Injection of ligand-free gold and silver nanoparticles into murine embryos does not impact pre-implantation development

Taylor, Ulrike; Garrels, Wiebke; Barchanski, Annette; Peterson, Svea; Sajti, Laszlo; Lucas‐Hahn, Andrea; Gamrad, Lisa; Baulain, Ulrich; Klein, Sabine; Kues, Wilfried A.; Barcikowski, Stephan; Rath, Detlef

doi:10.3762/bjnano.5.80

Cited by 29 publications

(20 citation statements)

References 75 publications

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“…Unlike silver nanoparticles, the influence of comparable concentrations of Ag+ ions resulted in the immediate arrest of development of the embryo. 20 We obtained data that rates of embryonic mortality had not changed the conditions a ten-time treatment AgNPs (2 mg/kg and 4 mg/kg); our data are consistent with the previous data, confirming the statement that embryos may have potential for the deployment of compensatory mechanisms of action under conditions of silver administration at doses lower than those causing obvious toxicity. 18,20 It is also one more proof to support the hypothesis that the AgNPs toxicity is primarily linked to Ag+ bioavailability.…”

Section: Discussionsupporting

confidence: 81%

“…20 We obtained data that rates of embryonic mortality had not changed the conditions a ten-time treatment AgNPs (2 mg/kg and 4 mg/kg); our data are consistent with the previous data, confirming the statement that embryos may have potential for the deployment of compensatory mechanisms of action under conditions of silver administration at doses lower than those causing obvious toxicity. 18,20 It is also one more proof to support the hypothesis that the AgNPs toxicity is primarily linked to Ag+ bioavailability. It is important to stress that our data suggest that oocytes in mammals have the potential for the deployment of compensatory mechanisms of action under conditions of Ag+ administration at doses lower than those causing obvious toxicity.…”

Section: Discussionsupporting

confidence: 81%

“…19 There are also data that silver nanoparticles do not have any effect on mice embryos. 20 The differences in body weight, food consumption, weight body index pregnancy, fetal death, fetal weight and placental sex ratio, morphological changes between groups have been evaluated. It has been established that the repeated oral administration of AgNPs during pregnancy causes oxidative stress in the liver tissue at ≥100 mg/kg/day, but does not cause changes in the development of embryo and fetus under conditions of administration of doses up to 1000 mg/kg/day.…”

Section: Discussionmentioning

confidence: 99%

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Functional status of reproductive system under treatment of silver nanoparticles in female mice

Lytvynenko

Rieznichenko

Sribna

et al. 2017

Int J Reprod Contracept Obstet Gynecol

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INTRODUCTIONThe unique desired physico-chemical properties of NPs, such as higher stability in the aquatic environment, decreased size and increased specific surface area and thus enhanced reactivity, which makes nanomaterials more efficient in industrial applications and at the same time make them more harmful for living organisms. 1,2 ABSTRACTBackground: The use of silver nanoparticles (AgNP) has increased very significantly in recent years the work in science is currently on focused on assessing human health and environmental risks of nanotechnology. The aim of the present study was to estimate the functional status of the female reproductive system in mice under condition of the intravenous treatment of silver nanoparticles (AgNPs), namely to assess meiotic maturation of oocytes, viability of follicular cells surrounding the oocyte, spontaneous contractile activity of the myometrium and pre-and postimplantation mortality of embryos. Methods: Research (two series) has been done on white laboratory mice (8 weeks, 16-18 g). AgNPs are spherical nanoparticles of 30 nm (8 mg/ml for metal) diluted in water for injection. Method of treatment: intravenous. Two doses (2 mg/kg and 4 mg/kg) have been investigated. Frequency of treatment: one time per day with each dose of 1, 5 and 10 times (n=10 animals in each group). Material for the study (ovaries, uterus) were taken the day after the last AgNPs injection. Results: Ten-time AgNPs treatment (2 mg/kg and 4 mg/kg) results in inhibition of oocytes meiotic maturation in mice; a single-and five-time AgNPs treatment (2 mg/kg and 4 mg/kg) increases the number of apoptotic cells, while the ten-time AgNPs treatment results in an increase of the apoptotic and necrotic follicular cells surrounding oocytes; for the five-and ten-time AgNPs treatment (4 mg/kg) the index of contractility (IC) of the uterus increased; for the ten-time AgNPs treatment (2 mg/kg and 4 mg/kg) no differences in value of the embryonic mortality between control and study groups have been observed. Conclusions: This study suggests that the development of nanomaterials should be safer and non-toxic and the potential reprotoxicity of AgNPs should be investigated more carefully.

