Innate immune activation of swine intestinal epithelial cell lines (IPEC-J2 and IPI-2I) in response to LPS from Salmonella typhimurium

Arce, Cristina; Ramírez-Boo, M.; Lucena, Carlos; Garrido, Juan J.

doi:10.1016/j.cimid.2008.08.003

Cited by 91 publications

(69 citation statements)

References 62 publications

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“…Furthermore, mRNA expression of has been confi rmed in IPEC-J2 cells. TLR-2, -3, -5, -9 and IL-6, -8 proteins were also detected in these cells (Brosnahan and Brown 2012 ;Arce et al 2010 ;Burkey et al 2009 ).…”

Section: Special Features/morphology/receptorsmentioning

confidence: 91%

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The IPEC-J2 Cell Line

Vergauwen

2015

The Impact of Food Bioactives on Health

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IPEC-J2 cells are intestinal porcine enterocytes isolated from the jejunum of a neonatal unsuckled piglet. The IPEC-J2 cell line is unique as it is derived from the small intestine and is neither transformed nor tumorigenic in nature. IPEC-J2 cells mimic the human physiology more closely than any other cell line of nonhuman origin. Therefore, it is an ideal tool to study epithelial transport, interactions with enteric bacteria, effects of probiotic microorganisms and the effect of nutrients and other feedstuffs on a variety of widely used parameters (e.g. transepithelial electrical resistance (TEER), permeability, metabolic activity) refl ecting epithelial functionality.IPEC-J2 cells undergo in culture a process of spontaneous differentiation that leads to the formation of a polarized monolayer with low or high TEER, depending on the type of serum added to the culture medium, within 1-2 weeks. Porcine serum gives rise to low resistance and normal active transport rates, enabling comparison with the in vivo situation. The high resistance caused by fetal bovine serum can be benefi cial to use when investigating compounds having a negative effect on the monolayer permeability or tight junction structures.There are still many opportunities for exploring the use of these cells as the available research is limited. This chapter will cover the origin, characteristics and methods of the use of IPEC-J2 cells as an in vitro model for several research applications, as well as comparisons between IPEC-J2 cells and other epithelial cell lines.

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Section: Special Features/morphology/receptorsmentioning

confidence: 91%

“…IPEC-J2, IPEC-1 and IPI-2I are three widely used porcine small intestinal cell lines (Arce et al 2010 ). IPI-2I cells have been transformed with an SV40 plasmid, whereas IPEC-J2 and IPEC-1 are non-transformed cell lines.…”

Section: Relevance To Human In Vivo Situationmentioning

confidence: 99%

The IPEC-J2 Cell Line

Vergauwen

2015

The Impact of Food Bioactives on Health

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“…Besides constituting a physical barrier, IECs sense pathogens through pattern recognition receptors (PRRs), such as Toll-like receptors (TLRs), and secrete several signaling cytokines and chemokines (11). Arce et al (2) showed that recognition of Salmonella lipopolysaccharide (LPS) by TLR4 of intestinal IPI-2I cells increases proinflammatory mRNA for TNF-␣ and CXCL8 (neutrophil recruitment) and slightly upregulates CCL2 (monocyte chemotaxis). The present data update our knowledge about IEC response after Salmonella exposure.…”

Section: Discussionmentioning

confidence: 99%

β-Galactomannan and Saccharomyces cerevisiae var. boulardii Modulate the Immune Response against Salmonella enterica Serovar Typhimurium in Porcine Intestinal Epithelial and Dendritic Cells

Badía¹,

Brufau²,

Guerrero-Zamora³

et al. 2012

Clin. Vaccine Immunol.

