a b s t r a c tStreams and rivers emit significant amounts of CO 2 and constitute a preferential pathway of carbon transport from terrestrial ecosystems to the atmosphere. However, the estimation of CO 2 degassing based on the water-air CO 2 gradient, gas transfer velocity and stream surface area is subject to large uncertainties. Furthermore, the stable isotope signature of dissolved inorganic carbon (d 13 C-DIC) in streams is strongly impacted by gas exchange, which makes it a useful tracer of CO 2 degassing under specific conditions. For this study, we characterized the annual transfers of dissolved inorganic carbon (DIC) along the groundwater-stream-river continuum based on DIC concentrations, stable isotope composition and measurements of stream discharges. We selected a homogeneous, forested and sandy lowland watershed as a study site, where the hydrology occurs almost exclusively through drainage of shallow groundwater (no surface runoff). We observed the first general spatial pattern of decreases in pCO 2 and DIC and an increase in d 13 C-DIC from groundwater to stream orders 1 and 2, which was due to the experimentally verified faster degassing of groundwater 12 C-DIC compared to 13 C-DIC. This downstream enrichment in 13 C-DIC could be modelled by simply considering the isotopic equilibration of groundwater-derived DIC with the atmosphere during CO 2 degassing. A second spatial pattern occurred between stream orders 2 and 4, consisting of an increase in the proportion of carbonate alkalinity to the DIC accompanied by the enrichment of 13 C in the stream DIC, which was due to the occurrence of carbonate rock weathering downstream. We could separate the contribution of these two processes (gas exchange and carbonate weathering) in the stable isotope budget of the river network. Thereafter, we built a hydrological mass balance based on drainages and the relative contribution of groundwater in streams of increasing order. After combining with the dissolved CO 2 concentrations, we quantified CO 2 degassing for each stream order for the whole watershed. Approximately 75% of the total CO 2 degassing from the watershed occurred in first-and second-order streams. Furthermore, from stream order 2-4, our CO 2 degassing fluxes compared well with those based on stream hydraulic geometry, water pCO 2 , gas transfer velocity, and stream surface area. In first-order streams, however, our approach showed CO 2 fluxes that were twice as large, suggesting that a fraction of degassing occurred as hotspots in the vicinity of groundwater resurgence and was missed by conventional stream sampling.