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Section: Discussionsupporting

confidence: 81%

Section: Discussionsupporting

confidence: 81%

Section: Discussionmentioning

confidence: 99%

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Functional status of reproductive system under treatment of silver nanoparticles in female mice

Lytvynenko

Rieznichenko

Sribna

et al. 2017

Int J Reprod Contracept Obstet Gynecol

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“…This includes effects on spermatozoa [147] as well as on embryo development [155]. However, utilization of laser-fabricated nanomaterials is not limited to this field but these materials were also applied in a multitude of other toxicological trials.…”

Section: Reviewmentioning

confidence: 99%

Current state of laser synthesis of metal and alloy nanoparticles as ligand-free reference materials for nano-toxicological assays

Rehbock¹,

Jakobi²,

Gamrad³

et al. 2014

Beilstein J. Nanotechnol.

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136

144

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SummaryDue to the abundance of nanomaterials in medical devices and everyday products, toxicological effects related to nanoparticles released from these materials, e.g., by mechanical wear, are a growing matter of concern. Unfortunately, appropriate nanoparticles required for systematic toxicological evaluation of these materials are still lacking. Here, the ubiquitous presence of surface ligands, remaining from chemical synthesis are a major drawback as these organic residues may cause cross-contaminations in toxicological studies. Nanoparticles synthesized by pulsed laser ablation in liquid are a promising alternative as this synthesis route provides totally ligand-free nanoparticles. The first part of this article reviews recent methods that allow the size control of laser-fabricated nanoparticles, focusing on laser post irradiation, delayed bioconjugation and in situ size quenching by low salinity electrolytes. Subsequent or parallel applications of these methods enable precise tuning of the particle diameters in a regime from 4–400 nm without utilization of any artificial surface ligands. The second paragraph of this article highlights the recent progress concerning the synthesis of composition controlled alloy nanoparticles by laser ablation in liquids. Here, binary and ternary alloy nanoparticles with totally homogeneous elemental distribution could be fabricated and the composition of these particles closely resembled bulk implant material. Finally, the model AuAg was used to systematically evaluate composition related toxicological effects of alloy nanoparticles. Here Ag+ ion release is identified as the most probable mechanism of toxicity when recent toxicological studies with gametes, mammalian cells and bacteria are considered.

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“…54–56 It has been suggested that preimplantation embryos are generally more sensitive to toxicants than somatic cells. 57–58 We therefore assessed development of embryos in the presence of nanogels. Zygotes were cultured in KSOM with or without FITC-labeled nanogels (KSOM+NG, Figure 5).…”

Section: Resultsmentioning

confidence: 99%

Polyamide Nanogels from Generally Recognized as Safe Components and Their Toxicity in Mouse Preimplantation Embryos

et al. 2015

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Safe delivery systems that can not only encapsulate hydrophobic drug molecules, but also release them in response to specific triggers, are important in several therapeutic and biomedical applications. In this paper, we have designed a nanogel based on molecules that are generally recognized as safe (GRAS). We have shown that the resultant polymeric nanogels exhibit responsive molecular release, and also show high in vitro cellular viability on HEK 293T, HeLa, MCF 7 and A549 cell lines. The toxicity of these nanogels was further evaluated with a highly sensitive assay using mouse preimplantation embryo development, where blastocysts were formed after four days of in vitro culture and live pups were born when morulae/early blastocysts were transferred to the uteri of surrogate recipients. Our results indicate that these nanogels are non-toxic during mammalian development and do not alter normal growth or early embryo success rate.

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Injection of ligand-free gold and silver nanoparticles into murine embryos does not impact pre-implantation development

Cited by 29 publications

References 75 publications

Functional status of reproductive system under treatment of silver nanoparticles in female mice

Functional status of reproductive system under treatment of silver nanoparticles in female mice

Current state of laser synthesis of metal and alloy nanoparticles as ligand-free reference materials for nano-toxicological assays

Polyamide Nanogels from Generally Recognized as Safe Components and Their Toxicity in Mouse Preimplantation Embryos

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