Wearable flexible sensors based on integrated microfluidic networks with multiplex analysis capability are emerging as a new paradigm to assess human health status and show great potential in application fields such as clinical medicine and athletic monitoring. Well‐designed microfluidic sensors can be attached to the skin surface to acquire various pieces of physiological information with high precision, such as sweat loss, information regarding metabolites, and electrolyte balance. Herein, the recent progress of wearable microfluidic sensors for applications in healthcare monitoring is summarized, including analysis principles and microfabrication methods. Finally, the challenges and opportunities for wearable microfluidic sensors in practical applications are discussed.