Inner Anti-inflammatory Mechanisms of Petroleum Ether Extract from Melilotus suaveolens Ledeb

Zhao, Lei; Tao, Junyan; Zhang, Shuling; Pang, Ran; Jin, Feng; Dong, Jing; Guo, Yangyang

doi:10.1007/s10753-007-9039-x

Cited by 43 publications

(22 citation statements)

References 16 publications

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“…Kamei et al (36) reported that vincristine administration decreased the levels of NO metabolites, cGMP and protein levels of neuronal NOS in the spinal cord, and a NOS inhibitor reversed the analgesic effect of a NOS substrate, while Bujalska and Gumułka reported that NOS inhibitors prevented the development of hyperalgesia induced by vincristine administration (37). In an inflammatory cellular model, Melilotus extract decreased the secretion of NO and expression of inducible NOS in a dose-dependent manner (38). The role that the effect of Melilotus extract on NO and NOS plays in the treatment of vincristine-induced neuropathy is unclear.…”

Section: Discussionmentioning

confidence: 99%

Anti-allodynic effect of theoesberiven F in a vincristine-induced neuropathy model

Bang

Kim

Jeong

2016

Experimental and Therapeutic Medicine

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Abstract. Allodynia is a main symptom of the peripheral neuropathy induced by vincristine treatment. Theoesberiven F, a combination of Melilotus extract and proxyphylline, is currently used for the treatment of inflammatory conditions due to its potent anti-inflammatory and analgesic properties. The anti-allodynic effect of theoesberiven F on mechanical and cold allodynia in a rat model of vincristine-induced neuropathy was investigated in the present study. Intraperitoneal vincristine injections were administered to male Sprague-Dawley rats at a dose of 0.1 mg/kg/day over 12 days (5 days of injection, a 2-day cessation and 5 days of injection). Rats that were allodynic following the vincristine injections were randomly allocated into four groups. Normal saline was injected into rats in the control group. Theoesberiven F (0.1, 0.25 and 0.5 mg/kg) was administered to rats in the three experimental groups, respectively. Mechanical and cold allodynia were measured at preadministration and at 15,30, 60, 90, 120, 150 and 180 min following the intraperitoneal administration of normal saline or theoesberiven F. The decreased paw withdrawal threshold induced by vincristine injection was increased by theoesberiven F administration. The increased withdrawal frequency to cold stimuli developed by vincristine was reduced by theoesberiven F administration. The results of this study suggest that the administration of theoesberiven F may be beneficial in reducing the mechanical and cold allodynia developed during vincristine treatment.

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Section: Discussionmentioning

confidence: 99%

Anti-allodynic effect of theoesberiven F in a vincristine-induced neuropathy model

Bang

Kim

Jeong

2016

Experimental and Therapeutic Medicine

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“…The development of molecular research into inflammation [40,41] prompted us to take LPS-stimulated RAW 264.7 cells as a cell model to explore the molecular anti-inflammatory effects of ethanol extract from K. striata (Thunb.) Schindl ethanol extract on IL-1β, IL-6, IL-10, TNF-α, COX-2 and NFkB.…”

Section: Discussionmentioning

confidence: 99%

Anti-inflammatory Effects of Ethanol Extract from Kummerowia striata (Thunb.) Schindl on LPS-Stimulated RAW 264.7 Cell

Tao

Zhao

Huang³

et al. 2008

Inflammation

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Kummerowia striata (Thunb.) Schindl has long been used as a fork herb in inflammation-related therapy. This study was undertaken to determine the anti-inflammatory effect of the plant. High performance liquid chromatography (HPLC) was used for evaluating the extract. While dexamethasone (DM) was used as a positive control, the effects of ethanol extract on the production of IL-1beta, IL-6, NO, COX-2 and TNF-alpha, the expression of iNOS mRNA, TNF-alpha mRNA, COX-2 mRNA, protein production of COX-2 and HO-1, NF-kappaB and I-kappaB of LPS-stimulated RAW 264.7 cells were studied by sandwich ELISA, real-time PCR, Western blot analysis and immunocytochemistry assay respectively. The results showed that K. striata (Thunb.) Schindl had a good anti-inflammatory effect on LPS-stimulated RAW264.7 cell. On one hand, it could significantly inhibit the production of IL-1beta, IL-6, NO, TNF-alpha, COX-2 in LPS-stimulated cell than that of single LPS stimulated cell (p < 0.01 or p < 0.05). On the other hand, it could increase the production of IL-10 and HO-1 than that of single LPS intervention cell (p < 0.01 or p < 0.05). Furthermore, the extract also could inhibit the production of NF-kappaB and I-kappaB compared to single LPS stimulated cell. In a word, it suggested that the anti-inflammatory actions of K. striata (Thunb.) Schindl ethanol extract might be due to the down-regulation of IL-1beta, IL-6, NO, TNF-alpha and COX-2 via the suppression of NF-kappaB activation and conversation of I-kappaB production, and another pathway was up regulating the production of IL-10 and HO-1.

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“…The procedure has been reported in our previous studies (8). Cells were inoculated into 96-well plate with the density of 1x 10 4 • Each group had 8 wells, and the volume ofeach well was 200 ul, The cells were incubated for 24 h under the condition of 37°C, 5% COz' Then the extracts of cynanchum paniculatum with different concentrations were added.…”

Section: Mrt Assay On Cytotoxicitymentioning

confidence: 99%

Protective Activity of the Ethanol Extract of Cynanchum Paniculatum (Bunge) Kitagawa on Treating Herpes Simplex Encephalitis

Guo

Zhang

et al. 2012

Int J Immunopathol Pharmacol

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The first two authors contributed equally to this study To date there has been no valid treatment for herpes simplex encephalitis (HSV). This study explores the protective activity of ethanol extract of Cynanchum paniculatum (bunge) kitagawa for treatment of HSV. Cell models and animal models were established and divided into 4 groups: normal group, virus group, cynanchum paniculatum group and Dexamethasone group. Flow cytometry was employed to detect apoptosis of cell model and TUNEL assay was chosen to detect apoptosis of animal tissues. The survival time of the animal models was observed. ELISA was used to measure TNF-a expression and the Greiss method to measure Nitric Oxide (NO) expression in the mouse brain. As a result, it was found that extract of Cynanchum paniculatum can improve the survival rate of HSV-infected mice. The extract could prevent apoptosis in the neuron cell model and reduce apoptosis rate in brain tissue after HSV infection. With the extract intervention, TNF-a and NO levels in brain tissue were significantly decreased in the animal model. In conclusion, the extract of Cynanchum paniculatum can prevent HSVinducing impairment in the cell and animal model of HSE.Herpes simplex encephalitis (HSE) is a kind of viral encephalitis that attacks neonates as well as older children and adults (l) and is a significant cause of morbidity and mortality (2). Nowadays, the

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Inner Anti-inflammatory Mechanisms of Petroleum Ether Extract from Melilotus suaveolens Ledeb

Cited by 43 publications

References 16 publications

Anti-allodynic effect of theoesberiven F in a vincristine-induced neuropathy model

Anti-allodynic effect of theoesberiven F in a vincristine-induced neuropathy model

Anti-inflammatory Effects of Ethanol Extract from Kummerowia striata (Thunb.) Schindl on LPS-Stimulated RAW 264.7 Cell

Protective Activity of the Ethanol Extract of Cynanchum Paniculatum (Bunge) Kitagawa on Treating Herpes Simplex Encephalitis

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