biomaterials for intervertebral disc repair

Frauchiger¹,

Tekari²,

Wöltje³

et al. 2017

eCM

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The degeneration of the intervertebral disc (IVD) within the spinal column represents a major pain source for many patients. Biological restoration or repair of the IVD using "compressive-force-resistant" and at the same time "cytocompatible" materials would be desirable over current purely mechanical solutions, such as spinal fusion or IVD implants. This review provides an overview of recent research on the repair of the inner (nucleus pulposus = NP) and the outer (annulus fibrous = AF) parts of the IVD tissue. Many studies have addressed NP repair using hydrogel-like materials. However, only a few studies have so far focused on AF repair. As the AF possesses an extremely low self-healing capacity and special attention to shear-force resistance is essential, special repair designs are required. In our review, we stated the challenges in IVD repair and highlighted the use of composite materials such as silk biomaterials and fibrin cross-linked reinforced hydrogels. We elaborated on the origin of silk and its many in tissue engineering. Furthermore, techniques such as electrospinning and 3D printing technologies allow the fabrication of versatile and functionalised 3D scaffolds. We summarised the research that has been conducted in the field of regenerative medicine over the recent years, with a special focus on the potential application and the potential of combining silk and reinforced -and thus mechanically tailored -hydrogels for IVD repair.

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Section: Silk For Tissue Engineering Backgroundmentioning

confidence: 99%

A review of the application of reinforced hydrogels and silk as biomaterials for intervertebral disc repair

Frauchiger¹,

Tekari²,

Wöltje³

et al. 2017

eCM

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“…To obtain the fibroin, first, we prepared the silkworm cocoons, which we cut into small pieces and then submerged them in an aqueous solution 0.1% wt/v of NaCO 3 at 80 °C for one hour under magnetic stirring to remove the sericin [17,18]. Subsequently, we washed the SF extract with distilled water and dissolved it in a solution of CaCl 2 , H 2 O and CH 3 CH 2 OH 1:8:2 molar between 45 °C and 55 °C for one hour [19][20][21].…”

Section: B Fibroin (Sf) Preparation and Characterizationmentioning

confidence: 99%

Degradation, water uptake, injectability and mechanical strength of injectable bone substitutes composed of silk fibroin and hydroxyapatite nanorods

Buitrago-Vásquez

Orozco

2018

Rev. Fac. Ing.

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Injectable bone substitutes are generally composite materials capable of being extruded through a device; they consist of a solid phase inside a matrix that allows the easy movement of particles. Injectable bone substitutes have the advantage of allowing its application in situ without the need of invasive surgical techniques, guaranteeing a good recovery; for this reason, they are a promising alternative to replace conventional techniques to repair bone defects. Conventional techniques include the use of allografts and autografts, which often cause adverse reactions, and are disadvantageous for both the patient and the doctor. Furthermore, there are no reports regarding bone substitute development in Colombia, creating the necessity to research composite materials that could become injectable bone substitutes. In this study, we manufactured injectable bone substitutes with hydroxyapatite and calcium phosphate, which is most similar to bone tissue, and synthesized them in nanorods with shape and size similar to the natural hydroxyapatite found inside the body. Additionally, we used extracted silk fibroin from silkworm cocoons of Bombyx mori, a natural polymer of protein nature with high mechanical properties and excellent biocompatibility. For the materials manufactured, we evaluated degradation, in a simulated body fluid (SBF) at normal body temperature, water uptake, injectability and mechanical strength. The manufactured bone substitutes showed good degradation and water uptake properties, an approximate 97% injectability, and low mechanical resistance, indicating promising properties to be used as an injectable bone substitute.

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“…Little NP cell research. Attia et al, 2011;Dahl et al, 2010;Hu et al, 2012;Mauth et al, 2009;Park et al, 2013;van Tienen et al, 2009;Santerre et al, 2005;Yeganegi et al, 2010 …”

Section: Vascular Graftsunclassified

“…Various PU composite scaffolds have been created, with PU/silk fibroin (SF) hydrogels as the main example (Hu et al, 2012;Park et al, 2013). The addition of silk to PU scaffolds provides control over the degradation rate and mechanical properties.…”

Section: Polyurethanementioning

confidence: 99%

“…The addition of silk to PU scaffolds provides control over the degradation rate and mechanical properties. PU/SF scaffolds were used to replace the NP in cadaveric porcine spines by injection into the NP cavity (Hu et al, 2012). To scaffolds made of electrospun PU, AF cells attached and produced ECM components (Yeganegi et al, 2010;Attia et al, 2011).…”

Section: Polyurethanementioning

confidence: 99%

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Biomaterials for intervertebral disc regeneration: past performance and possible future strategies

Schutgens¹,

Tryfonidou²,

Smit³

et al. 2015

eCM

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Intervertebral disc (IVD) degeneration is associated with most cases of cervical and lumbar spine pathologies, amongst which chronic low back pain has become the number one cause of loss of quality-adjusted life years. In search of alternatives to the current less than optimal and usually highly invasive treatments, regenerative strategies are being devised, none of which has reached clinical practice as yet. Strategies include the use of stem cells, gene therapy, growth factors and biomaterial carriers. Biomaterial carriers are an important component in musculoskeletal regenerative medicine techniques. Several biomaterials, both from natural and synthetic origin, have been used for regeneration of the IVD in vitro and in vivo. Aspects such as ease of use, mechanical properties, regenerative capacity, and their applicability as carriers for regenerative and anti-degenerative factors determine their suitability for IVD regeneration. The current review provides an overview of the biomaterials used with respect to these properties, including their drawbacks. In addition, as biomaterial application until now appears to have been based on a mix of mere availability and intuition, a more rational design is proposed for future use of biomaterials for IVD regeneration. Ideally, high-throughput screening is used to identify optimally effective materials, or alternatively medium content comparative studies should be carried out to determine an appropriate reference material for future studies on novel materials.

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Injectable silk fibroin/polyurethane composite hydrogel for nucleus pulposus replacement

Cited by 60 publications

References 39 publications

A review of the application of reinforced hydrogels and silk as biomaterials for intervertebral disc repair

A review of the application of reinforced hydrogels and silk as biomaterials for intervertebral disc repair

Degradation, water uptake, injectability and mechanical strength of injectable bone substitutes composed of silk fibroin and hydroxyapatite nanorods

Biomaterials for intervertebral disc regeneration: past performance and possible future strategies

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