Inkjet printing of insulin microneedles for transdermal delivery

Ross, Susan; Scoutaris, Nikolaos; Lamprou, Dimitrios A.; Mallinson, David J.; Douroumis, Dennis

doi:10.1007/s13346-015-0251-1

Cited by 82 publications

(36 citation statements)

References 33 publications

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“…Inkjet printing was then employed to apply coatings of insulin and sugar alcohol solutions through a piezoelectric dispenser. This 2D printing approach has been previously used for coating of metal MNs (Ross et al, 2015;Uddin et al, 2015) where by optimizing the applied voltage (mV) and the pulse duration (ms) droplets of 300 pL volume with consistent particle size of 100 -110 m as observed by the printer's stroboscope, were produced. The dispenser's tip (50m) was kept close to the microneedle surface to avoid losses of the coating material.…”

Section: Resultsmentioning

confidence: 99%

“…In this study, to determine the effect of needle shape on the force required for skin penetration, piercing tests using porcine skin were conducted. In order to have a frame of reference with respective literature, metallic MN arrays were tested as well, the fabrication and physical characteristics of which, are described elsewhere (Ross et al, 2015). A texture analyser was employed, and the MN array was mounted on the moving probe using double-sided adhesive tape.…”

Section: Penetration Of Mn In Porcine Skinmentioning

confidence: 99%

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3D printed microneedles for insulin skin delivery

Pere

Economidou

Lall

et al. 2018

International Journal of Pharmaceutics

Self Cite

279

164

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In this study, polymeric microneedle patches were fabricated by stereolithography, a 3D printing technique, for the transdermal delivery of insulin. A biocompatible resin was photopolymerized to build pyramid and cone microneedle designs followed by inkjet print coating of insulin formulations. Trehalose, mannitol and xylitol were used as drug carriers with the aim to preserve insulin integrity and stability but also to facilitate rapid release rates. Circular dichroism and Raman analysis demonstrated that all carriers maintained the native form of insulin, with xylitol presenting the best performance. Franz cell release studies were used for in vitro determination of insulin release rates in porcine skin. Insulin was released rapidly within 30 min irrespectively of the microneedle design. 3D printing was proved an effective technology for the fabrication of biocompatible and scalable microneedle patches.

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Section: Resultsmentioning

confidence: 99%

Section: Penetration Of Mn In Porcine Skinmentioning

confidence: 99%

3D printed microneedles for insulin skin delivery

Pere

Economidou

Lall

et al. 2018

International Journal of Pharmaceutics

Self Cite

279

164

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show abstract

“…Most commonly, aqueous coating solutions are used to create a uniform thin film of material on a metallic MN surface [4,7,[13][14][15]. Whilst these studies have demonstrated the ability to coat hydrophilic drugs and particulates onto MNs, published reports of successful MN coating using hydrophobic materials are limited and typically describe low drug loading doses [15,16].…”

Section: Introductionmentioning

confidence: 99%

Formulation of hydrophobic peptides for skin delivery via coated microneedles

Zhao

Coulman

Hanna

et al. 2017

Journal of Controlled Release

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Microneedles (MNs) have been investigated as a minimally-invasive delivery technology for a range of active pharmaceutical ingredients (APIs). Various formulations and methods for coating the surface of MNs with therapeutics have been proposed and exemplified, predominantly for hydrophilic drugs and particulates. The development of effective MN delivery formulations for hydrophobic drugs is more challenging with dosing restrictions and the use of organic solvents impacting on both the bioactivity and the kinetics of drug release. In this study we propose a novel formulation that is suitable for MN coating of hydrophobic auto-antigen peptides currently being investigated for antigen specific immunotherapy (ASI) of type 1 diabetes. The formulation, comprising three co-solvents (water, 2-methyl-2-butanol and acetic acid) and polyvinylalcohol 2000 (PVA2000) can dissolve both hydrophilic and hydrophobic peptide auto-antigens at relatively high, and clinically relevant, concentrations (25mg/ml or 12.5mg/ml). The drug:excipient ratio is restricted to 10:1 w/w to maximise dose whilst ensuring that the dry-coated payload does not significantly impact on MN skin penetration performance. The coating formulation and process does not adversely affect the biological activity of the peptide. The delivery efficiency of the coated peptide into skin is influenced by a number of parameters. Electropolishing the metal MN surface increases delivery efficiency from 2.0±1.0% to 59.9±6.7%. An increased mass of peptide formulation per needle, from 0.37μg to 2μg peptide dose, resulted in a thicker coating and a 20% reduction in the efficiency of skin delivery. Other important performance parameters for coated MNs include the role of excipients in assisting dissolution from the MNs, the intrinsic hydrophobicity of the peptide and the species of skin model used in laboratory studies. This study therefore both exemplifies the potential of a novel formulation for coating hydrophobic and hydrophilic peptides onto MN devices and provides new insight into the factors that influence delivery efficiency from coated MNs. Importantly, the results provide guidance for identifying critical attributes of the formulation, coating process and delivery device, that confer reproducible and effective delivery from coated MNs, and thus contribute to the requirements of the regulators appraising these devices.

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“…[86] Currently, such technique has been main utilized to coat pre-fabricated microneedles with drugs for the personalized and combined drug loading. [87,88] Photopolymerization-based technique refers to the fabrication of 3D models by selectively polymerization of photo-sensitive polymers under the laser/light irradiation. Such technique could fabricate polymeric microneedles by consistent layer-by-layer polymerization of UV-sensitive polymers through the photopolymerization curing process.…”

Section: Methods For the Fabrication Of Polymeric Microneedlesmentioning

confidence: 99%

Biomedical Applications of Polymeric Microneedles for Transdermal Therapeutic Delivery and Diagnosis: Current Status and Future Perspectives

Liu

Luo

Xing

2020

Advanced Therapeutics

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Transdermal drug delivery is a crucial extension of drug administration routes and has been widely acknowledged as an alternative way to traditional oral administration and subcutaneous injections due to the advantages such as increased dosage efficacy, decreased systemic side effect, and improved patient compliance. The past few decades have witnessed biomedical applications of microneedles in various cutting‐edge fields. Microneedles are needles with micron‐scale length which can pierce the epidermis of the skin in a minimally invasive manner for transdermal drug delivery. Compared with inorganic and metal microneedles, polymeric microneedles have attracted more attention because of their superior biocompatibility, nontoxicity, and biodegradability. In this review, the state‐of‐art and future biomedical applications of polymeric microneedles are summarized. First of all, a brief introduction to the polymeric microneedles, including types of polymeric microneedles and methods for the fabrication is included. Then the biomedical applications of polymeric microneedles in transdermal drug delivery and diagnosis are summarized in detail. Finally, discussions on the current limitations and future perspectives of polymeric microneedles are provided.

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Inkjet printing of insulin microneedles for transdermal delivery

Cited by 82 publications

References 33 publications

3D printed microneedles for insulin skin delivery

3D printed microneedles for insulin skin delivery

Formulation of hydrophobic peptides for skin delivery via coated microneedles

Biomedical Applications of Polymeric Microneedles for Transdermal Therapeutic Delivery and Diagnosis: Current Status and Future Perspectives

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