Ink Jet Three-Dimensional Digital Fabrication for Biological Tissue Manufacturing: Analysis of Alginate Microgel Beads Produced by Ink Jet Droplets for Three Dimensional Tissue Fabrication

Nakamura, Makoto; Nishiyama, Yuichi; Henmi, Chizuka; Iwanaga, Shintaroh; Nakagawa, Hidemoto; Yamaguchi, Kumiko; Akita, Keiichi; Mochizuki, Shuichi; Takiura, Koki

doi:10.2352/j.imagingsci.technol.(2008)52:6(060201)

Cited by 78 publications

(47 citation statements)

References 15 publications

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“…14, 127 To extend its application to 3D bioprinting, Nakamura and others developed an alginate-based bioink with immediate chelation when inkjet printed into a liquid CaCl 2 solution. 78 The chelated alginate creates microgel droplets as building blocks (~25-40 μm diameter). The resulting higher resolution allowed for fabrication of a cell-compatible alginate vessel with a 200 μm diameter and demonstrated feasible printing of computer-designed images.…”

Section: Inkjet Bioprintersmentioning

confidence: 99%

“…The resulting higher resolution allowed for fabrication of a cell-compatible alginate vessel with a 200 μm diameter and demonstrated feasible printing of computer-designed images. 1, 78, 82, 125 On the other hand, Pataky and others combined alginate bioink with a sacrificial calcium-containing substrate (e.g., gelatin) to 3D bioprint an acellular ~200 μm diameter bifurcated vessel. 89 In addition to alginate-based bioink, a thrombin/calcium based bioink has been printed onto a fibrinogen surface to replicate the natural polymerization process in wound healing.…”

Section: Inkjet Bioprintersmentioning

confidence: 99%

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3D Bioprinting for Vascularized Tissue Fabrication

et al. 2016

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3D bioprinting holds remarkable promise for rapid fabrication of 3D tissue engineering constructs. Given its scalability, reproducibility, and precise multi-dimensional control that traditional fabrication methods do not provide, 3D bioprinting provides a powerful means to address one of the major challenges in tissue engineering: vascularization. Moderate success of current tissue engineering strategies have been attributed to the current inability to fabricate thick tissue engineering constructs that contain endogenous, engineered vasculature or nutrient channels that can integrate with the host tissue. Successful fabrication of a vascularized tissue construct requires synergy between high throughput, high-resolution bioprinting of larger perfusable channels and instructive bioink that promotes angiogenic sprouting and neovascularization. This review aims to cover the recent progress in the field of 3D bioprinting of vascularized tissues. It will cover the methods of bioprinting vascularized constructs, bioink for vascularization, and perspectives on recent innovations in 3D printing and biomaterials for the next generation of 3D bioprinting for vascularized tissue fabrication.

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Section: Inkjet Bioprintersmentioning

confidence: 99%

Section: Inkjet Bioprintersmentioning

confidence: 99%

3D Bioprinting for Vascularized Tissue Fabrication

et al. 2016

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“…The volume of ejected droplets is mediated by several operating parameters such as the bioink material characteristics, the printhead geometry (orifice diameter) and its actuation voltage pulse characteristics [23,32,36,43,46,71,82,83]. For example, the width of each M A N U S C R I P T A C C E P T E D ACCEPTED MANUSCRIPT 13 coalesced line in Fig.…”

Section: Droplet-substrate Interactionsmentioning

confidence: 99%

A comprehensive review on droplet-based bioprinting: Past, present and future

2016

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Droplet-based bioprinting (DBB) offers greater advantages due to its simplicity and agility with precise control on deposition of biologics including cells, growth factors, genes, drugs and biomaterials, and has been a prominent technology in the bioprinting community. Due to its immense versatility, DBB technology has been adopted by various application areas, including but not limited to, tissue engineering and regenerative medicine, transplantation and clinics, pharmaceutics and high-throughput screening, and cancer research. Despite the great benefits, the technology currently faces several challenges such as a narrow range of available bioink materials, bioprinting-induced cell damage at substantial levels, limited mechanical and structural integrity of bioprinted constructs, and restrictions on the size of constructs due to lack of vascularization and porosity. This paper presents a first-time review of DBB and comprehensively covers the existing DBB modalities including inkjet, electrohydrodynamic, acoustic, and micro-valve bioprinting. The recent notable studies are highlighted, the relevant bioink biomaterials and bioprinters are expounded, the application areas are presented, and the future prospects are provided to the reader.

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“…Synthetic hydrogel such as polyethylene glycol (PEG), PEG-DA and acrylamide-glycerol are generally cross-linked through UV-ray exposure (Barry et al , 2009, Nakamura, Nishiyama, 2008, Ovsianikov et al , 2011. The stability of the printed structures from these synthetic hydrogels can be controlled by duration or intensity of UV-ray.…”

Section: Accepted Manuscriptmentioning

confidence: 99%