Innervation of blood vessels in the vomeronasal complex of the rat

Soler, María Valeria Cantó; Suburo, Ángela M.

doi:10.1016/s0006-8993(98)00923-8

Cited by 12 publications

(1 citation statement)

References 32 publications

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“…The VNO lysates used in these experiments contained both of these subcellular elements, consistent with previous expression patterns for Homer. The non-sensory portions of the VNO are densely innervated by the autonomic nervous system [3,32] and the sensory microvilli contain a well-explored calcium-signalling pathway (Fig. 8) [1,14,18].…”

Section: Discussionmentioning

confidence: 99%

The TRPC2 channel forms protein-protein interactions with Homer and RTP in the rat vomeronasal organ

2010

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BackgroundThe signal transduction cascade operational in the vomeronasal organ (VNO) of the olfactory system detects odorants important for prey localization, mating, and social recognition. While the protein machinery transducing these external cues has been individually well characterized, little attention has been paid to the role of protein-protein interactions among these molecules. Development of an in vitro expression system for the transient receptor potential 2 channel (TRPC2), which establishes the first electrical signal in the pheromone transduction pathway, led to the discovery of two protein partners that couple with the channel in the native VNO.ResultsHomer family proteins were expressed in both male and female adult VNO, particularly Homer 1b/c and Homer 3. In addition to this family of scaffolding proteins, the chaperones receptor transporting protein 1 (RTP1) and receptor expression enhancing protein 1 (REEP1) were also expressed. RTP1 was localized broadly across the VNO sensory epithelium, goblet cells, and the soft palate. Both Homer and RTP1 formed protein-protein interactions with TRPC2 in native reciprocal pull-down assays and RTP1 increased surface expression of TRPC2 in in vitro assays. The RTP1-dependent TRPC2 surface expression was paralleled with an increase in ATP-stimulated whole-cell current in an in vitro patch-clamp electrophysiological assay.ConclusionsTRPC2 expression and channel activity is regulated by chaperone- and scaffolding-associated proteins, which could modulate the transduction of chemosignals. The developed in vitro expression system, as described here, will be advantageous for detailed investigations into TRPC2 channel activity and cell signalling, for a channel protein that was traditionally difficult to physiologically assess.

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Section: Discussionmentioning

confidence: 99%

The TRPC2 channel forms protein-protein interactions with Homer and RTP in the rat vomeronasal organ

2010

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Diversity of the vomeronasal system in mammals: The singularities of the sheep model

Salazar

Sánchez‐Quinteiro

Alemañ

et al. 2007

Microscopy Res & Technique

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The enormous morphological diversity and heterogeneity of the vomeronasal system (VNS) in mammals--as well as its complete absence in some cases--complicates the extrapolation of data from one species to another, making any physiological and functional conclusions valid for the whole Mammalian Class difficult and risky to draw. Some highly-evolved macrosmatic mammals, like sheep, have been previously used in interesting behavioral studies concerning the main and accessory olfactory systems. However, in this species, certain crucial morphological peculiarities have not until now been considered. Following histological, histochemical and immunohistochemical procedures, we have studied the vomeronasal organ (VNO) and the accessory olfactory bulb (AOB) of adult sheep. We have determined: (1) that all structures which classically define the VNO in mammals are present and well developed, providing the morphological basis for functional activity. (2) that, conversely, there is only a scant population of scattered mitral/tufted cells. One morphological consequence of both details is that the strata of the AOB in adult sheep are not as sharply defined as in other species; moreover, the small number of the mitral/tufted cells in the AOB may imply that the VNS of adult sheep is not capable of functioning in the way a well-developed VNS does in other species. (3) the zone to zone projection from the apical and basal sensory epithelium of the VNO to the anterior and posterior part of the AOB, respectively, typical in rodents, lagomorphs and marsupials, is not present in adult sheep.

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Neuropeptides in sensory signal processing

Carr

Frings

2018

Cell Tissue Res

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Innervation of blood vessels in the vomeronasal complex of the rat

Cited by 12 publications

References 32 publications

The TRPC2 channel forms protein-protein interactions with Homer and RTP in the rat vomeronasal organ

The TRPC2 channel forms protein-protein interactions with Homer and RTP in the rat vomeronasal organ

Diversity of the vomeronasal system in mammals: The singularities of the sheep model

Neuropeptides in sensory signal processing

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