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Salmonella enterica serovar Typhimurium is a facultative intracellular pathogen that causes inflammation, necrosis, and diarrhea in pigs, as well as being an important source of food-borne diseases in humans. Probiotics and prebiotics are promising alternatives to antibiotics to control and prevent intestinal infections. The present work investigated a recently developed ␤-galactomannan (␤GM) prebiotic compared to the proven probiotic Saccharomyces cerevisiae var. boulardii on porcine ileum intestinal epithelial cells (IECs) of the IPI-2I line and monocyte-derived dendritic cells (DCs) cocultured in vitro with Salmonella. We observed that both S. cerevisiae var. boulardii and ␤GM inhibited the association of Salmonella with IECs in vitro. Our data indicated that ␤GM has a higher ability than S. cerevisiae var. boulardii to inhibit Salmonella-induced proinflammatory mRNA (cytokines tumor necrosis factor alpha [TNF-␣], interleukin-1␣ [IL-1␣], IL-6, and granulocyte-macrophage colony-stimulating factor [GM-CSF] and chemokines CCL2, CCL20, and CXCL8) and at protein levels (IL-6 and CXCL8). Additionally, ␤GM and S. cerevisiae var. boulardii induced some effects on DCs that were not observed on IECs: ␤GM and S. cerevisiae var. boulardii showed slight upregulation of mRNA for TNF-␣, GM-CSF, and CCR7 receptor on porcine monocyte-derived dendritic cells (DCs). Indeed, the addition of ␤GM or S. cerevisiae var. boulardii on DCs cocultured with Salmonella showed higher gene expression (mRNA) for TNF-␣, GM-CSF, and CXCL8 compared to that of the control with Salmonella. In conclusion, the addition of ␤GM inhibits Salmonella-induced proinflammatory profiles in IECs but may promote DC activation, although associated molecular mechanisms remain to be elucidated.

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“…By comparing the cytokine profile, clarify whether HDRsEf1 could be an antagonist to regulate intestine inflammation response to intestine pathogen. In addition to providing physical barrier, intestinal epithelial cells are able to express mediators of inflammation, such as cytokines, chemokines, and other signal molecules, such as TLRs (Arce et al 2010;Kajikawa et al 2011). TLRs are a component of the innate immune system, which can recognize the invading microbes and initiate non-specific immune response (Roger et al 2010).…”

Section: Discussionmentioning

confidence: 99%

The inflammation regulation effects ofEnterococcus faeciumHDRsEf1 on human enterocyte-like HT-29 cells

Tian

Yang

et al. 2016

Animal Cells and Systems

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Enterococcus faecium HDRsEf1 strain used as a probiotic to inhibit intestine inflammation and improve animal growth performance has been proved by our research team; however, it remains unclear how HDRsEf1 was recognized by intestine cells and how it activates the downstream pathway which benefit intestine health. In this study, HDRsEf1 was used to stimulate HT-29 cell line to partially uncover the intestine benefit mechanism of HDRsEf1. The results of cell viability assays showed that HDRsEf1 had no toxicity on HT-29 at concentrations up to 1 × 10 8 CFU/mL, HDRsEf1 could upregulate the TLR1, TLR2, and TLR6 mRNA level, especially TLR2, and significantly downregulate the mRNA level of TLR4, TLR5, TLR7, TLR8, but did not significantly affect the mRNA or protein level of MyD88, which suggests that HDRsEf1 activates the TLR2 pathway in an MyD88-independent pattern. HDRsEf1 could significantly downregulate the mRNA level of pro-inflammatory factors IL-1β, IL-6, IL-8, IL-12p35, IL-17, and TNF-α and did not affect the anti-inflammatory factors IL-10, PPAR-γ, and TSLP; besides HDRsEf1 did not upregulate the degradation of IκB in HT-29 cells. By contrast, enterohemorrhagic E. coli (EHEC) O157:H7 strongly up-regulated the mRNA level of pro-inflammatory factors IL-1β, IL-6, IL-8, IL-23, and TNF-α, downregulated obviously anti-inflammatory factor PPAR-ɣ, and obviously upregulated the degradation of IκB, which suggested that HDRsEf1 may act as an antagonist to regulate intestine inflammation response to intestine pathogen. These findings shed a light on the intestine benefit mechanism of HDRsEf1. ARTICLE HISTORY

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Innate immune activation of swine intestinal epithelial cell lines (IPEC-J2 and IPI-2I) in response to LPS from Salmonella typhimurium

Cited by 91 publications

References 62 publications

The IPEC-J2 Cell Line

The IPEC-J2 Cell Line

β-Galactomannan and Saccharomyces cerevisiae var. boulardii Modulate the Immune Response against Salmonella enterica Serovar Typhimurium in Porcine Intestinal Epithelial and Dendritic Cells

The inflammation regulation effects ofEnterococcus faeciumHDRsEf1 on human enterocyte-like HT-29 cells

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