Inmunohistoquímica en dermatopatología: revisión de los anticuerpos utilizados con mayor frecuencia (parte i)

Fuertes, Laura de Miguel; Santonja, Carlos; Kutzner, Heinz; Requena, Luis

doi:10.1016/j.ad.2012.02.015

Cited by 38 publications

(18 citation statements)

References 63 publications

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“…These antigens constitute good markers of secreting sebum cells, and it is known that cytokeratins 5/6 are expressed on squamous epithelium basal cells while Muc1 may be reactive on some of them. 22 , 23 In our study, both markers were found only in post-natally skin at sebaceous gland and in some cases at basal keratinocytes. Our results show that cytokeratins 5/6 and Muc1 were expressed at sebum glands and at some basal keratinocytes belonging to neonate and adult specimens while neither Muc1 nor cytokeratins 5/6 were detected on embryonic epidermis.…”

Section: Discussionsupporting

confidence: 40%

Muc5ac mucin expression during rat skin development

et al. 2015

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Some mucin genes have been detected during human embryonic and fetal organ development; however, little is known about mucin expression in epidermal development, neither in humans nor in other species. The present research was developed to explore Muc5ac skin expression during pre- and post-natal rat development. Immunohistochemistry (IHC), Western blotting (WB) and RT-PCR were employed. By IHC, Muc5ac protein was found early in embryonic epidermis from day 13 of gestation until seven days after birth when the surface epidermis became negative and the reaction was restricted to secreting sebum cells. In coincidence with IHC findings, WB analysis showed a band at approximately 200KDa at the same periods of development. Results were also confirmed by RT-PCR.Muc5ac expression in rat embryonic epidermis suggests that Muc5ac may play a protective role in embryonic skin previous to birth which may be replaced by pile covering. To our knowledge, this is the first report that confirmed Muc5ac expression during skin development.

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Section: Discussionsupporting

confidence: 40%

Muc5ac mucin expression during rat skin development

et al. 2015

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“…In the case we are presenting, rare papillary mesenchymal bodies representing abortive follicular papillae and evocative of the relationship between primitive hair germ and the papilla, were presumably identified by their morphological characteristics and by lack of positivity for p63, consistent with that reported by other authors in dogs [16]. Trichoblastoma is an epithelial neoplasm deriving from basal cells, that expresses markers for epithelial and basal origin such as CK AE1/AE3, CK5/6, CK 34 beta E12, and p63, both in human and in veterinary medicine [17,18]. Trichoblastoma neoplastic cells of the present case stained positively for cytokeratins and p63, showing staining features similar to what reported in the literature (Figure 4A,B).…”

Section: Discussionsupporting

confidence: 87%

Histological and Immunohistochemical Features of Trichoblastoma in a Sarda Breed Sheep

Polinas

Burrai

Vitiello

et al. 2020

Animals

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Skin tumors with adnexal differentiation are commonly reported in dogs and cats, while only anecdotal evidence is available in sheep. Here we illustrate the macroscopic, histologic, and immunohistochemical features of a cutaneous lesion with adnexal differentiation in a 6-year-old female Sarda breed sheep, surgically treated for a horn-like mass located in the left pinna. Additionally, we investigate a possible contribution of Ovine Papillomaviruses (OaPVs). Histologically, the dermis was expanded by an expansive and unencapsulated multilobulated nodule composed of cuboidal to spindle basaloid cells arranged in variably-sized cytokeratins (CK) AE1-AE3, CK 5/6 and CK 34 beta E12, p63—positive winding cords with a characteristic palisade arrangement of neoplastic cells in the periphery of the tumor. Based on these results, the cutaneous neoplasm was diagnosed as a trabecular trichoblastoma with spindle cells and rare structures resembling papillary mesenchymal bodies. Additionally, multiple enlarged sebaceous lobules clustered around dilated ducts suggestive of sebaceous gland hyperplasia were detected near the trichoblastoma. No PV DNA was found in the examined tissues, suggesting that ovine PVs are not involved in the pathogenesis of the present skin tumors with adnexal differentiation. Further investigations and efforts are required to elucidate the prevalence of skin tumors with adnexal differentiation in this species.

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“…In our case, the stromal cells showed myoepithelial differentiation, illustrated by being positive to S100 and vimentin. [1113]…”

Section: Discussionmentioning

confidence: 99%

Pleomorphic adenoma of the eyelid with apocrine gland origin; an atypical location

Rothwell

Campelos

Prazeres

2016

J Ophthalmic Vis Res

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Purpose:To describe the clinical presentation and treatment of a patient with a cutaneous pleomorphic adenoma of the eyelid.Case Report:A 73-year-old male patient presented with a nodular mass on the lateral third of his right upper eyelid, which had slowly enlarged over 10 years. Radiologic features were of an extra-conical mass, with no invasion of adjacent structures. An excisional biopsy of the lesion was performed. The histopathological examination revealed a biphasic tumor, composed of tubules with a double layer of epithelial cells arranged in a chondromyxoid stroma. The inner epithelial cells were positive for pancytokeratins AE1/AE3 and carcinoembryonic antigen. The outer epithelial cells and stromal component expressed vimentin and S100 protein. These pathologic findings were consistent with a palpebral pleomorphic adenoma, with an apocrine gland origin.Conclusion:Pleomorphic adenomas of the skin are rare tumors, and even less frequent as tumors of the ocular adnexa. These lesions should be considered in the differential diagnosis of palpebral nodular masses, and complete excision should be attempted due to the possibility of malignant transformation.

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Inmunohistoquímica en dermatopatología: revisión de los anticuerpos utilizados con mayor frecuencia (parte i)

Cited by 38 publications

References 63 publications

Muc5ac mucin expression during rat skin development

Muc5ac mucin expression during rat skin development

Histological and Immunohistochemical Features of Trichoblastoma in a Sarda Breed Sheep

Pleomorphic adenoma of the eyelid with apocrine gland origin; an atypical location

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